Marinilactibacillus piezotolerans sp. nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough

Toffin, Laurent; Zink, Klaus-Gerhard; Kato, Chiaki; Pignet, Patricia; Bidault, Adeline; Bienvenu, Nadège; Birrien, Jean‐Louis; Prieur, Daniël

doi:10.1099/ijs.0.63236-0

Cited by 66 publications

(37 citation statements)

References 36 publications

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“…In contrast, very few studies have examined the stress response of LAB to alkaline environments. Several alkali-tolerant/alkaliphilic LAB, which are capable of growing at pH values of Ͼ9.5 as well as producing large amounts of lactic acid from glucose, have been reported to date: Enterococcus hirae (formerly Streptococcus faecalis) (8), Pediococcus urinaeequi (23), Alkalibacterium olivapovliticus (12,24), Marinilactibacillus psychrotolerans (12), M. piezotolerans (27), Halolactibacillus halophilus, and H. miurensis (13).…”

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confidence: 99%

Diversity and Mechanisms of Alkali Tolerance in Lactobacilli

Sawatari

Yokota

2007

Appl Environ Microbiol

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We determined the maximum pH that allows growth (pHmax) for 34 strains of lactobacilli. High alkali tolerance was exhibited by strains of Lactobacillus casei, L. paracasei subsp. tolerans, L. paracasei subsp. paracasei, L. curvatus, L. pentosus, and L. plantarum that originated from plant material, with pHmax values between 8.5 and 8.9. Among these, L. casei NRIC 1917 and L. paracasei subsp. tolerans NRIC 1940 showed the highest pHmax, at 8.9. Digestive tract isolates of L. gasseri, L. johnsonii, L. reuteri, L. salivarius subsp. salicinius, and L. salivarius subsp. salivarius exhibited moderate alkali tolerance, with pHmax values between 8.1 and 8.5. Dairy isolates of L. delbrueckii subsp. bulgaricus, L. delbrueckii subsp. lactis, and L. helveticus exhibited no alkali tolerance, with pHmax values between 6.7 and 7.1. Measurement of the internal pH of representative strains revealed the formation of transmembrane proton gradients (⌬pH) in a reversed direction (i.e., acidic interior) at alkaline external-pH ranges, regardless of their degrees of alkali tolerance. Thus, the reversed ⌬pH did not determine alkali tolerance diversity. However, the ⌬pH contributed to alkali tolerance, as the pHmax values of several strains decreased with the addition of nigericin, which dissipates ⌬pH. Although neutral external-pH values resulted in the highest glycolysis activity in the presence of nigericin regardless of alkali tolerance, substantial glucose utilization was still detected in the alkali-tolerant strains, even in a pH range of between 8.0 and 8.5, at which the remaining strains lost most activity. Therefore, the alkali tolerance of glycolysis reactions contributes greatly to the determination of alkali tolerance diversity.Lactic acid bacteria (LAB) produce lactic acid as a main product of sugar fermentation. Many studies have examined the response of LAB to acid stress, including the mechanisms underlying acid tolerance (3,4,28). In contrast, very few studies have examined the stress response of LAB to alkaline environments. Several alkali-tolerant/alkaliphilic LAB, which are capable of growing at pH values of Ͼ9.5 as well as producing large amounts of lactic acid from glucose, have been reported to date: Enterococcus hirae (formerly Streptococcus faecalis) (8), Pediococcus urinaeequi (23), Alkalibacterium olivapovliticus (12, 24), Marinilactibacillus psychrotolerans (12), M. piezotolerans (27), Halolactibacillus halophilus, and H. miurensis (13).The mechanism underlying the alkali tolerance of LAB has been examined in E. hirae ATCC 9790T . Biochemical experiments and mutant analyses suggest that E. hirae ATCC 9790 T maintains an internal pH lower than the external pH by the operation of an ATP-driven K ϩ /H ϩ antiporter during growth at an external pH of around 9.5 (14-17). However, E. hirae ATCC 9790T has no such system to maintain a near-neutral internal pH in an alkaline medium (22). Thus, the contribution of internal-pH maintenance to alkali tolerance in this bacterium is controversial. Adaptation to alkaline stre...

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Diversity and Mechanisms of Alkali Tolerance in Lactobacilli

Sawatari

Yokota

2007

Appl Environ Microbiol

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“…The pressure range for growth of AT7 and AT12 was 0.1 to 60 MPa, with an optimum at 15 MPa (58). Interestingly the sister taxon Marinilactibacillus contains only two marine isolates, one psychrophilic (28) and the other psychropiezotolerant (51).…”

