Marinomonas spartinae sp. nov., a novel species with plant-beneficial properties

Lucena, Teresa; Mesa, Jennifer; Rodríguez‐Llorente, Ignacio D.; Pajuelo, Eloísa; Caviedes, Miguel A.; Ruvira, María A.; Pujalte, María J.

doi:10.1099/ijsem.0.000929

Cited by 20 publications

(8 citation statements)

References 19 publications

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“…Interestingly, all five M1 residues are conserved in both psychrophilic ( Marinomonas sp. ef1, M. artica and Paragliecola artica ) and psychrotolerant bacteria ( M. spartinae and M. shanghaiensis ) [46,47]. Although these data are based on a small set of uncharacterized enzymes, results support the hypothesis that Mn 2+ coordination is a mesophilic trait acquired or maintained by cold‐active enzymes to adapt to mild temperatures.…”

Section: Discussionmentioning

confidence: 75%

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A cold‐active esterase enhances mesophilic properties through Mn²⁺ binding

et al. 2022

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A key aspect of adaptation to cold environments is the production of cold‐active enzymes by psychrophilic organisms. These enzymes not only have high activity at low temperatures, but also exhibit remarkable structural flexibility and thermolability. In this context, the role of metal ions has been little explored, and the few available studies seem to suggest that metal binding counteracts structural flexibility. This article reports an investigation into the role of the binding of manganese ion (Mn2+) in the thermal adaptation of an esterase (M‐Est) of the GDSx family, identified in the genome of the Antarctic bacterium Marinomonas sp. ef1. M‐Est is specific for esters containing acetate groups and turned out to be a highly thermolabile cold‐active enzyme, with a catalysis optimum temperature of 5 °C and a melting temperature of 31.7 °C. A combination of biochemical and computational analyses, including molecular dynamics simulations, revealed that M‐Est binds Mn2+ ions via a single binding site located on the surface of the enzyme, close to the active site. Although the interaction between M‐Est and Mn2+ induces only local conformational changes involving the active site, quite surprisingly they trigger an improvement in both thermal stability and catalytic efficiency under mild temperature conditions. These results, together with the conservation of the Mn2+ binding site among psychrophilic and psychrotolerant homologues, suggest that Mn2+ binding may be a useful, albeit atypical, strategy to mitigate the detrimental effects of temperature on true cold‐active enzymes.

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Section: Discussionmentioning

confidence: 75%

“…Enzymes from Marinomonas sp. are a promising starting point to study temperature adaptation due to the wide distribution of these bacteria in various marine environments [44,[46][47][48][49][50]. M-Est is a cold-active enzyme, with a T opt of 5 °C and marked thermolability.…”

Section: Introductionmentioning

confidence: 99%

A cold‐active esterase enhances mesophilic properties through Mn²⁺ binding

et al. 2022

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“…sunshinyii is an endophyte and might form a specific association with marine flat grasses. Endophytic colonizers of salt marsh halophytes are also known from Saccharospirillum and Marinomonas , neighbor genera of Gynuelld 65 , 66 within the order Oceanospirillales. Thus, the rhizosphere of marine plants might be an attractive discovery resource harboring bacteria with an ecology and chemistry distinct from classical producer groups.…”

Section: Discussionmentioning

confidence: 99%

Genome-based discovery and total synthesis of janustatins, potent cytotoxins from a plant-associated bacterium

et al. 2022

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Host-associated bacteria are increasingly recognized as underexplored sources of bioactive natural products with unprecedented chemical scaffolds. A recently identified example is the plant root-associated marine bacterium Gynuella sunshinyii of the chemically underexplored order Oceanospirillales. Its genome contains at least 22 biosynthetic gene clusters suggesting a rich and mostly uncharacterized specialized metabolism. In this work, in silico chemical prediction of a non-canonical polyketide synthase cluster led to the discovery of janustatins, structurally unprecedented polyketide alkaloids with potent cytotoxicity that are produced at minute quantities. A combination of MS and 2D NMR experiments, density functional theory calculations of 13 C chemical shifts, and semiquantitative interpretation of T-ROESY data were conducted to determine the relative configuration, which enabled the total synthesis of both enantiomers and assignment of the absolute configuration. Janustatins feature a previously unknown pyridodihydropyranone heterocycle and an unusual biological activity consisting of delayed, synchronized cell death at subnanomolar concentrations.

