Marked effect of Cuscuta on puerarin accumulation in cell cultures of Pueraria tuberosa grown in shake flasks and a bioreactor

Goyal, Shaily; Sharma, Varsha; Ramawat, K. G.

doi:10.1007/s11816-011-0167-2

Cited by 12 publications

(5 citation statements)

References 24 publications

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“…The step gradient solvent program was used as follows: 0-2 min: 85% A and 15% B; 2-5 min: 85% A and 15% B; 5-15 min: 80% A and 20% B; 15-20 min: 50% A and 50% B; 20-30 min: 40% A and 60% B; 30-35 min: 30% A and 70% B; 35-45 min: 20% A and 80% B; 45-48 min: 0% A and 100% B; 48-50 min: 0% A and 100% B; 50-55 min: 85% A and 15% B. Separation was performed at a flow rate of 1.0 mL/min, and chromatographic peaks were monitored at 254 nm [78,79]. The analyses of the samples were run in triplicate; identification is made by comparing the retention times and quantified by using standard curves for the peak area of the isoflavone standards.…”

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Evaluation of Total Isoflavones in Chickpea (Cicer arietinum L.) Sprouts Germinated under Precursors (p-Coumaric Acid and L-Phenylalanine) Supplementation

Arora,

Kanthaliya,

Joshi

et al. 2023

Plants

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Cicer arietinum L. (Bengal gram, chickpea) is one of the major pulse crops and an important part of traditional diets in Asia, Africa, and South America. The present study was conducted to determine the changes in total isoflavones during sprouting (0, 3, and 7 days) along with the effect of two precursor supplementations, p-coumaric acid (p-CA) and L-phenylalanine (Phe), in C. arietinum. It was observed that increasing sprouting time up to the seventh day resulted in ≈1282 mg 100 g−1 isoflavones, which is approximately eight times higher than chickpea seeds. The supplementation of Phe did not affect the total length of sprouts, whereas the supplementation of p-CA resulted in stunted sprouts. On the third day of supplementation with p-CA (250 mg L−1), the increase in the total phenolic content (TPC) (80%), daidzein (152%), and genistin (158%) contents were observed, and further extending the supplementation reduced the growth of sprouts. On the seventh day of supplementation with Phe (500 mg L−1), the increase in TPC by 43% and genistin content by 74% was observed compared with non-treated sprouts; however, the total isoflavones content was found to be 1212 mg 100 g−1. The increased TPC was positively correlated with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (r = 0.787) and ferric-reducing antioxidant potential (FRAP) (r = 0.676) activity. This study suggests that chickpea sprouts enriched in TPC and antioxidants can be produced by the appropriate quantity of precursor supplementation on a particular day. The results indicated major changes in the phytochemical content, especially daidzein and genistin. It was also concluded that the consumption of 100 g of seventh-day sprouts provided eight times higher amounts of isoflavones in comparison to chickpea seeds.

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Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Evaluation of Total Isoflavones in Chickpea (Cicer arietinum L.) Sprouts Germinated under Precursors (p-Coumaric Acid and L-Phenylalanine) Supplementation

Arora,

Kanthaliya,

Joshi

et al. 2023

Plants

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“…The cultures were scaled-up in a 2-l bioreactor where maximum yield of ~91 mg/l of isofl avonoid was recorded, which was about 19 % higher than the control cultures. Cuscuta elicitation increased puerarin content in particular up to 11 mg/l which was 580 % higher than the value recorded in the control cultures (Goyal et al 2011 ). Likewise, in C. trifolia , enhanced yield of stilbenes (~8-fold) was recorded under the infl uence of Cuscuta .…”

Section: Production Through Unorganized/organized Culturesmentioning

confidence: 71%

“…The details of basic cell cultures and optimization of SMs are beyond the scope of this paper and can be found elsewhere (Merillon and Ramawat 2007a , b ). The commercial production of a metabolite is a hard to achieve goal requiring several basic studies of nutrient optimization and production at high level (Goyal and Ramawat 2008a , b ;Goyal et al 2011 ). Different plant cultures may have unusual growth and accumulation requirements and thus may require modifi ed procedures for culture.…”

