Maritimibacter alexandrii sp. nov., a New Member of Rhodobacteraceae Isolated from Marine Phycosphere

Wang, Xing; Ye, Yun; Xu, Feifei; Duan, Yuhan; Xie, Pengfei; Qiao, Yingjie

doi:10.1007/s00284-021-02645-z

Cited by 5 publications

(5 citation statements)

References 32 publications

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“…The highest efficiency of the bioflocculanting rate of 95.7 ± 8.5% was achieved when 0.60 g·L −1 of EPS was applied. It exhibited a higher bioflocculanting capacity compared to the other bacterial strains, which were also recovered from the marine dinoflagellates previously reported [ 27 , 28 , 29 , 30 , 56 , 57 , 58 , 59 ]. To further infer whether the type and abundance of the monosaccharides of the polymer EPSs were related to the bioflocculanting activity, the characterizations of the monosaccharide compositions of the EPSs and their correlations with the bioflocculanting activity were then performed.…”

Section: Resultsmentioning

confidence: 64%

“…In addition, the genomic mining also revealed the presence of several typical biosynthesis genes (wzx, exo, and muc) for bacterial EPS biosynthesis in strain LZ-28. Thus, the present study indicated that strain LZ-28 could serve as a new, fresh bacterial candidate with natural potential for the production of versatile EPS bioflocculants derived from marine microalgae with potential pharmaceutical, environmental, and biotechnological implications [ 27 , 28 , 29 , 30 , 55 , 56 , 57 , 58 , 59 , 60 , 61 ].…”

Section: Resultsmentioning

confidence: 99%

“…The obtained samples were dehydrated in serial 30–100% ethanol, then mounted on copper grids, and observed using a JEM-1200 TEM instrument (EOL, Tokyo, Japan). Gram-staining test was followed by the our procedures previously described [ 56 , 57 , 58 ]. The hanging-drop technique was used for observing motility during the exponential phase of the culture growth.…”

Section: Methodsmentioning

confidence: 99%

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Taxonomic and Bioactivity Characterizations of Mameliella alba Strain LZ-28 Isolated from Highly Toxic Marine Dinoflagellate Alexandrium catenella LZT09

et al. 2022

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Microalgae host varied microbial consortium harboring cross-kingdom interactions with fundamental ecological significance in aquatic ecosystems. Revealing the complex biofunctions of the cultivable bacteria of phycosphere microbiota is one vital basis for deeply understanding the mechanisms governing these dynamic associations. In this study, a new light-yellow pigmented bacterial strain LZ-28 was isolated from the highly-toxic and harmful algal bloom-forming dinoflagellate Alexandrium catenella LZT09. Collective phenotypic and genotypic profiles were obtained to confidently identify this strain as a new Mameliellaalba member. Comparative genomic analysis showed that strain LZ-28 shared highly similar functional features with other four marine algae-derived M. alba strains in spite of their distinctive isolation sources. Based on the bioactivity assaying, the mutual growth-promoting effects between bacterial strain LZ-28 and algal strain LZT09 were observed. After the culture conditions were optimized, strain LZ-28 demonstrated an extraordinary production ability for its bioflocculanting exopolysaccharides (EPS). Moreover, the portions of two monosaccharides glucose and fucose of the EPS were found to positively contribute to the bioflocculanting capacity. Therefore, the present study sheds light on the similar genomic features among the selected M. alba strains, and it also reveals the potential pharmaceutical, environmental and biotechnological implications of active EPS produced by this new Mameliella alba strain LZ-28 recovered from toxic bloom-forming marine dinoflagellate.

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Section: Resultsmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Taxonomic and Bioactivity Characterizations of Mameliella alba Strain LZ-28 Isolated from Highly Toxic Marine Dinoflagellate Alexandrium catenella LZT09

et al. 2022

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“…But, this observation was not consistent in other coastal settings, where other phytoplankton groups dominated (Chamberlain et al., 2014). Synergistic interactions between Synechococcus and alphaproteobacterial heterotrophs ( Rhodobacteraceae ), gammaproteobacteria ( Alteromonadaceae ) (Dang & Lovell, 2016; Wang et al., 2021; Zheng et al., 2018), and the phylum Bacteroidetes are fundamental in the marine food web (Bae et al., 2022; Buchan et al., 2014). Given the delicate autotrophic‐heterotrophic metabolic balance in coastal marine ecosystems, SGD is an important factor that should be accounted for with respect to its chemical and biological effects on the ocean.…”

Section: Introductionmentioning

confidence: 99%

Allochthonous Groundwater Microorganisms Affect Coastal Seawater Microbial Abundance, Activity and Diversity

