Marine fungi in the spotlight: opportunities and challenges for marine fungal natural product discovery and biotechnology

Taşdemir, Deniz

doi:10.1186/s40694-017-0034-1

Cited by 11 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cultivability remains one of the main limitations associated with the discovery of natural products from marine fungi s.s. [5]. Isolation techniques commonly reported in the literature by marine natural products groups are dominated by simple plating methods, approaches that favor the isolation of faster growing generalist, osmotolerant/halotolerant fungal genera.…”

Section: Introductionmentioning

confidence: 99%

The Neglected Marine Fungi, Sensu stricto, and Their Isolation for Natural Products’ Discovery

et al. 2019

View full text Add to dashboard Cite

Despite the rapid development of molecular techniques relevant for natural product research, culture isolates remain the primary source from which natural products chemists discover and obtain new molecules from microbial sources. Techniques for obtaining and identifying microbial isolates (such as filamentous fungi) are thus of crucial importance for a successful natural products’ discovery program. This review is presented as a “best-practices guide” to the collection and isolation of marine fungi for natural products research. Many of these practices are proven techniques used by mycologists for the isolation of a broad diversity of fungi, while others, such as the construction of marine baiting stations and the collection and processing of sea foam using dilution to extinction plating techniques, are methodological adaptations for specialized use in marine/aquatic environments. To this day, marine fungi, Sensu stricto, remain one of the few underexplored resources of natural products. Cultivability is one of the main limitations hindering the discovery of natural products from marine fungi. Through encouraged collaboration with marine mycologists and the sharing of historically proven mycological practices for the isolation of marine fungi, our goal is to provide natural products chemists with the necessary tools to explore this resource in-depth and discover new and potentially novel natural products.

show abstract

Section: Introductionmentioning

confidence: 99%

The Neglected Marine Fungi, Sensu stricto, and Their Isolation for Natural Products’ Discovery

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Fungi are an understudied resource for the discovery of novel natural products [ 4 , 11 , 16 , 18 , 49 , 50 , 51 ]. Marine fungi account for more than 60% of the 456 newly reported marine microbial natural products [ 52 ], an indication of the huge scale of untapped biosynthetic potential of naturally occurring fungi.…”

Section: Discussionmentioning

confidence: 99%

Diversity and Biosynthetic Potential of Fungi Isolated from St. John’s Island, Singapore

Munusamy

Tan

Nge

et al. 2023

IJMS

View full text Add to dashboard Cite

Adaptation to a wide variety of habitats allows fungi to develop unique abilities to produce diverse secondary metabolites with diverse bioactivities. In this study, 30 Ascomycetes fungi isolated from St. John’s Island, Singapore were investigated for their general biosynthetic potential and their ability to produce antimicrobial secondary metabolites (SMs). All the 30 fungal isolates belong to the Phylum Ascomycota and are distributed into 6 orders and 18 genera with Order Hypocreales having the highest number of representative (37%). Screening for polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) genes using degenerate PCR led to the identification of 23 polyketide synthases (PKSs) and 5 nonribosomal peptide synthetases (NRPSs) grouped into nine distinct clades based on their reduction capabilities. Some of the identified PKSs genes share high similarities between species and known reference genes, suggesting the possibility of conserved biosynthesis of closely related compounds from different fungi. Fungal extracts were tested for their antimicrobial activity against S. aureus, Methicillin-resistant S. aureus (MRSA), and Candida albicans. Bioassay-guided fractionation of the active constituents from two promising isolates resulted in the isolation of seven compounds: Penilumamides A, D, and E from strain F4335 and xanthomegnin, viomellein, pretrichodermamide C and vioxanthin from strain F7180. Vioxanthin exhibited the best antibacterial activity with IC50 values of 3.0 μM and 1.6 μM against S. aureus and MRSA respectively. Viomellein revealed weak antiproliferative activity against A549 cells with an IC50 of 42 μM. The results from this study give valuable insights into the diversity and biosynthetic potential of fungi from this unique habitat and forms a background for an in-depth analysis of the biosynthetic capability of selected strains of interest with the aim of discovering novel fungal natural products.

show abstract

“…The only bioactive fungal strain A. pullulans is a member of the family Saccotheciaceae (class Dothideomycetes, phylum Ascomycota). Phylum Ascomycota is well known as a rich source of complex and highly active natural products [89,90]. The solvent extracts of A. pullulans displayed bioactivity against the gram-positive pathogens E. faecium, MRSA and the fish pathogen L. garviae, when it was cultured on agar-based medium.…”

Section: Discussionmentioning

confidence: 99%

Gut and Gill-Associated Microbiota of the Flatfish European Plaice (Pleuronectes platessa): Diversity, Metabolome and Bioactivity against Human and Aquaculture Pathogens

et al. 2022

Self Cite

View full text Add to dashboard Cite

Similar to other marine holobionts, fish are colonized by complex microbial communities that promote their health and growth. Fish-associated microbiota is emerging as a promising source of bioactive metabolites. Pleuronectes platessa (European plaice, plaice), a flatfish with commercial importance, is common in the Baltic Sea. Here we used a culture-dependent survey followed by molecular identification to identify microbiota associated with the gills and the gastrointestinal tract (GIT) of P. platessa, then profiled their antimicrobial activity and metabolome. Altogether, 66 strains (59 bacteria and 7 fungi) were isolated, with Proteobacteria being the most abundant phylum. Gill-associated microbiota accounted for higher number of isolates and were dominated by the Proteobacteria (family Moraxellaceae) and Actinobacteria (family Nocardiaceae), whereas Gram-negative bacterial families Vibrionaceae and Shewanellaceae represented the largest group associated with the GIT. The EtOAc extracts of the solid and liquid media cultures of 21 bacteria and 2 fungi representing the diversity of cultivable plaice-associated microbiota was profiled for their antimicrobial activity against three fish pathogens, human bacterial pathogen panel (ESKAPE) and two human fungal pathogens. More than half of all tested microorganisms, particularly those originating from the GIT epithelium, exhibited antagonistic effect against fish pathogens (Lactococcus garvieae, Vibrio ichthyoenteri) and/or human pathogens (Enterococcus faecium, methicillin-resistant Staphylococcus aureus). Proteobacteria represented the most active isolates. Notably, the solid media extracts displayed higher activity against fish pathogens, while liquid culture extracts were more active against human pathogens. Untargeted metabolomics approach using feature-based molecular networking showed the high chemical diversity of the liquid extracts that contained undescribed clusters. This study highlights plaice-associated microbiota as a potential source of antimicrobials for the control of human and the aquaculture-associated infections. This is the first study reporting diversity, bioactivity and chemical profile of culture-dependent microbiota of plaice.

show abstract

Marine fungi in the spotlight: opportunities and challenges for marine fungal natural product discovery and biotechnology

Cited by 11 publications

References 9 publications

The Neglected Marine Fungi, Sensu stricto, and Their Isolation for Natural Products’ Discovery

The Neglected Marine Fungi, Sensu stricto, and Their Isolation for Natural Products’ Discovery

Diversity and Biosynthetic Potential of Fungi Isolated from St. John’s Island, Singapore

Gut and Gill-Associated Microbiota of the Flatfish European Plaice (Pleuronectes platessa): Diversity, Metabolome and Bioactivity against Human and Aquaculture Pathogens

Contact Info

Product

Resources

About