Marigold Supercritical Extract as Potential Co-adjuvant in Pancreatic Cancer: The Energetic Catastrophe Induced via BMP8B Ends Up With Autophagy-Induced Cell Death

Cedrón, Marta Gómez de; Mouhid, Lamia; García-Carrascosa, Elena; Fornari, Tiziana; Reglero, Guillermo; Molina, Ana Ramı́rez de

doi:10.3389/fbioe.2019.00455

Cited by 12 publications

(10 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We monitored mitochondrial respiration and glycolytic function using XF Cell Mito Stress Test kit and XF Glycolysis Stress kit according to manufacturer instructions (Seahorse Biosciences). Briefly, for GlycoStress assay, 20 000 cells were seeded in an XFe96 well plate and maintained in DMEM 10% FBS to allow the cells to attach [36]. Then, the culture medium was changed to 2 m m glutamine without glucose in XFe DMEM (5 m m Hepes) to starve the cells for 1 h. First basal extracellular acidification rate (ECAR) was determined (1–3 measurements).…”

Section: Methodsmentioning

confidence: 99%

“…Next, maximal glycolytic capacity was assayed (7–9 measurements) after the injection of oligomycin, which blocks the ATP production from the oxidative mitochondrial respiration. Finally, a third injection with 50 m m DG final concentration was done to shut down aerobic glycolysis (10–12 measurements) [36]. For MitoStress assay, 40 000 cells were seeded in an XFe96 well plate, and cells were maintained in DMEM 10% FBS to allow the cells to attach.…”

Section: Methodsmentioning

confidence: 99%

“…Aerobic glycolysis (Cell GlycoStress Test) and mitochondrial oxidative respiration (Cell MitoStress Test) were examined with the XFe96 Cell Bionalyzer (Seahorse Biosciences, North Billerica, MA, USA, XFe96). Optimal cell density and drugs titration were determined as previously described [36]. The requirements of the cells for aerobic glycolysis and oxidative phosphorylation were screened after the injection of several modulators of both bioenergetic pathways.…”

Section: Extracellular Flux Analysis Of the Acidification Rate (Ecar)mentioning

confidence: 99%

“…Three different modulators of the mitochondrial respiration were sequentially introduced. After basal oxygen consumption rate (OCR) determination (1-3 measurements), oligomycin (1 µM), which inhibits ATPase, was added to determine the amount of oxygen dedicated to ATP production by mitochondria (3-6 measurements) [36].…”

Section: Extracellular Flux Analysis Of the Acidification Rate (Ecar)mentioning

confidence: 99%

See 3 more Smart Citations

Metabolic enzyme ACSL3 is a prognostic biomarker and correlates with anticancer effectiveness of statins in non‐small cell lung cancer

et al. 2020

Self Cite

View full text Add to dashboard Cite

Lung cancer is one of the most common cancers, still characterized by high mortality rates. As lipid metabolism contributes to cancer metabolic reprogramming, several lipid metabolism genes are considered prognostic biomarkers of cancer. Statins are a class of lipid-lowering compounds used in treatment of cardiovascular disease that are currently studied for their antitumor effects. However, their exact mechanism of action and specific conditions in which they should be administered remains unclear. Here, we found that simvastatin treatment effectively promoted antiproliferative effects and modulated lipid metabolism-related pathways in non-small cell lung cancer (NSCLC) cells and that the antiproliferative effects of statins were potentiated by overexpression of acyl-CoA synthetase long-chain family member 3 (ACSL3). Moreover, ACSL3 overexpression was associated with worse clinical outcome in patients with high-grade NSCLC. Finally, we found that patients with high expression levels of ACSL3 displayed a clinical benefit of statins treatment. Therefore, our study highlights ACSL3 as a prognostic biomarker for NSCLC, useful to select patients who would obtain a clinical benefit from statin administration.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Extracellular Flux Analysis Of the Acidification Rate (Ecar)mentioning

confidence: 99%

Section: Extracellular Flux Analysis Of the Acidification Rate (Ecar)mentioning

confidence: 99%

See 2 more Smart Citations

Metabolic enzyme ACSL3 is a prognostic biomarker and correlates with anticancer effectiveness of statins in non‐small cell lung cancer

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Precision nutrition can be considered as a therapeutic tool against chronic diseases related to metabolism, including cancer. This way, diet-derived ingredients, bioactive compounds from natural sources, and nutritional strategies can be developed as co-adjuvants in combination with the clinical therapies in cancer treatment [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Saponin-Rich Extracts and Their Acid Hydrolysates Differentially Target Colorectal Cancer Metabolism in the Frame of Precision Nutrition

Cedrón

Hierro

Reguero

et al. 2020

Cancers

Self Cite

View full text Add to dashboard Cite

Saponins or their aglycone form, sapogenin, have recently gained interest as bioactive agents due to their biological activities, their antitumoral effects being among them. Metabolic reprogramming has been recognized as a hallmark of cancer and, together with the increased aerobic glycolysis and glutaminolysis, the altered lipid metabolism is considered crucial to support cancer initiation and progression. The purpose of this study was to assess and compare the inhibitory effects on colorectal cancer cell lines of saponin-rich extracts from fenugreek and quinoa (FE and QE, respectively) and their hydrolyzed extracts as sapogenin-rich extracts (HFE and HQE, respectively). By mean of the latest technology in the analysis of cell bioenergetics, we demonstrate that FE and HFE diminished mitochondrial oxidative phosphorylation and aerobic glycolysis; meanwhile, quinoa extracts did not show relevant activities. Distinct molecular mechanisms were identified for fenugreek: FE inhibited the expression of TYMS1 and TK1, synergizing with the chemotherapeutic drug 5-fluorouracil (5-FU); meanwhile, HFE inhibited lipid metabolism targets, leading to diminished intracellular lipid content. The relevance of considering the coexisting compounds of the extracts or their hydrolysis transformation as innovative strategies to augment the therapeutic potential of the extracts, and the specific subgroup of patients where each extract would be more beneficial, are discussed in the frame of precision nutrition.

show abstract

UHPLC Q-Exactive MS-based spleen metabolomics and lipidomics to explore the effect mechanisms of Danggui Buxue Decoction in anemia mice

et al. 2020

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Marigold Supercritical Extract as Potential Co-adjuvant in Pancreatic Cancer: The Energetic Catastrophe Induced via BMP8B Ends Up With Autophagy-Induced Cell Death

Cited by 12 publications

References 47 publications

Metabolic enzyme ACSL3 is a prognostic biomarker and correlates with anticancer effectiveness of statins in non‐small cell lung cancer

Metabolic enzyme ACSL3 is a prognostic biomarker and correlates with anticancer effectiveness of statins in non‐small cell lung cancer

Saponin-Rich Extracts and Their Acid Hydrolysates Differentially Target Colorectal Cancer Metabolism in the Frame of Precision Nutrition

UHPLC Q-Exactive MS-based spleen metabolomics and lipidomics to explore the effect mechanisms of Danggui Buxue Decoction in anemia mice

Contact Info

Product

Resources

About