Section: Resultsmentioning

confidence: 99%

The Unique 16S rRNA Genes of Piezophiles Reflect both Phylogeny and Adaptation

Lauro

Chastain

Blankenship

et al. 2007

Appl Environ Microbiol

124

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In the ocean's most extreme depths, pressures of 70 to 110 megapascals prevent the growth of all but the most hyperpiezophilic (pressure-loving) organisms. The physiological adaptations required for growth under these conditions are considered to be substantial. Efforts to determine specific adaptations permitting growth at extreme pressures have thus far focused on relatively few ␥-proteobacteria, in part due to the technical difficulties of obtaining piezophilic bacteria in pure culture. Here, we present the molecular phylogenies of several new piezophiles of widely differing geographic origins. Included are results from an analysis of the first deep-trench bacterial isolates recovered from the southern hemisphere (9.9-km depth) and of the first grampositive piezophilic strains. These new data allowed both phylogenetic and structural 16S rRNA comparisons among deep-ocean trench piezophiles and closely related strains not adapted to high pressure. Our results suggest that (i) the Circumpolar Deep Water acts as repository for hyperpiezophiles and drives their dissemination to deep trenches in the Pacific Ocean and (ii) the occurrence of elongated helices in the 16S rRNA genes increases with the extent of adaptation to growth at elevated pressure. These helix changes are believed to improve ribosome function under deep-sea conditions. Low temperature and high hydrostatic pressure structure deep-sea communities outside of hydrothermal vents. Tight selection by these and other environmental parameters is considered the cause of the conspicuous absence of many deep-sea taxonomic groups from the deepest ocean environments (8, 44).Both temperature and pressure exert their effects at many levels of bacterial physiology, from the structure of macromolecules to the rate of metabolic reactions. Adaptations to low temperature include alterations of membrane phospholipids, such as increased fatty acid unsaturation (43), enzymes characterized by high catalytic efficiency and reduced activation enthalpy (16,20,37,45), and high levels of cold shock proteins, RNA helicases (9), and posttranscriptional modification of tRNA molecules (15), all of which may reduce the formation of unfavorable nucleic acid secondary structures at low temperature. In contrast with enthalpy-based temperature effects, the underlying cause of pressure effects arises from the promotion of reduced system volumes, in accordance with Le Chatelier's principle (5). Despite these thermodynamic differences, low temperature and high pressure share a surprising number of influences on biological processes. For example, membrane fluidity, permeability, and phase are similarly altered by both parameters.As with psychrophiles, piezophiles ("high-pressure-loving" microbes) contain lipids with highly unsaturated fatty acids (6, 7). Indeed, the presence of unsaturated fatty acids is critical to growth ability at high pressure (3,4,19). Both low temperature and high pressure also alter protein quaternary structure (46) and nucleic acid secondary structure (50), and at ...

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“…Recently, members of the genus Marinilactibacillus have been isolated: Marinilactibacillus sp. from coastal sub-seafloor sediment of the Okhotsk Sea (Inagaki et al, 2003) and Marinilactibacillus piezotolerans from deep sub-seafloor sediment of the Nankai Trough (Toffin et al, 2005). Marinilactibacillus psychrotolerans, which has been isolated from a decaying marine alga, a living sponge and a fresh shellfish, is a marine-inhabiting lactic acid bacterium with growth optima at 2?0-3?75 % NaCl and pH 8?5-9?0 (Ishikawa et al, 2003b).…”

Section: Carbon Compoundmentioning

confidence: 99%

Halolactibacillus halophilus gen. nov., sp. nov. and Halolactibacillus miurensis sp. nov., halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in Bacillus rRNA group 1

Ishikawa

Nakajima

Itamiya

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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Eleven novel strains of marine-inhabiting lactic acid bacteria that were isolated from living and decaying marine organisms collected from a temperate area of Japan are described. The isolates were motile with peritrichous flagella and non-sporulating. They lacked catalase, quinones and cytochromes. Fermentation products from glucose were lactate, formate, acetate and ethanol. Lactate yield as percentage conversion from glucose was affected by the pH of the fermentation medium: ∼55 % at the optimal growth pH of 8·0, greater than ∼70 % at pH 7·0 and less than ∼30 % at pH 9·0. The molar ratio of the other three products was the same at each cultivation pH, approximately 2 : 1 : 1. Carbohydrates and related compounds were aerobically metabolized to acetate and pyruvate as well as lactate. The isolates were slightly halophilic, highly halotolerant and alkaliphilic. The optimum NaCl concentration for growth was 2·0–3·0 % (w/v), with a range of 0–25·5 %. The optimum pH for growth was 8·0–9·5, with a range of 6·0–10·0. The G+C content of the DNA was 38·5–40·7 mol%. The isolates constituted two genomic species (DNA–DNA relatedness of less than 41 %) each characterized by sugar fermentation profiles. The cell-wall peptidoglycan of both phenotypes contained meso-diaminopimelic acid. The major cellular fatty acids were C16 : 0 and a-C13 : 0. Comparative sequence analysis of the 16S rRNA genes revealed that these isolates represent novel species constituting a phylogenetic unit outside the radiation of typical lactic acid bacteria and an independent line of descent within the group composed of the halophilic/halotolerant/alkaliphilic and/or alkalitolerant species in Bacillus rRNA group 1, with 94·8–95·1 % similarity to the genus Paraliobacillus, 93·7–94·1 % to the genus Gracilibacillus and 93·8–94·2 % to Virgibacillus marismortui. On the basis of possession of physiological and biochemical characteristics common to typical lactic acid bacteria within Bacillus rRNA group 1, chemotaxonomic characteristics and phylogenetic independence, a new genus and two species, Halolactibacillus halophilus gen. nov., sp. nov. and Halolatibacillus miurensis sp. nov., are proposed. The type strains are Halolactibacillus halophilus M2-2T (=DSM 17073T=IAM 15242T=NBRC 100868T=NRIC 0628T) (G+C content 40·2 mol%) and Halolactibacillus miurensis M23-1T (=DSM 17074T=IAM 15247T=NBRC 100873T=NRIC 0633T) (G+C content 38·5 mol%).

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Marinilactibacillus piezotolerans sp. nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough

Cited by 66 publications

References 36 publications

Diversity and Mechanisms of Alkali Tolerance in Lactobacilli

Diversity and Mechanisms of Alkali Tolerance in Lactobacilli

The Unique 16S rRNA Genes of Piezophiles Reflect both Phylogeny and Adaptation

Halolactibacillus halophilus gen. nov., sp. nov. and Halolactibacillus miurensis sp. nov., halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in Bacillus rRNA group 1

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