We examined the effect of running velocity upon magnitude and range of asymmetry in the main kinetics and kinematics of treadmill running at constant, submaximal velocities. Nine well-trained, uninjured distance runners ran, in a random order, at seven running velocities (10, 12.5, 15, 17.5, 20, 22.5, and 25 km.h −1) for 60 s (separated by > 90 s of rest) on an instrumented treadmill (ADAL3D-WR, Medical Development, France). Continuous measurement (1,000 Hz) of spatio-temporal, horizontal force production, and spring-mass characteristics was performed and data over 10 consecutive steps (5 right and 5 leg foot contacts after ∼50 s of running) were used for subsequent comparisons. Group mean and the range of asymmetry scores were assessed from the "symmetry angle" (SA) formulae where a score of 0%/100% indicates perfect symmetry/asymmetry. Mean SA scores for spatio-temporal variables were lower than 2%: contact time (0.6 ± 0.1%; range: 0.4-0.7%), aerial time (1.7 ± 0.2%; range: 1.3-2.1%) as well as step length and step frequency (0.7 ± 0.2%; range: 0.5-0.9%). Mean loading rate (5.3 ± 1.1%; range: 4.1-6.9%) and spring mass model [peak vertical force: 3.2 ± 1.6% (range: 2.9-3.4%); maximal downward vertical displacement: 11.2 ± 6.0% (range: 9.2-14.0%); leg compression: 3.6 ± 1.9% (range: 2.9-5.6%); vertical stiffness: 8.8 ± 1.9% (range: 7.1-11.6%); leg stiffness: 1.6 ± 0.6% (range: 1.2-2.9%)] presented larger mean SA values. Mean SA scores ranged 1-4% for duration of braking (1.3 ± 0.3%; range: 0.9-2.0%) and push-off (1.6 ± 0.9%; range: 1.2-2.4%) phases, peak braking (2.4 ± 1.1%; range: 1.6-3.6%), and push-off (1.7 ± 0.9%; range: 1.2-2.2%) forces as well as braking (3.7 ± 2.0%; range: 2.8-5.8%) and push-off (2.1 ± 0.8%; range: 1.3-2.6%) impulses. However, with the exception of braking impulse (P = 0.005), there was no influence of running velocity on asymmetry scores for any of the mechanical variables studied (0.118