Marine protease-producing bacterium and its potential use as an abalone probiont

Amin, Muhamad

doi:10.1016/j.aqrep.2018.09.004

Cited by 28 publications

(19 citation statements)

References 26 publications

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“…As we all know, peptides and amino acids are essential for subsequent catabolism of organisms. Reportedly, protease-producing bacteria could not only improve protein digestibility and growth of the host, but also reduce organic pollutants in aquaculture ( Shi et al, 2016 ; Amin, 2018 ; Su et al, 2020 ). Protease-producing bacteria that belong to four major phyla, Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes, have been identified, which are dominated by Bacillus , Pseudomonas , and Pseudoalteromonas genera ( Su et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…During the feed processing, dietary enzymes may lose their activities under high temperature and pressure conditions. Fortunately, an increasingly accepted method by supplementing protease-producing bacteria considered as an environment-friendly method was good for growth enhancement ( Shi et al, 2016 ; Amin, 2018 ), which has been confirmed to not only enhance protein digestibility, but also reduce organic pollutants produced by undigested feed ( Suzer et al, 2008 ; Ambas et al, 2015 ; Reda and Selim, 2015 ). Reportedly, feed by supplementing 1.0 × 10 10 CFU/g protease-producing bacteria could improve growth and nonspecific immune system of Nile tilapia ( Selim and Reda, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Community Structure of Protease-Producing Bacteria Cultivated From Aquaculture Systems: Potential Impact of a Tropical Environment

Wei

Long

et al. 2021

Front. Microbiol.

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Protease-producing bacteria play vital roles in degrading organic matter of aquaculture system, while the knowledge of diversity and bacterial community structure of protease-producing bacteria is limited in this system, especially in the tropical region. Herein, 1,179 cultivable protease-producing bacterial strains that belonged to Actinobacteria, Firmicutes, and Proteobacteria were isolated from tropical aquaculture systems, of which the most abundant genus was Bacillus, followed by Vibrio. The diversity and relative abundance of protease-producing bacteria in sediment were generally higher than those in water. Twenty-one genera from sediment and 16 genera from water were identified, of which Bacillus dominated by Bacillus hwajinpoensis in both and Vibrio dominated by Vibrio owensii in water were the dominant genera. The unique genera in sediment or water accounted for tiny percentage may play important roles in the stability of community structure. Eighty V. owensii isolates were clustered into four clusters (ET-1–ET-4) at 58% of similarity by ERIC-PCR (enterobacterial repetitive intergenic consensus-polymerase chain reaction), which was identified as a novel branch of V. owensii. Additionally, V. owensii strains belonged to ET-3 and ET-4 were detected in most aquaculture ponds without outbreak of epidemics, indicating that these protease-producing bacteria may be used as potential beneficial bacteria for wastewater purification. Environmental variables played important roles in shaping protease-producing bacterial diversity and community structure in aquaculture systems. In sediment, dissolved oxygen (DO), chemical oxygen demand (COD), and salinity as the main factors positively affected the distributions of dominant genus (Vibrio) and unique genera (Planococcus and Psychrobacter), whereas temperature negatively affected that of Bacillus (except B. hwajinpoensis). In water, Alteromonas as unique genus and Photobacterium were negatively affected by NO3−-N and NO2−-N, respectively, whereas pH as the main factor positively affected the distribution of Photobacterium. These findings will lay a foundation for the development of protease-producing bacterial agents for wastewater purification and the construction of an environment-friendly tropical aquaculture model.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Community Structure of Protease-Producing Bacteria Cultivated From Aquaculture Systems: Potential Impact of a Tropical Environment

Wei

Long

et al. 2021

Front. Microbiol.

