Marine PET Hydrolase (PET2): Assessment of Terephthalate- and Indole-Based Polyester Depolymerization

Abstract

Enzymatic polyethylene terephthalate (PET) recycling processes are gaining interest for their low environmental impact, use of mild conditions, and specificity. Furthermore, PET hydrolase enzymes are continuously being discovered and engineered. In this work, we studied a PET hydrolase (PET2), initially characterized as an alkaline thermostable lipase. PET2 was produced in a fusion form with a 6-histidine tag in the N-terminal. The PET2 activity on aromatic terephthalate and new indole-based polyesters was evaluated using polymers in powder form. Compared with IsPETase, an enzyme derived from Ideonella sakaiensis, PET2 showed a lower PET depolymerization yield. However, interestingly, PET2 produced significantly higher polybutylene terephthalate (PBT) and polyhexylene terephthalate (PHT) depolymerization yields. A clear preference was found for aromatic indole-derived polyesters over non-aromatic ones. No activity was detected on Akestra™, an amorphous copolyester with spiroacetal structures. Docking studies suggest that a narrower and more hydrophobic active site reduces its activity on PET but favors its interaction with PBT and PHT. Understanding the enzyme preferences of polymers will contribute to their effective use to depolymerize different types of polyesters.

Acinetobacter guillouiaestrain isolated from sludge capable of partially degrade polyethylene terephthalate: genomic and biochemical insights

“…Then, a specialized transporter in the periplasm imports the TPA into the cytoplasm (Yoshida et al, 2016). MHET is also the main product when marine PET2 (Wagner-Egea et al, 2023) and leaf-branch compost cutinase (LCC) from bacterial origin are used for TPA depolymerization (Tournier et al, 2020). The extracellular enzymatic extract from Stenotrophomonas maltophilia also yields MEHT as the major PET powder degradation product (Din et al, 2023).…”

“…PET Ramapet N180 was purchased from Indorama and prepared as a semicrystalline powder by solubilisation and precipitation, as described before (Wagner-Egea et al, 2021). The crystallinity was 11.3%, determined previously by Differential Scanning Calorimetry(Wagner-Egea et al, 2023).…”