Maritimibacter harenae sp. nov. and Sneathiella litorea sp. nov.: members of Alphaproteobacteria isolated from sea sand

Khan, Shehzad Abid; Jung, Hye Su; Park, Hye Yoon; Jeon, Che Ok

doi:10.1007/s10482-021-01559-x

Cited by 7 publications

(13 citation statements)

References 27 publications

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“…Small amounts of aliquots (100 µl) of each serial dilution were spread on marine agar 2216 (MA; BD, USA) and the agar plates were incubated aerobically for 7 days at 25°C. The 16S rRNA genes of colonies grown on MA were ampli ed by PCR using the universal primers F1 (5′-AGA GTT TGA TCM TGG CTC AG-3′) and R13 (5′-TAC GGY TAC CTT GTT ACG ACT T-3′) (Khan et al 2021). The PCR amplicons were double-digested with HaeIII and HhaI and analysed on 2% agarose electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

“…The PCR amplicons were double-digested with HaeIII and HhaI and analysed on 2% agarose electrophoresis. Representative PCR amplicons showing unique fragment patterns were partially sequenced using the universal primer 340F (5′-CCT ACG GGA GGC AGC AG-3′) (Khan et al 2021) and the sequences were compared with those of all type strains of validly published species in the EZBioCloud server (https://www.ezbiocloud.net/identify) (Yoon et al 2017). Two putative novel strains belonging to the genus Hoe ea, designated as G2-23 T and J2-29 T , were selected for further taxonomic characterisations.…”

Section: Methodsmentioning

confidence: 99%

“…The PCR amplicons of strains G2-23 T and J2-29 T ampli ed by the primers F1 and R13 were further sequenced using the universal primers 518R (5 -ATT ACC GCG GCT GCT GG-3 ) and 805F (5 -GAT TAG ATA CCC TGG TAG TC-3 ) (Khan et al 2021). The 16S rRNA gene sequences obtained by 340F, 518R, and 805F primers were assembled, resulting in obtaining almost-complete 16S rRNA gene sequences of strains G2-23 T (1,429 nucleotides) and J2-29 T (1,425 nucleotides).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

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Hoeflea algicola sp. nov. and Hoeflea ulvae sp. nov., isolated from phycosphere of marine algae

Kristyanto

Kim

Han

et al. 2023

Preprint

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Two Gram-negative, moderately halophilic, and motile rod bacteria, strains G2-23T and J2-29T, showing catalase- and oxidase-positive activities were isolated from marine algae. Both strains optimally grew at 30°C, pH 7.0, and 2% (w/v) NaCl. Both strains contained ubiquinone-10 as the sole isoprenoid quinone. As major cellular fatty acids and polar lipids, strain G2-23T contained summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, and summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c/ω6c) and phosphatidylethanolamine (PE), phosphatidyl-N-monomethylethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol, sulfoquinovosyl diacylglycerol (SQDG), and an unidentified phospholipid (PL), respectively, while strain J2-29T contained summed feature 8, C18:1 ω7c 11-methyl, and C16:0 and PE, PME, PG, SQDG, and PL, respectively. The genomic DNA G + C contents of strains G2-23T and J2-29T were 59.5 and 62.2 mol%, respectively. Both strains shared 97.9% 16S rRNA gene sequence similarity, 79.8% average nucleotide identity (ANI), and 22.8% digital DNA-DNA hybridisation (DDH) value, indicating that they are different species. Phylogenetic and phylogenomic analyses by 16S rRNA gene and genome sequences, respectively, revealed that strains G2-23T and J2-29T formed different phylogenic lineages within the genus Hoeflea. ANI and digital DDH values between strains G2-23T and J2-29T and other Hoeflea type strains were less than 79.0 and 22.1% and 80.5 and 23.3%, respectively, representing that they are novel species of the genus Hoeflea. Based on the phenotypic, chemotaxonomic, and molecular properties, strains G2-23T and J2-29T represent two different novel species of the genus Hoeflea, for which the names Hoeflea algicola sp. nov. (G2-23T = KACC 22714T = JCM 35548T) and Hoeflea ulvae sp. nov. (J2-29T = KACC 22715T = JCM 35549T), respectively, are proposed.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Phylogenetic Analysismentioning

confidence: 99%

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Hoeflea algicola sp. nov. and Hoeflea ulvae sp. nov., isolated from phycosphere of marine algae

Kristyanto

Kim

Han

et al. 2023

Preprint

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“…Strains H242 T and B156 T were isolated from flatland near a river (37°15′04.2′′N, 128°32′26.3′′E) and mountain (37°15′38.7′′N, 128°36′28.6′′E), respectively, in Gangwon Province of South Korea, according to a previously described procedure [ 10 ]. In brief, soil samples were collected in sterile plastic tubes and stored in an icebox for transport.…”

