Marine Microbial Enzymes: Biotechnological and Biomedical Aspects

Sana, Barindra

doi:10.1002/9783527665259.ch26

Cited by 3 publications

(1 citation statement)

References 67 publications

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“…Microrganismos extremófilos e microrganismos simbióticos (arqueias e bactérias marinhas, como Vibrio, Bacillus, Pseudoalteromonas, Pseudomonas e Staphylothermus, além de actinobactérias e fungos) são fontes da maioria das enzimas marinhas com propriedades químicas e perfil de atividades biocatalíticas inéditas. Algumas dessas vêm sendo pesquisadas e até utilizadas em setores industriais, como alimentício e de detergentes (Trincone, 2011;Sana, 2013;Parages et al, 2016). O registro de patentes de enzimas hidrolíticas derivadas de microrganismos marinhos vêm aumentando nos últimos anos, conforme listado na Tab.…”

Section: Enzimas Microbianas Marinhasunclassified

Microrganismos marinhos: um reservatório de hidrolases biotecnologicamente interessantes

Lopes,

Canellas,

Oliveira

et al. 2023

Rev. Biol.

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Esta revisão tem por objetivo fornecer uma revisão narrativa atualizada acerca de enzimas hidrolíticas (agarases, amilases, celulases, esterases, lipases, peptidases e quitinases) isoladas do microbioma marinho. Esses biocatalisadores apresentam propriedades bioquímicas únicas, como halotolerância, extremos de pH, temperatura e barofilicidade, que os tornam atraentes para uso em diversos setores industriais, estimulando futuras aplicações biotecnológicas. Considerando o vasto repertório enzimático dos diversos membros das comunidades microbianas vivendo no ambiente marinho, salienta-se a imprescindibilidade de acessar esse habitat, mais especificamente essas unidades biocatalíticas. Com a devida atenção voltada ao viés industrial, novos biocatalisadores serão descobertos com sucesso nas comunidades microbianas marinhas, desde a confirmação de sua atividade até a comprovação de seu emprego prático nos setores de interesse e geração de propriedade intelectual.

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Section: Enzimas Microbianas Marinhasunclassified

Microrganismos marinhos: um reservatório de hidrolases biotecnologicamente interessantes

Lopes,

Canellas,

Oliveira

et al. 2023

Rev. Biol.

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Perspectives for Novel Enzyme Discovery from Marine Environments through Genome Mining and Metagenomics

Bramhachari

Sheela

Satish

2020

Encyclopedia of Marine Biotechnology

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Characterization and diversity of the complete set of GH family 3 enzymes from Rhodothermus marinus DSM 4253

Ara

Månberger

Gabriško

et al. 2020

Sci Rep

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the genome of Rhodothermus marinus DSM 4253 encodes six glycoside hydrolases (GH) classified under GH family 3 (GH3): RmBgl3A, RmBgl3B, RmBgl3C, RmXyl3A, RmXyl3B and RmNag3. The biochemical function, modelled 3D-structure, gene cluster and evolutionary relationships of each of these enzymes were studied. The six enzymes were clustered into three major evolutionary lineages of GH3: β-N-acetyl-glucosaminidases, β-1,4-glucosidases/β-xylosidases and macrolide β-glucosidases. the RmNag3 with additional β-lactamase domain clustered with the deepest rooted GH3-lineage of β-N-acetyl-glucosaminidases and was active on acetyl-chitooligosaccharides. RmBgl3B displayed β-1,4-glucosidase activity and was the only representative of the lineage clustered with macrolide β-glucosidases from Actinomycetes. the β-xylosidases, RmXyl3A and RmXyl3B, and the β-glucosidases RmBgl3A and RmBgl3C clustered within the major β-glucosidases/β-xylosidases evolutionary lineage. RmXyl3A and RmXyl3B showed β-xylosidase activity with different specificities for para-nitrophenyl (pNP)-linked substrates and xylooligosaccharides. RmBgl3A displayed β-1,4-glucosidase/β-xylosidase activity while RmBgl3C was active on pnp-β-Glc and β-1,3-1,4-linked glucosyl disaccharides. Putative polysaccharide utilization gene clusters were also investigated for both R. marinus DSM 4253 and DSM 4252 t (homolog strain). The analysis showed that in the homolog strain DSM 4252 t Rmar_1080 (RmXyl3A) and Rmar_1081 (RmXyl3B) are parts of a putative polysaccharide utilization locus (PUL) for xylan utilization. Marine extremophilic biotopes, such as hot springs and hydrothermal vents, harbour diverse microbes hitherto underexploited and unexplored. Recent genomic studies show that many of the species, especially those found in coastal geothermal areas surrounded by profusion of carbohydrate rich biomass (seaweeds as well as terrestrial species), contain a wide array of novel glycoside hydrolases (GHs) 1,2. Thermostable GHs have numerous applications in different fields, making marine thermophiles targets for prospecting of industrially interesting enzymes 3,4. Rhodothermus marinus are Gram-negative marine thermophilic bacteria, previously classified under the phylum Bacteroidetes, but recently assigned to the new phylum Rhodothermaeota 5. The type-species was isolated from a coastal hot spring on the NorthWest coast of Iceland and has an optimum temperature of 65 °C and is slightly halophilic 6. R. marinus can utilize a variety of sugars as carbon sources and produces a wide range of GHs 6-12. Sequence analysis shows that the R. marinus genome contains a large number of genes encoding GH enzymes, many of which are secreted extracellularly; yet, several of them appear to be attached to the cell surface 13. These putative enzymes include six members of GH family 3 (GH3) (RmBgl3A, RmBgl3B, RmBgl3C, RmXyl3A, RmXyl3B and RmNag3), which have not yet been studied.

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Marine Microbial Enzymes: Biotechnological and Biomedical Aspects

Cited by 3 publications

References 67 publications

Microrganismos marinhos: um reservatório de hidrolases biotecnologicamente interessantes

Microrganismos marinhos: um reservatório de hidrolases biotecnologicamente interessantes

Perspectives for Novel Enzyme Discovery from Marine Environments through Genome Mining and Metagenomics

Characterization and diversity of the complete set of GH family 3 enzymes from Rhodothermus marinus DSM 4253

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