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“…A previous study shows that Flavobacteria respond to the blooms quickly with successional patterns over seven days to weeks (Buchan et al 2014). Members of Flavobacteria also have been reported to have algicidal activity, which may cause the deadline of the algal blooms (Lucena et al 2016). Rhodobacteraceae are major vitamin suppliers in the marine environment, about 90% of which have complete vitamin B12 synthesis pathways (Croft et al 2005).…”

Section: The Dynamics Of the Prokaryotic Communities During The Algal...mentioning

confidence: 99%

The responses of prokaryotic communities to the algal blooms from the Pearl River estuary

Lin

Liu

Lei

et al. 2022

Preprint

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Algal blooms are severe ecological disasters in the marine environment, affecting the biogeochemical cycles substantially. It is critical to explore how the prokaryotic community responds to the dynamics of algal blooms in the marine environment. The shifts in prokaryotic communities during the algal blooms have been extensively investigated, while the interactions and assembly mechanisms of prokaryotic communities are still incompletely understood. We conducted nutrient addition cultivations for the brackish water from the Pearl River estuary to simulate the algal bloom process and monitored the prokaryotic community compositions over forty days. Results showed evident differences between blooming and after-bloom stages of prokaryotic communities in diversity and taxonomic compositions. Bacillus, Gimesiaceae, and Fibrobacteraceae were dominant before the cultivation. Mesoflavibacter, Rhodobacteraceae, and Acinetobacter were accumulated in the blooming stage. Acinetobacter, Comamonadaceae, and Gimesia were enriched in the after-blooming stage, while Mesoflavibacter, Rhodobacteraceae, and Acinetobacter were active during the whole blooming period. Co-occurrence networks analysis showed that prokaryotic interactions were predominantly driven by positive relationships that impacted the algal blooming fates. Rhodobacteraceae, Flavobacteriaceae, Winogradskyella, and Pseudomonas are the keystone groups of the prokaryotic communities in the blooming stage network, while Marinobacter, Thalassobaculum, Actinobacteria, Flavobacterium, and Rhodobacteraceae are the keystone groups of the after-bloom stage network. Functional prediction by FAPROTAX showed that dissimilatory nitrate reduction increased in the after-bloom stage. Our study revealed the dynamic of the prokaryotic communities and the characteristics of their co-occurrent profiles, which shed light on revealing the potential functions of prokaryotic behaviors during estuarine algal blooming events.

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Marinomonas spartinae sp. nov., a novel species with plant-beneficial properties

Cited by 20 publications

References 19 publications

A cold‐active esterase enhances mesophilic properties through Mn²⁺ binding

A cold‐active esterase enhances mesophilic properties through Mn²⁺ binding

Genome-based discovery and total synthesis of janustatins, potent cytotoxins from a plant-associated bacterium

The responses of prokaryotic communities to the algal blooms from the Pearl River estuary

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Marinomonas spartinae sp. nov., a novel species with plant-beneficial properties

Cited by 20 publications

References 19 publications

A cold‐active esterase enhances mesophilic properties through Mn2+ binding

A cold‐active esterase enhances mesophilic properties through Mn2+ binding

Genome-based discovery and total synthesis of janustatins, potent cytotoxins from a plant-associated bacterium

The responses of prokaryotic communities to the algal blooms from the Pearl River estuary

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Product

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A cold‐active esterase enhances mesophilic properties through Mn²⁺ binding

A cold‐active esterase enhances mesophilic properties through Mn²⁺ binding