Marine actinobacteria: new opportunities for natural product search and discovery

Bull, Alan T.; Stach, James E. M.

doi:10.1016/j.tim.2007.10.004

Cited by 338 publications

(244 citation statements)

References 72 publications

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“…Increasingly more marine Actinobacteria are being discovered (Bull and Stach, 2007) and as shown here are also abundant in microbial mats. In contrast to several other phyla, the numbers of sequence reads related to Actinobacteria were more equally distributed over the three stations with the highest content in ST2 (14%) and lowest in ST3 (9%).…”

Section: Functional Diversitymentioning

confidence: 53%

Analysis of bacterial and archaeal diversity in coastal microbial mats using massive parallel 16S rRNA gene tag sequencing

2011

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Coastal microbial mats are small-scale and largely closed ecosystems in which a plethora of different functional groups of microorganisms are responsible for the biogeochemical cycling of the elements. Coastal microbial mats play an important role in coastal protection and morphodynamics through stabilization of the sediments and by initiating the development of salt-marshes. Little is known about the bacterial and especially archaeal diversity and how it contributes to the ecological functioning of coastal microbial mats. Here, we analyzed three different types of coastal microbial mats that are located along a tidal gradient and can be characterized as marine (ST2), brackish (ST3) and freshwater (ST3) systems. The mats were sampled during three different seasons and subjected to massive parallel tag sequencing of the V6 region of the 16S rRNA genes of Bacteria and Archaea. Sequence analysis revealed that the mats are among the most diverse marine ecosystems studied so far and consist of several novel taxonomic levels ranging from classes to species. The diversity between the different mat types was far more pronounced than the changes between the different seasons at one location. The archaeal community for these mats have not been studied before and revealed a strong reaction on a short period of draught during summer resulting in a massive increase in halobacterial sequences, whereas the bacterial community was barely affected. We concluded that the community composition and the microbial diversity were intrinsic of the mat type and depend on the location along the tidal gradient indicating a relation with salinity.

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Section: Functional Diversitymentioning

confidence: 53%

Analysis of bacterial and archaeal diversity in coastal microbial mats using massive parallel 16S rRNA gene tag sequencing

2011

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“…Poorly explored areas like the Antarctic present significant potential for the discovery of novel microorganisms like bacteria and biological active metabolites (Moncheva et al, 2002;Marinelli et al, 2004;Tindall, 2004;Taton et al, 2006;Bull and Stach, 2007). Barrientos Island is located at 62°24'S, 59°47'W, at the north entrance to the English Strait between Greenwich and Robert Islands.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Antarctic proteobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites

et al. 2012

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ABSTRACT. Fifty-seven proteobacterium species were successfully isolated from soils of Barrientos Island of the Antarctic using 11 different isolation media. Analysis of 16S rDNA sequencing of these isolates showed that they belonged to eight different genera, namely Bradyrhizobium, Sphingomonas, Methylobacterium, Caulobacter, Paracoccus, Ralstonia, Rhizobium, and Staphylococcus. All isolates were studied for capability of producing antimicrobial and antifungal secondary metabolites using high-throughput screening models. Approximately 23 (13/57) and 2% (1/57) of isolates inhibited growth of Candida albicans ATCC 10231 T and Staphylococcus aureus ATCC 51650 T , respectively. These results indicated that proteobacterium species isolates from Antarctic could serve as potential source of useful bioactive metabolites. Enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting produced nine clusters and 13 single isolates, with a high D value of 0.9248. RAPD fingerprinting produced six clusters and 13 single isolates, with a relatively low D value of 0.7776. ERIC-PCR analysis proved to have better discrimination capability than RAPD analysis and generated better clustering for all proteobacterium species isolates. We conclude that ERIC-PCR is a robust, reliable and rapid molecular typing method for discriminating different genera of proteobacteria.

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“…The marine environment is proving to be a major source of new natural products including first-in-a-class drug candidates [2] and is the focus of much of our search and discovery programmes. The discovery of novel natural products in marine microorganisms has increased linearly over the past two decades while those found in terrestrial microorganisms have remained almost unchanged over the same period [3].…”

Section: Introductionmentioning

confidence: 99%

Proximicin A, B and C, Novel Aminofuran Antibiotic and Anticancer Compounds Isolated from Marine Strains of the Actinomycete Verrucosispora†

et al. 2008

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A family of three novel aminofuran antibiotics named as proximicins was isolated from the marine Verrucosispora strain MG-37. Proximicin A was detected in parallel in the marine abyssomicin producer "Verrucosispora maris" AB-18-032. The characteristic structural element of proximicins is 4-amino-furan-2-carboxylic acid, a hitherto unknown g -amino acid. Proximicins show a weak antibacterial activity but a strong cytostatic effect to various human tumor cell lines.

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Marine actinobacteria: new opportunities for natural product search and discovery

Cited by 338 publications

References 72 publications

Analysis of bacterial and archaeal diversity in coastal microbial mats using massive parallel 16S rRNA gene tag sequencing

Analysis of bacterial and archaeal diversity in coastal microbial mats using massive parallel 16S rRNA gene tag sequencing

Analysis of Antarctic proteobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites

Proximicin A, B and C, Novel Aminofuran Antibiotic and Anticancer Compounds Isolated from Marine Strains of the Actinomycete Verrucosispora†

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