Marinomonas ushuaiensis sp. nov., isolated from coastal sea water in Ushuaia, Argentina, sub-Antarctica

Prabagaran, Solai Ramatchandirane; Korpole, Suresh; Manorama, R.; Delille, Daniel; Shivaji, Sisinthy

doi:10.1099/ijs.0.63363-0

Cited by 38 publications

(34 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Succession in chemosynthetic wood fall mats D Kalenitchenko et al Yücel et al, 2013;Kalenitchenko et al, 2015). During this first stage of mat formation, the Epsilonproteobacteria shared their habitat with seven Gammaproteobacteria OTUs related to the aerobic genera Thalassolituus and Marinomonas (Yakimov et al, 2004;Prabagaran et al, 2005). Our results, together with observations made on a sulfideoxidizing community in the Black sea (Jørgensen et al, 1991), suggest that this first, potentially sulfide-oxidizing mat community, has an important role in protecting the wood fall surrounding environment against hydrogen sulfide produced during the wood decay.…”

Section: Ecological Succession During Epixylic Mat Formationsupporting

confidence: 56%

Ecological succession leads to chemosynthesis in mats colonizing wood in sea water

Kalenitchenko¹,

Dupraz²,

Bris³

et al. 2016

The ISME Journal

View full text Add to dashboard Cite

Chemosynthetic mats involved in cycling sulfur compounds are often found in hydrothermal vents, cold seeps and whale falls. However, there are only few records of wood fall mats, even though the presence of hydrogen sulfide at the wood surface should create a perfect niche for sulfide-oxidizing bacteria. Here we report the growth of microbial mats on wood incubated under conditions that simulate the Mediterranean deep-sea temperature and darkness. We used amplicon and metagenomic sequencing combined with fluorescence in situ hybridization to test whether a microbial succession occurs during mat formation and whether the wood fall mats present chemosynthetic features. We show that the wood surface was first colonized by sulfide-oxidizing bacteria belonging to the Arcobacter genus after only 30 days of immersion. Subsequently, the number of sulfate reducers increased and the dominant Arcobacter phylotype changed. The ecological succession was reflected by a change in the metabolic potential of the community from chemolithoheterotrophs to potential chemolithoautotrophs. Our work provides clear evidence for the chemosynthetic nature of wood fall ecosystems and demonstrates the utility to develop experimental incubation in the laboratory to study deep-sea chemosynthetic mats.

show abstract

Section: Ecological Succession During Epixylic Mat Formationsupporting

confidence: 56%

Ecological succession leads to chemosynthesis in mats colonizing wood in sea water

Kalenitchenko¹,

Dupraz²,

Bris³

et al. 2016

The ISME Journal

View full text Add to dashboard Cite

show abstract

“…Table 1. Phenotypic characteristics that differentiate strain 328 T from other Marinomonas species Strain/species: 1, strain 328 T ; 2, M. pontica (Ivanova et al, 2005;Gupta et al, 2006); 3, M. polaris (Gupta et al, 2006); 4, M. dokdonensis (Yoon et al, 2005); 5, M. ushuaiensis (Prabagaran et al, 2005); 6, M. primoryensis (Romanenko et al, 2003;Gupta et al, 2006); 7, M. communis (Baumann et al, 1972). +, Positive; 2, negative; W, weak reaction; ND, not determined.…”

Section: Description Of Marinomonas Arctica Sp Novmentioning

confidence: 99%

“…So far, the genus Marinomonas comprises ten species: M. communis, M. vaga, M. mediterranea, M. primoryensis, M. pontica, M. ushuaiensis, M. aquimarina, M. dokdonensis, M. polaris and M. ostreistagni (Baumann et al, 1972; Solano & SanchezAmat, 1999; Romanenko et al, 2003;Ivanova et al, 2005;Prabagaran et al, 2005; Macián et al, 2005;Yoon et al, 2005;Gupta et al, 2006;Lau et al, 2006). During screening of cold-adapted bacteria from sea-ice samples of the Arctic Ocean, a novel psychrotolerant strain, designated strain 328 T , was isolated.…”

mentioning

confidence: 99%

Marinomonas arctica sp. nov., a psychrotolerant bacterium isolated from the Arctic

