Marine bacterioplankton biomass, activity and community structure in the vicinity of Antarctic icebergs

Murray, Alison E.; Peng, Vivian; Tyler, Charlotte; Wagh, Protima

doi:10.1016/j.dsr2.2010.11.021

Cited by 17 publications

(17 citation statements)

References 56 publications

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“…The SAR86‐1249 probe detects 62% of the entire SAR86 clade (Supporting information http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12258/suppinfo). The few studies exploring bacterial diversity in WAP waters found only 13 SAR86 ribotypes in these waters (Murray and Grzymski, ; Murray et al ., ; Grzymski et al ., ), of which 69% are detected by the SAR86‐1249 probe (Supporting information http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12258/suppinfo). CARD FISH abundance of all bacterial (EUB338‐positive) cells increased significantly from 56.9 ± 10.9% in fall to 76.4 ± 7.3% in summer (Student's t ‐test, P < 0.05) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Growth activity of gammaproteobacterial subgroups in waters off the west Antarctic Peninsula in summer and fall

Nikrad

Cottrell

Kirchman

2013

Environmental Microbiology

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Characterizing both growth and abundance is important in understanding the role of bacterial communities in biogeochemical cycling of global oceans. However, these two quantities are seldom measured together for specific bacterial clades. Our goal was to examine growth and abundance of three gammaproteobacterial subgroups, including SAR86, at the single-cell level by microautoradiography combined with fluorescence in situ hybridization (FISH) in coastal waters of the west Antarctic Peninsula region during two austral summers and one austral fall. We found that the SAR86 clade was less abundant and grew more slowly than two related gammaproteobacterial clades, Ant4D3 and Arctic96B-16. Over 60% of Ant4D3 and Arctic96B-16 cells incorporated leucine, while only 25% of SAR86 cells were active in both summer and fall. We also explored using the size of the FISH image as another measure of single-cell activity. There was a linear relationship between FISH cell size and incorporation of leucine for all bacteria, Ant4D3 and Arctic96B-16, but not for SAR86. FISH sizes of SAR86 cells were at least threefold smaller than cells in the other clades. Our results suggest slow growth of SAR86 in the perennially cold waters of the west Antarctic Peninsula.

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Section: Resultsmentioning

confidence: 99%

Growth activity of gammaproteobacterial subgroups in waters off the west Antarctic Peninsula in summer and fall

Nikrad

Cottrell

Kirchman

2013

Environmental Microbiology

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“…In contrast, ~ 17% of excised 16S‐DGGE bands from summer Antarctic Peninsula waters matched Ant4D3, dominating the Gammaproteobacteria and outnumbering those from winter Antarctic Peninsula and from Kerguelen Island waters (Ghiglione & Murray, ). Ant4D3 sequences were also abundant in a 16S rRNA gene library from waters in the vicinity of Antarctic icebergs (Murray et al ., ). Little is known about the function and ecological role of Ant4D3, although it has been detected in Antarctic waters associated with a phytoplankton bloom (Williams et al ., ), and Arctic waters where it appeared to occupy a DOM utilization niche different from that of other major heterotrophs such as SAR11 (Nikrad et al ., ).…”

Section: The Southern Oceanmentioning

confidence: 97%

“…SAR11 has been consistently detected at high abundances in molecular surveys of the Southern Ocean. The surveys include open ocean regions as well as at depth and in coastal waters, and SAR11 is usually the dominant alphaproteobacterial, if not bacterial, group (García‐Martínez and Rodríguez‐Valera, ; López‐García et al ., ; Murray & Grzymski, ; Giebel et al ., ; Murray et al ., ; Straza et al ., ; Piquet et al ., ; Ghiglione & Murray, ; Jamieson et al ., ), and appears to be more abundant in the epipelagic zone than at depth (Giebel et al ., ).…”

Section: The Southern Oceanmentioning

confidence: 98%

Key microbial drivers in Antarctic aquatic environments

et al. 2013

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Antarctica is arguably the world's most important continent for influencing the Earth's climate and ocean ecosystem function. The unique physico-chemical properties of the Southern Ocean enable high levels of microbial primary production to occur. This not only forms the base of a significant fraction of the global oceanic food web, but leads to the sequestration of anthropogenic CO2 and its transport to marine sediments, thereby removing it from the atmosphere; the Southern Ocean accounts for ~ 30% of global ocean uptake of CO2 despite representing ~ 10% of the total surface area of the global ocean. The Antarctic continent itself harbors some liquid water, including a remarkably diverse range of surface and subglacial lakes. Being one of the remaining natural frontiers, Antarctica delivers the paradox of needing to be protected from disturbance while requiring scientific endeavor to discover what is indigenous and learn how best to protect it. Moreover, like many natural environments on Earth, in Antarctica, microorganisms dominate the genetic pool and biomass of the colonizable niches and play the key roles in maintaining proper ecosystem function. This review puts into perspective insight that has been and can be gained about Antarctica's aquatic microbiota using molecular biology, and in particular, metagenomic approaches.

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“…Cultivation-independent molecular techniques have been used in an attempt to examine the composition of bacterial communities in the Antarctic Ocean (Kim et al, 2013;Murray et al, 2011;Straza et al, 2010). Recently, meta-omics (metagenomics, metaproteomics and metatranscriptomics) studies have provided new insight into the functional roles of heterotrophic bacteria associated with austral phytoplankton blooms (Grzymski et al, 2012;Wilkins et al, 2013;Williams et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Cultivation and biochemical characterization of heterotrophic bacteria associated with phytoplankton bloom in the Amundsen sea polynya, Antarctica

Choi

Kim

Jung

et al. 2016

Deep Sea Research Part II: Topical Studies in Oceanography

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Marine bacterioplankton biomass, activity and community structure in the vicinity of Antarctic icebergs

Cited by 17 publications

References 56 publications

Growth activity of gammaproteobacterial subgroups in waters off the west Antarctic Peninsula in summer and fall

Growth activity of gammaproteobacterial subgroups in waters off the west Antarctic Peninsula in summer and fall

Key microbial drivers in Antarctic aquatic environments

Cultivation and biochemical characterization of heterotrophic bacteria associated with phytoplankton bloom in the Amundsen sea polynya, Antarctica

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