Marine Fungus Aspergillus chevalieri TM2-S6 Extract Protects Skin Fibroblasts from Oxidative Stress

Letsiou, Sophia; Bakea, Artemis; Goff, Géraldine Le; Lopes, Philippe; Gardikis, Κonstantinos; Weis, Michal; Benayahu, Yehuda; Ouazzani, Jamal

doi:10.3390/md18090460

Cited by 15 publications

(15 citation statements)

References 56 publications

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“…RNA isolation, cDNA synthesis, as well as RT-qPCR method analysis were carried out as previously outlined [17][18][19]. Briefly, for RNA isolation (500 ng), the Nucleospin RNA kit (Macherey-Nagel, Germany) was used, and for cDNA (reaction volume of 20 µL), the SuperScript™ First-Strand Synthesis kit (Invitrogen, UK) was used.…”

Section: Rt-qpcr Gene Expression Analysismentioning

confidence: 99%

Exploring the Protective Effects of Phaeodactylum tricornutum Extract on LPS-Treated Fibroblasts

Mosxou

Letsiou

2021

Cosmetics

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Background: Microalgal extracts are an important source of bioactive compounds with antioxidant and anti-inflammatory properties that can be used in cosmetics. The microalgae Phaeodactylum tricornutum (PT) is known for its high content of omega-3 fatty acids, which are known to attenuate inflammation. Here, we explore the effects of aqueous microencapsulated extract of PT on lipopolysaccharide (LPS)-stimulated normal human dermal fibroblasts (NHDF) to underline its application as an active ingredient in cosmetics. Methods: We assessed cell viability using MTT assay, so as to target any potential cytotoxicity of the extract. Moreover, with the aid of RT-qPCR, we studied the transcript accumulation of genes involved in cell antioxidant response, cell proliferation, and inflammation. Results: Our results revealed that the hydrolyzed rice flour-encapsulated (HRF) PT extract promotes anti-inflammatory and antioxidant response, increasing cell proliferation in NHDF cells. Conclusions: Our data indicate a promising use of HRF-encapsulated PT extract in cosmetics by reducing skin inflammation.

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Section: Rt-qpcr Gene Expression Analysismentioning

confidence: 99%

Exploring the Protective Effects of Phaeodactylum tricornutum Extract on LPS-Treated Fibroblasts

Mosxou

Letsiou

2021

Cosmetics

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“…The anti-oxidant and anti-aging properties of the culture filtrate (ACCB) obtained from the sponge-associated Aspergillus chavalieri TM2-S6 (Tel Aviv-Jaffa, Israeli Mediterranean coast) were also tested on primary normal human dermal fibroblasts (NHDF) through bioassays and molecular approaches [ 115 ]. Chemical analyses on the ACCB ethyl acetate extract revealed two abundant compounds, named Tetrahydroauroglaucin and Flavoglaucin.…”

Section: Sponge Symbionts In Cosmeceutical Fieldmentioning

confidence: 99%

“…Interestingly, the mRNA levels of sirtuin 1 ( SIRT1 ) and sirtuin 2 ( SIRT2 ), implicated in skin aging, were also found up-regulated in H 2 O 2 -induced NHDF cells. Combining these results, the authors concluded that ACCB stimulated cell proliferation, anti-oxidant response and extracellular matrix organization as well as reduced aging, thus proposing ACCB as a perfect candidate for the formulation of cosmetic products [ 115 ].…”

Section: Sponge Symbionts In Cosmeceutical Fieldmentioning

confidence: 99%

Sponges and Their Symbionts as a Source of Valuable Compounds in Cosmeceutical Field

et al. 2021

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In the last decades, the marine environment was discovered as a huge reservoir of novel bioactive compounds, useful for medicinal treatments improving human health and well-being. Among several marine organisms exhibiting biotechnological potential, sponges were highlighted as one of the most interesting phyla according to a wide literature describing new molecules every year. Not surprisingly, the first marine drugs approved for medical purposes were isolated from a marine sponge and are now used as anti-cancer and anti-viral agents. In most cases, experimental evidence reported that very often associated and/or symbiotic communities produced these bioactive compounds for a mutual benefit. Nowadays, beauty treatments are formulated taking advantage of the beneficial properties exerted by marine novel compounds. In fact, several biological activities suitable for cosmetic treatments were recorded, such as anti-oxidant, anti-aging, skin whitening, and emulsifying activities, among others. Here, we collected and discussed several scientific contributions reporting the cosmeceutical potential of marine sponge symbionts, which were exclusively represented by fungi and bacteria. Bioactive compounds specifically indicated as products of the sponge metabolism were also included. However, the origin of sponge metabolites is dubious, and the role of the associated biota cannot be excluded, considering that the isolation of symbionts represents a hard challenge due to their uncultivable features.

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“…Since the first report in 1934 of FG and DAG from the terrestrial fungus Aspergillus glaucus [ 7 ], this growing class of C7 alkylated salicylaldehyde derivatives [ 8 ] has been described to possess a broad spectrum of biological activities: antioxidant and radical scavenging properties [ 9 , 10 , 11 ], antimicrobial, antibiofilm [ 12 ], and antifouling activities [ 3 ], anti-inflammatory [ 13 , 14 ] and skin-protective properties under oxidative stress condition [ 15 ] as well as binding affinity to human opioid or cannabinoid receptors [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Dihydroauroglaucin Isolated from the Mediterranean Sponge Grantia compressa Endophyte Marine Fungus Eurotium chevalieri Inhibits Migration of Human Neuroblastoma Cells

et al. 2022

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Cancer cell migration is a hallmark of the aggressiveness and progression of malignancies such as high-risk neuroblastoma. Given the lack of effective therapeutic solutions to counteract cancer progression, basic research aims to identify novel bioactive molecules with inhibitory potential on cancer cell migration. In this context, this work investigated the role of members of the salicylaldehyde secondary metabolite set from the sponge endophyte fungus Eurotium chevalieri MUT 2316 as potential inhibitors of human neuroblastoma SH-SY5Y cell migration. Since tetrahydroauroglaucin (TAG) and dihydroauroglaucin (DAG) were isolated in large amounts, both were evaluated for their anticancer properties towards SH-SY5Y cells. Both molecules were found to be non-cytotoxic by MTT assay and cytofluorimetric analysis. Moreover, DAG showed efficacy in inhibiting the highly migratory phenotype of SH-SY5Y cells by wound healing assay; whereas TAG, although structurally similar to DAG, showed no anti-migratory effect. Therefore, this work provides good reasons to conduct further in vitro and in vivo studies focusing on DAG as a potentially useful migrastatic natural marine molecule.

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Marine Fungus Aspergillus chevalieri TM2-S6 Extract Protects Skin Fibroblasts from Oxidative Stress

Cited by 15 publications

References 56 publications

Exploring the Protective Effects of Phaeodactylum tricornutum Extract on LPS-Treated Fibroblasts

Exploring the Protective Effects of Phaeodactylum tricornutum Extract on LPS-Treated Fibroblasts

Sponges and Their Symbionts as a Source of Valuable Compounds in Cosmeceutical Field

Dihydroauroglaucin Isolated from the Mediterranean Sponge Grantia compressa Endophyte Marine Fungus Eurotium chevalieri Inhibits Migration of Human Neuroblastoma Cells

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