Marine fungal DHICA as a UVB protectant: Assessment under in vitro and in vivo conditions

Shanuja, S.K.; Iswarya, S.; Gnanamani, A.

doi:10.1016/j.jphotobiol.2018.01.010

Cited by 17 publications

(13 citation statements)

References 55 publications

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“…Among these marine-derived strains, Aspergillus nidulans, an anamorph of Emericella nidulans, is an important model ascomycete for eukaryotic genetics. A few studies have been dedicated to marine-derived A. nidulans species, such as two relatively recent ones reporting on the production of molecules of interest: melanin precursors with UVB protective properties [24] and antitumor alkaloids [25]. Another strain identified in this study belongs to the phylum ascomycetes (Dothideomycetes, Pleosporales, Pleosporaceae), specifically to the Stemphylium genus, that encompasses worldwide-distributed saprophytes and plant pathogens affecting a variety of agricultural crops.…”

Section: Discussionmentioning

confidence: 99%

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications

et al. 2020

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Background: Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxidoreductases). Oxidative enzymes from microorganisms offer eco-friendly, cost-effective processes amenable to biotechnological applications, such as in industrial dye decolorization. The aim of this study was to screen marinederived fungal strains isolated from three coastal areas in Tunisia to identify laccase-like activities, and to produce and characterize active cell-free supernatants of interest for dye decolorization. Results: Following the screening of 20 fungal strains isolated from the harbors of Sfax and Monastir (Tunisia), five strains were identified that displayed laccase-like activities. Molecular-based taxonomic approaches identified these strains as belonging to the species Trichoderma asperellum, Stemphylium lucomagnoense and Aspergillus nidulans. Among these five isolates, one T. asperellum strain (T. asperellum 1) gave the highest level of secreted oxidative activities, and so was chosen for further studies. Optimization of the growth medium for liquid cultures was first undertaken to improve the level of laccase-like activity in culture supernatants. Finally, the culture supernatant of T. asperellum 1 decolorized different synthetic dyes belonging to diverse dye families, in the presence or absence of 1hydroxybenzotriazole (HBT) as a mediator. Conclusions: The optimal growth conditions to produce laccase-like active cell-free supernatants from T. asperellum 1 were 1.8 mM CuSO 4 as an inducer, 1% NaCl to mimic a seawater environment and 3% sucrose as a carbon source. The culture supernatant of T. asperellum 1 effectively decolorized different synthetic dyes belonging to diverse chemical classes, and the presence of HBT as a mediator improved the decolorization process.

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Section: Discussionmentioning

confidence: 99%

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications

et al. 2020

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“…UVB frequently causes mutations in the p53 gene, which is a faulty trigger of apoptosis in keratinocytes and promotes non-melanoma skin cancers 7 . The previous study also reported that DNA damage induces apoptosis through the tumor suppressor protein, p53, which in turn causes cell cycle arrest, while triggering cellular repair systems 8 . Moreover, recent studies have indicated that UVB exposure can cause the dysfunction of skin epidermal barrier and skin dryness and finally skin aging and cancers 4 , 9 .…”

Section: Introductionmentioning

confidence: 88%

UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism

Han

Kim

Han

et al. 2021

Sci Rep

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The present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratinocytes and its underlying mechanism. SHM significantly improved cell viability of UVB-exposed human keratinocytes by reducing the generation of intracellular reactive oxygen species (ROS). Moreover, SHM inhibited UVB exposure-induced apoptosis by reducing the formation of apoptotic bodies and the populations of the sub-G1 hypodiploid cells and the early apoptotic cells by modulating the expression of the anti- and pro-apoptotic molecules, Bcl-2 and Bax, respectively. Furthermore, SHM inhibited NF-κB p65 activation by inducing the activation of Nrf2/HO-1 signaling. The cytoprotective and antiapoptotic activities of SHM are abolished by the inhibition of HO-1 signaling. In further study, SHM restored the skin dryness and skin barrier disruption in UVB-exposed human keratinocytes. Based to these results, our study suggests that SHM protects the cells against UVB-induced cellular damages through the Nrf2/HO-1/NF-κB p65 signaling pathway and may be potentially useful for the prevention of UVB-induced skin damage.

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“…Among these marine-derived strains, Aspergillus nidulans, an anamorph of Emericella nidulans, is an important model ascomycete for eukaryotic genetics. A few studies have been dedicated to marine-derived A. nidulans species, such as two relatively recent ones reporting on the production of molecules of interest: melanin precursors with UVB protective properties [23] and antitumor alkaloids [24]. Another strain identified in this study belongs to the phylum ascomycetes (Dothideomycetes, Pleosporales, Pleosporaceae), specifically to the Stemphylium genus, that encompasses worldwide-distributed saprophytes and plant pathogens affecting a variety of agricultural crops.…”

Section: Discussionmentioning

confidence: 99%

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications

Ali

Chaduli

Navarro

et al. 2020

Preprint

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Background : Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxidoreductases). Oxidative enzymes from microorganisms offer eco-friendly, cost–effective processes amenable to biotechnological applications, such as in industrial dye decolorization. The aim of this study was to screen marine-derived fungal strains isolated from three coastal areas in Tunisia to identify laccase-like activities, and to produce and characterize active cell-free supernatants of interest for dye decolorization.Results : Following the screening of 20 fungal strains isolated from the harbors of Sfax and Monastir (Tunisia), five strains were identified that displayed laccase-like activities. Molecular-based taxonomic approaches identified these strains as belonging to the species Trichoderma asperellum , Stemphylium lucomagnoense and Aspergillus nidulans . Among these five isolates, one T. asperellum strain ( T. asperellum 1) gave the highest level of secreted oxidative activities, and so was chosen for further studies. Optimization of the growth medium for liquid cultures was first undertaken to improve the level of laccase-like activity in culture supernatants. Finally, the culture supernatant of T. asperellum 1 decolorized different synthetic dyes belonging to diverse dye families, in the presence or absence of 1-hydroxybenzotriazole (HBT) as a mediator.Conclusions : The optimal growth conditions to produce laccase-like active cell-free supernatants from T. asperellum 1 were 1.8 mM CuSO 4 as an inducer, 1% NaCl to mimic a seawater environment and 3% sucrose as a carbon source. The culture supernatant of T. asperellum 1 effectively decolorized different synthetic dyes belonging to diverse chemical classes, and the presence of HBT as a mediator improved the decolorization process.

show abstract

Marine fungal DHICA as a UVB protectant: Assessment under in vitro and in vivo conditions

Cited by 17 publications

References 55 publications

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications

UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications

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