Section: Methodsmentioning

confidence: 99%

Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., Isolated from Soil

Khan

Kim

Baek

et al. 2021

J. Microbiol. Biotechnol.

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The genus Ramlibacter, a new genus of the family Comamonadaceae in the class Betaproteobacteria, was first proposed by Heulin et al. with Ramlibacter tataouinensis as the type species, which was isolated from subdesert soil [1]. At the time of writing, the genus comprises 10 validly published species (https://lpsn.dsmz.de/genus/ ramlibacter) isolated primarily from soil habitats, such as subdesert soil [1], ginseng soil [2, 3], forest soil [4-6], and garden soil [7,8]. An exception is Ramlibacter aquaticus LMG 30558 T , which was isolated from a water environment [9]. The genus Ramlibacter includes gram-negative, catalase-positive, oxidase-variable, aerobic, and non-motile (by flagella) rod or coccoid bacteria containing ubiquinone-8 (Q-8) and C 16:0 , cyclo-C 17:0 , and summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c) as the major respiratory quinone and fatty acids, respectively [1][2][3][4][5][6][7][8][9]. The DNA G+C content of Ramlibacter species ranges from 62.0-70.6 mol%. In this study, we isolated two strains, presumably representing novel species of the genus Ramlibacter, from soil samples collected from different areas in South Korea and taxonomically characterized them using a polyphasic approach. Material and Methods Isolation and CultivationStrains H242 T and B156 T were isolated from flatland near a river (37°15′04.2′′N, 128°32′26.3′′E) and mountain (37°15′38.7′′N, 128°36′28.6′′E), respectively, in Gangwon Province of South Korea, according to a previously described procedure [10]. In brief, soil samples were collected in sterile plastic tubes and stored in an icebox for transport. The soil samples were resuspended and serially diluted in phosphate-buffered saline (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , pH 7.2). The serially diluted aliquots (100 μl) were spread on R2A agar (BD, USA), and the agar plates were incubated aerobically at 30 °C for 3 d. The 16S rRNA genes of colonies grown on R2A agar were PCR-amplified using the universal primers F1 (5′-AGA GTT TGA TCM TGG CTC AG-3′) and R13 (5′-TAC GGY TAC CTT GTT ACG ACT T-3′) and double-digested with HaeIII and HhaI. The representative PCR amplicons of 16S rRNA genes showing different fragment patterns were partially sequenced using the universal primer 340F (5′-CCT ACG GGA GGC AGC AG-3′) at Macrogen (Korea). The resulting sequences were compared with all validly reported type strains using the Nucleotide Similarity Search program on Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242 T and B156 T , were isolated from soil in South Korea. Cells of strain H242 T were oxidase-positive and non-motile short rods, while those of strain B156 T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C 16:0 , cyclo-C 17:0 , and summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids an...

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“… Sneathiella aquimaris [5] was isolated from a Penaeus vannamei aquaculture seawater sample in 2018. ‘ Sneathiella litorea ’ [6] was isolated from sea sand in the Republic of Korea in 2021 and Sneathiella sedimenti [7] was isolated from coastal sediment sample in 2022.…”

Section: Introductionmentioning

confidence: 99%

Sneathiella marina sp. nov., isolated from a sea anemone in the Western Pacific Ocean

Wei

Lai

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel marine bacterium designated strain PHK-P5T was isolated from a sea anemone (Actinostolidae sp. 1). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PHK-P5T belonged to the genus Sneathiella . The bacterium was Gram-stain-negative, aerobic, oxidase- and catalase- positive, oval- to rod-shaped, and motile. Growth was observed at pH 6.0–9.0, salinities of 2.0–9.0 % and temperatures of 4–37 °C. The G+C content of the chromosomal DNA was 49.2 %. The respiratory quinone was determined to be Q-10. The principal fatty acids of strain PHK-P5T were C19 : 0cyclo ω8c (25.19 %), C16 : 0 (22.76 %), summed feature 8 (C18 : 1 ω7c/ω6c; 16.14 %), C14 : 0 (8.81 %), C17 : 0cyclo (8.10 %), summed feature 2 (C12 : 0 aldehyde and/or unknown 10.928; 7.19 %) and C18 : 1 ω7c 11-methyl (5.03 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The average nucleotide identity and digital DNA–DNA hybridization values among the genomes of strain PHK-P5T and the reference strains were 68.7–70.9 % and 17.4–18.1 %, respectively. The combined genotypic and phenotypic data showed that strain PHK-P5T represents a novel species within the genus Sneathiella , for which the name Sneathiella marina sp. nov. is proposed, with the type strain PHK-P5T (=MCCC M21824T=KCTC 82924T).

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Maritimibacter harenae sp. nov. and Sneathiella litorea sp. nov.: members of Alphaproteobacteria isolated from sea sand

Cited by 7 publications

References 27 publications

Hoeflea algicola sp. nov. and Hoeflea ulvae sp. nov., isolated from phycosphere of marine algae

Hoeflea algicola sp. nov. and Hoeflea ulvae sp. nov., isolated from phycosphere of marine algae

Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., Isolated from Soil

Sneathiella marina sp. nov., isolated from a sea anemone in the Western Pacific Ocean

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