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“…The proteolytic bacteria if included in aquaculture may serve multiple purposes such as (1) improved digestion of proteinrich substances present in the host's natural diet and in commercial feed resulting in an increased growth of the host [15]; (2) enhancement of nonspecific immune response in the host [16]; (3) reduction of organic pollutants produced in aquaculture from the undigested feed [17] etc. Besides, as compared to exogenous proteases, use of the protease producing microbes are more ecofriendly and easy in the application in aquaculture [18].…”

Section: Introductionmentioning

confidence: 99%

“…To address the increasing demand of protease producing bacteria in industry, research and aquaculture, we focused on obtaining proteolytic strains from the gastrointestinal tract (GIT) of Nile tilapia. Fish GIT has been recognized as an excellent source of bacteria producing extracellular hydrolytic enzymes [15], and there is also a general consensus that the bacteria to be included in the animal feed should be isolated from GIT of the animals where they will be applied [18]. Consequently, we've isolated cultivable GI bacteria from Nile tilapia and screened them for protease production.…”

Section: Introductionmentioning

confidence: 99%

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Hossain

Das

Ali

et al. 2021

Preprint

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Vertebrate intestine appears an excellent source of proteolytic bacteria for industrial and probiotic use. We therefore aimed obtaining the gut-associated proteolytic species of Nile tilapia. We have isolated twenty six bacterial strains from its intestinal tract, seven of which showed exoprotease activity with the formation of clear halos on skim milk. Their depolymerization ability was further assessed on three distinct proteins including casein, gelatin and albumin. All the isolates could successfully hydrolyze the three substrates indicating relatively broad specificity of their secreted proteases. Molecular taxonomy and phylogeny of the proteolytic isolates were determined based on their 16S rRNA gene barcoding which suggested that the seven strains belong to three phyla viz. Firmicutes, Proteobacteria and Actinobacteria, distributed across the genera Priestia, Citrobacter, Pseudomonas, Stenotrophomonas, Burkholderia, Providencia and Micrococcus. The isolates were further characterized by a comprehensive study of their morphological, cultural, cellular and biochemical properties which were consistent with the phylogenetic annotations. To reveal their proteolytic capacity alongside substrate preferences, enzyme-production was determined by the diffusion assay. The Pseudomonas, Stenotrophomonas and Micrococcus isolates appeared most promising with maximum protease production on casein, gelatin and albumin media respectively. Our findings present valuable insights into the phylogenetic and biochemical properties of gut-associated proteolytic strains of Nile tilapia.

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“…The fermentation would take over one month normally to form flavor and nutrition (Zhu et al., 2019). Proteases could hydrolysis protein effectively by breaking down the peptide bond that links amino acid to poly‐peptide chain, and they account for over half of the total world enzyme markets in the world (Amin, 2018; Moradi, Sun, Song, & Hu, 2019; Raval, Pillai, Rawal, & Singh, 2014; Uttatree & Charoenpanich, 2018). Protease from microorganisms have been studied due to their excellent characteristic and highly yield, and bacterial proteases have been exploited the most widely compared with fungi, plants, and animal (Olajuyigbe & Falade, 2014; Rekik et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Virgibacillus halodenitrificans ST‐1 for fermentation of shrimp paste and hydrolysates of its protease

Liu¹,

Feng²,

Lai³

et al. 2020

Food Science & Nutrition

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Shrimp paste is one of the traditional foods in eastern China, even in East Asia (Phewpan et al., 2020). The fresh shrimps are fermented naturally by adding high concentration of salt. The fermentation would take over one month normally to form flavor and nutrition (Zhu et al., 2019). Proteases could hydrolysis protein effectively by breaking down the peptide bond that links amino acid to poly-peptide chain, and they account for over half of the total world enzyme markets in the world (

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Marine protease-producing bacterium and its potential use as an abalone probiont

Cited by 28 publications

References 26 publications

Community Structure of Protease-Producing Bacteria Cultivated From Aquaculture Systems: Potential Impact of a Tropical Environment

Community Structure of Protease-Producing Bacteria Cultivated From Aquaculture Systems: Potential Impact of a Tropical Environment

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Virgibacillus halodenitrificans ST‐1 for fermentation of shrimp paste and hydrolysates of its protease

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