Zhang

Xin

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

A novel psychrotolerant, Gram-negative, motile bacterium, designated strain 328 T , was isolated from sea-ice samples collected off the Canadian Basin of the Arctic Ocean (786 239 140 N 1496 069 550 W). Strain 328 T was able to grow at 0-37 6C, with optimum growth at 25-27 6C. It possessed phosphatidylethanolamine and phosphatidylglycerol as major phospholipids and C 10 : 0 3-OH (31.78 %), C 18 : 1 v7c (27.50 %) and iso-C 15 : 0 2-OH and/or C 16 : 1 v7c (19.22 %) as predominant cellular fatty acids. The DNA G+C content of strain 328 T was 45.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 328 T was a member of the genus Marinomonas (92.7-96.0 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain 328 T was considered to represent a novel species of the genus Marinomonas. The name Marinomonas arctica sp. nov. is proposed, with strain 328 T (5CGMCC 1.6498 T 5JCM 14976 T ) as the type strain.The genus Marinomonas was proposed by the reclassification of two species of the genus Alteromonas (Baumann et al., 1972), namely Alteromonas vaga and Alteromonas communis, as Marinomonas vaga and Marinomonas communis, based on 16S rRNA gene sequences (Van Landschoot & De Ley, 1983). So far, the genus Marinomonas comprises ten species: M. communis, M. vaga, M. mediterranea, M. primoryensis, M. pontica, M. ushuaiensis, M. aquimarina, M. dokdonensis, M. polaris and M. ostreistagni (Baumann et al., 1972; Solano & SanchezAmat, 1999; Romanenko et al., 2003;Ivanova et al., 2005;Prabagaran et al., 2005; Macián et al., 2005;Yoon et al., 2005;Gupta et al., 2006;Lau et al., 2006). During screening of cold-adapted bacteria from sea-ice samples of the Arctic Ocean, a novel psychrotolerant strain, designated strain 328 T , was isolated. Based on a polyphasic taxonomic approach, strain 328 T was identified as a representative of a novel species of the genus Marinomonas.Strain 328 T was isolated from sea-ice samples collected from the Canadian Basin (78 u 239 140 N 149 u 069 550 W) using a MARK II ice auger during the Second Chinese National Arctic Research Expedition cruise of the USCGC icebreaker Xue Long into the Canadian Basin in August 2003. Sea-ice samples were cut carefully into 10-20 cm sections using a sterile saw and placed in sterile plastic bottle to be melted at 4 u C. Strain 328 T was obtained using previously described media and methods (Zhang et al., 2006). The novel isolate was routinely grown on marine agar (MA) for 2 days at 25 u C and maintained as a glycerol suspension (20 %, w/v) at 280 u C. M. polaris JCM 12522 T , obtained from the JCM, was routinely grown on MA or in marine 2216 broth (MB) at 22 u C and used as a reference strain.DNA was extracted from strain 328 T and purified as described by Sambrook et al. (1989). The 16S rRNA gene was amplified by PCR with two universal primers (Zhang et al., 2006). Purified PCR product was ligated to pMD 18-T (TaKaRa Bio) and cloned according to the manufacturer's instructions....

show abstract

“…Most recognized species of the genus have been isolated from seawater samples collected from different geographical locations: Marinomonas communis and M. vaga (formerly Alteromonas communis and A. vaga) from the Pacific Ocean (Baumann et al, 1972;van Landschoot & De Ley, 1983), Marinomonas pontica from the Black Sea (Ivanova et al, 2005), Marinomonas dokdonensis from the East Sea of Korea (Yoon et al, 2005) and Marinomonas mediterranea and M. aquimarina from the Mediterranean Sea (Solano et al, 1997;Solano & Sanchez-Amat, 1999;Macián et al, 2005). Some have been isolated from cold environments, such as subantarctic regions [Marinomonas polaris (Gupta et al, 2006) and M. ushuaiensis (Prabagaran et al, 2005)] or sea-ice samples [Marinomonas primoryensis (Romanenko et al, 2003)]. The only species described as being associated IP: 34.210.69.67…”

mentioning

confidence: 99%

Taxonomic study of Marinomonas strains isolated from the seagrass Posidonia oceanica, with descriptions of Marinomonas balearica sp. nov. and Marinomonas pollencensis sp. nov.

Espinosa

Marco‐Noales

Gómez

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Novel aerobic, Gram-negative bacteria with DNA G+C contents below 50 mol% were isolated from the culturable microbiota associated with the Mediterranean seagrass Posidonia oceanica. 16S rRNA gene sequence analyses revealed that they belong to the genus Marinomonas. Strain IVIA-Po-186 is a strain of the species Marinomonas mediterranea, showing 99.77 % 16S rRNA gene sequence similarity with the type strain, MMB-1T, and sharing all phenotypic characteristics studied. This is the first description of this species forming part of the microbiota of a marine plant. A second strain, designated IVIA-Po-101T, was closely related to M. mediterranea based on phylogenetic studies. However, it differed in characteristics such as melanin synthesis and tyrosinase, laccase and antimicrobial activities. In addition, strain IVIA-Po-101T was auxotrophic and unable to use acetate. IVIA-Po-101T shared 97.86 % 16S rRNA gene sequence similarity with M. mediterranea MMB-1T, but the level of DNA–DNA relatedness between the two strains was only 10.3 %. On the basis of these data, strain IVIA-Po-101T is considered to represent a novel species of the genus Marinomonas, for which the name Marinomonas balearica sp. nov. is proposed. The type strain is IVIA-Po-101T (=CECT 7378T =NCIMB 14432T). A third novel strain, IVIA-Po-185T, was phylogenetically distant from all recognized Marinomonas species. It shared the highest 16S rRNA gene sequence similarity (97.4 %) with the type strain of Marinomonas pontica, but the level of DNA–DNA relatedness between the two strains was only 14.5 %. A differential chemotaxonomic marker of this strain in the genus Marinomonas is the presence of the fatty acid C17 : 0 cyclo. Strain IVIA-Po-185T is thus considered to represent a second novel species of the genus, for which the name Marinomonas pollencensis sp. nov. is proposed. The type strain is IVIA-Po-185T (=CECT 7375T =NCIMB 14435T). An emended description of the genus Marinomonas is given based on the description of these two novel species, as well as other Marinomonas species described after the original description of the genus.

show abstract

Marinomonas ushuaiensis sp. nov., isolated from coastal sea water in Ushuaia, Argentina, sub-Antarctica

Cited by 38 publications

References 15 publications

Ecological succession leads to chemosynthesis in mats colonizing wood in sea water

Ecological succession leads to chemosynthesis in mats colonizing wood in sea water

Marinomonas arctica sp. nov., a psychrotolerant bacterium isolated from the Arctic

Taxonomic study of Marinomonas strains isolated from the seagrass Posidonia oceanica, with descriptions of Marinomonas balearica sp. nov. and Marinomonas pollencensis sp. nov.

Contact Info

Product

Resources

About