Marine biosurfactants. IV. Production, characterization and biosynthesis of an anionic glucose lipid from the marine bacterial strain MM1

Passeri, A.; Schmidt, Michael; Haffner, Thomas; Wray, Victor; Lang, Siegmund; Wagner, Fritz

doi:10.1007/bf00210978

Cited by 68 publications

(48 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A microbial cell that is able to excrete biosurfactant into the aqueous phase may be responsible for the formation of a conditioning film at the interphase. Other studies showed that, e.g., glycolipids reduce the surface tension [57]. Thereby the hydrophobicity of the cells increases and concomittant with this their affinity for oil droplets suspended in the water phase [23].…”

Section: Discussionmentioning

confidence: 98%

Cell surface analysis of the lipid‐discharging obligate hydrocarbonoclastic species of the genus Alcanivorax

Lange

Tenberge²,

Robenek

et al. 2010

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

This study presents novel information useful for addressing the question how species of the genus Alcanivorax discharge triacylglycerols (TAG) and/or wax esters (WE). The observed structures were referred as ''blebs'' according to Gauthier et al. [1] to avoid confusion with other discharging phenomena. The cells were aerobically cultivated on solid media and not in liquid media to maintain the cells in the native state, and were investigated by transmission electron microscopic (TEM) and scanning electron microscopic (SEM) methods to document the surface structures of the cells. The phenomenon of lipid export could be allocated to three phases: phase I: protrusion formation of the cell membrane occurred; phase II: discharging progressed further with blebs becoming larger; and phase III: the blebs at the cell surface were separated from the cells. Using freeze-fracture micrographs by TEM, vesicle experiments and TLC, we have shown that the blebs contained TAG and WE. The results shown in this study will support further research to unravel the unknown discharging mechanism. In addition, the formation of an extensive extracellular matrix was observed by SEM.

show abstract

Section: Discussionmentioning

confidence: 98%

Cell surface analysis of the lipid‐discharging obligate hydrocarbonoclastic species of the genus Alcanivorax

Lange

Tenberge²,

Robenek

et al. 2010

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

show abstract

“…A. borkumensis forms stable emulsions of hydrocarbon in water and produces biosurfactants 23 . These biosurfactants are anionic glucolipids carrying four fatty acids of varying chain lengths 11 .…”

Section: Emulsification Of Hydrocarbons By a Borkumensis Sk2mentioning

confidence: 99%

Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis

et al. 2006

Self Cite

View full text Add to dashboard Cite

Alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. Although barely detectable in unpolluted environments, A. borkumensis becomes the dominant microbe in oil-polluted waters. A. borkumensis SK2 has a streamlined genome with a paucity of mobile genetic elements and energy generation–related genes, but with a plethora of genes accounting for its wide hydrocarbon substrate range and efficient oil-degradation capabilities. The genome further specifies systems for scavenging of nutrients, particularly organic and inorganic nitrogen and oligo-elements, biofilm formation at the oil-water interface, biosurfactant production and niche-specific stress responses. The unique combination of these features provides A. borkumensis SK2 with a competitive edge in oil-polluted environments. This genome sequence provides the basis for the future design of strategies to mitigate the ecological damage caused by oil spills. Supplementary information The online version of this article (doi:10.1038/nbt1232) contains supplementary material, which is available to authorized users.

show abstract

“…For HC-source Petri dish incubations, a filter-paper of the appropriate size was soaked with 1-2 mL of the substrate (n-C 12 or crude oil) and placed in the lid. The bottom dish contained agar (15 g L À1 ) mixed in mineral medium (Passeri et al, 1992). The mineral medium agar plates were inoculated with the pure bacterial culture, placed onto the dish lid and incubated in the "bottom-up-lid-down"position.…”

Section: Solid Medium Incubationsmentioning

confidence: 99%

Fatty acids in bacterium Dietzia sp. grown on simple and complex hydrocarbons determined as FAME by GC–MS.

Hvidsten

Mjøs

Bødtker

et al. 2015

Chemistry and Physics of Lipids

View full text Add to dashboard Cite

Marine biosurfactants. IV. Production, characterization and biosynthesis of an anionic glucose lipid from the marine bacterial strain MM1

Cited by 68 publications

References 20 publications

Cell surface analysis of the lipid‐discharging obligate hydrocarbonoclastic species of the genus Alcanivorax

Cell surface analysis of the lipid‐discharging obligate hydrocarbonoclastic species of the genus Alcanivorax

Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis

Fatty acids in bacterium Dietzia sp. grown on simple and complex hydrocarbons determined as FAME by GC–MS.

Contact Info

Product

Resources

About