Yanuka‐Golub,

Belkin,

Weber

et al. 2024

JGR Biogeosciences

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Submarine groundwater discharge (SGD) is a globally important process supplying nutrients and trace elements to the coastal environment, thus playing a pivotal role in sustaining marine primary productivity. Along with nutrients, groundwater also contains allochthonous microbes that are discharged from the terrestrial subsurface into the sea. Currently, little is known about the interactions between groundwater‐borne and coastal seawater microbial populations, and groundwater microbes' role upon introduction to coastal seawater populations. Here, we investigated seawater microbial abundance, activity and diversity in a site strongly influenced by SGD. In addition, through laboratory‐controlled bottle incubations, we mimicked different mixing scenarios between groundwater and seawater. Our results demonstrate that the addition of 0.1 μm filtered groundwater stimulated heterotrophic activity and increased microbial abundance compared to control coastal seawater, whereas 0.22 μm filtration treatments induced primary productivity and Synechococcus growth. 16S rRNA gene sequencing showed a strong shift from a SAR11‐rich community in the control samples to Rhodobacteraceae dominance in the <0.1 μm treatment, in agreement with Rhodobacteraceae enrichment in the SGD field site. These results suggest that microbes delivered by SGD may affect the abundance, activity and diversity of intrinsic microbes in coastal seawater, highlighting the cryptic interplay between groundwater and seawater microbes in coastal environments, which has important implications for carbon cycling.

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“…Marine Synechococcus are ubiquitous cyanobacteria in the global ocean and is abundant in both estuarine and coastal waters (Dufresne et al, 2008;Huang et al, 2012). Synergistic interactions between Synechococcus and alphaproteobacteria heterotrophic bacteria (Rhodobacteraceae), gammaproteobacteria (Alteromonadaceae) (Dang and Lovell, 2016;Wang et al, 2021;Zheng et al, 2018), and the Cytophaga-Flavobacteria-Bacteroides (CFB) group are fundamental in the marine food web (Buchan et al, 2014). In oligotrophic surface oceans, the three bacterial groups Prochlorococcus, marine Synechococcus, and heterotrophs in the SAR11 clade often represent more than 50% of free-living bacterial cells (Becker et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Allochthonous groundwater microorganisms affect coastal seawater microbial abundance, activity and diversity

Yanuka-Golub,

Belkin,

Weber

et al. 2023

Preprint

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Submarine groundwater discharge (SGD) is a globally important process supplying nutrients and trace elements to the coastal environment, thus playing a pivotal role in sustaining marine primary productivity. Along with nutrients, groundwater also contains allochthonous microbes that are discharged from the terrestrial subsurface into the sea. Currently, little is known about the interactions between groundwater-borne and coastal seawater microbial populations, and their role upon introduction to coastal seawater populations. Here, we investigated seawater microbial abundance, activity and diversity in a site strongly influenced by SGD (in-situ observations), and through laboratory-controlled bottle incubations mimicking different mixing scenarios between SGD (either ambient or filtered through 0.1 um/0.22 um) and seawater. Our results demonstrate that the addition of <0.1 um SGD stimulated heterotrophic activity and increased microbial abundance compared to control, whereas <0.22 um filtration treatments induced primary productivity rates and Synechococcus growth. Amplicon sequencing of the 16S rRNA gene showed a strong shift from a SAR11-rich community in the reference SGD-unaffected coastal samples to a Rhodobacteraceae-dominated one in the <0.1 um treatment, in agreement with their in-situ enrichment in the SGD field site. These results suggest that despite the significant nutrient input, microbes delivered by SGD may affect the abundance, activity and diversity of intrinsic microbes in coastal seawater. Our results highlight the cryptic interplay between groundwater and seawater microbes in coastal environments, which has important implications for carbon cycling and climate.

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Maritimibacter alexandrii sp. nov., a New Member of Rhodobacteraceae Isolated from Marine Phycosphere

Cited by 5 publications

References 32 publications

Taxonomic and Bioactivity Characterizations of Mameliella alba Strain LZ-28 Isolated from Highly Toxic Marine Dinoflagellate Alexandrium catenella LZT09

Taxonomic and Bioactivity Characterizations of Mameliella alba Strain LZ-28 Isolated from Highly Toxic Marine Dinoflagellate Alexandrium catenella LZT09

Allochthonous Groundwater Microorganisms Affect Coastal Seawater Microbial Abundance, Activity and Diversity

Allochthonous groundwater microorganisms affect coastal seawater microbial abundance, activity and diversity

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