Background: Natural Procreative Technology (NaProTechnology) is a system of management of infertility and other reproductive health issues which requires the application of the observations made by a woman and record of key events throughout her menstrual cycle. Objectives: The study assessed the knowledge, awareness, and perception of NaProTechnology as well as the effect of an educational intervention among pharmacy undergraduate students at University of Nigeria, Nsukka. Methods: It was a cross-sectional, questionnaire-based study. The ethical approval was obtained from the research ethics committee of the Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka. At baseline, the knowledge, awareness and perception of the students were assessed. Followed by the administration of an educational video on NaProTechnology. Then a post intervention survey was done to assess the effect of the educational intervention. Key findings: There were 410 and 350 students in the pre- and post-intervention surveys respectively with relatively equal number of males and females. Majority were between 18 and 29 years old. Those in third year of study had the best knowledge (P=0.002) and awareness (P=0.021) and most positive perception (P=0.035) of NaProTechnology. The knowledge, awareness, and positive perception of NaProTechnology among the pharmacy students prior to intervention were poor but improved markedly post intervention (P < 0.001). Conclusion: A video intervention was effective in improving the short-term knowledge, awareness and positive perception of NaProTechnology among pharmacy students. We recommend the public health system approach of informing, educating and communicating NaProTechnology to pharmacists to achieve long-term effects. Well-designed courses as well as including NaProTechnology in the curriculum are specific ways that can be used to achieve this among pharmacy students. Keywords: NaProTechnology; educational-intervention; pharmacy-students; curriculum