Section: Production Of Secondary Metabolitesmentioning

confidence: 99%

“…It is well known that upon treatment with elicitors, an array of defense reactions, including the accumulation of a range of plant defensive secondary metabolites in plants or in cell cultures, occurs. Feeding of elicitors has been proven to be an effective way to enhance secondary metabolites in plant cell and tissue cultures (Goyal et al 2011 ). Cell cultures of A. precatorius, C. trifolia, P. tuberose and V. vinifera were treated with elicitors to enhance the production of respective SM.…”

Section: Production Through Unorganized/organized Culturesmentioning

confidence: 99%

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Biodiversity of Lianas

Parthasarathy¹

2015

Sustainable Development and Biodiversity

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This book series provides complete, comprehensive and broad subject based reviews about existing biodiversity of different habitats and conservation strategies in the framework of different technologies, ecosystem diversity, and genetic diversity. The ways by which these resources are used with sustainable management and replenishment are also dealt with. The topics of interest include but are not restricted only to sustainable development of various ecosystems and conservation of hotspots, traditional methods and role of local people, threatened and endangered species, global climate change and effect on biodiversity, invasive species, impact of various activities on biodiversity, biodiversity conservation in sustaining livelihoods and reducing poverty, and technologies available and required. The books in this series will be useful to botanists, environmentalists, marine biologists, policy makers, conservationists, and NGOs working for environment protection.More information about this series at

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“…In this study, 150 mg/L YE was found to be optimal for isoflavonoid production, yielding 10 mg/L isoflavonoids. Another study reported improved isoflavonoid accumulation using Cuscuta reflexa as an elicitor for P. tuberosa cell cultures [ 26 ]. A maximum yield of 91 mg of isoflavonoids was recorded at 1 g/L, which was approximately 19% higher than that in the control cultures.…”

Section: Introductionmentioning

confidence: 99%

Effect of Biotic Elicitors on the Growth, Antioxidant Activity and Metabolites Accumulation in In Vitro Propagated Shoots of Pueraria tuberosa

Kanthaliya

Joshi

Arora

et al. 2023

Plants

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Pueraria tuberosa contains a wide range of bioactive compounds, including polyphenols, alkaloids, and phytosterols, which make it valuable to the pharmaceutical and food industries. Elicitor compounds trigger the defense mechanisms in plants and are widely used to increase the yield of bioactive molecules in in vitro cultures. The present study was conducted to evaluate the effects of different concentrations of biotic elicitors such as yeast extract (YE), pectin (PEC), and alginate (ALG) on growth, antioxidant activity, and metabolite accumulation in in vitro propagated shoots of P. tuberosa. The elicitors applied to shoot cultures of P. tuberosa significantly increased biomass (shoot number, fresh weight, and dry weight), and metabolites such as protein, carbohydrates, chlorophyll, total phenol (TP), and total flavonoid (TF) contents, as well as antioxidant activity compared to untreated control. Biomass, TP, and TF contents, as well as antioxidant activity, were most significant in cultures treated with 100 mg/L PEC. In contrast, chlorophyll, protein, and carbohydrate increased most in cultures treated with 200 mg/L ALG. Application of 100 mg/L of PEC led to the accumulation of high amounts of isoflavonoids including puerarin (220.69 μg/g), daidzin (2935.55 μg/g), genistin (5612 μg/g), daidzein (479.81 μg/g), and biochanin-A (111.511 μg/g) as analyzed by high-performance liquid chromatography (HPLC). Total isoflavonoids content of 100 mg/L PEC treated shoots was obtained as 9359.56 μg/g, 1.68-fold higher than in vitro propagated shoots without elicitors (5573.13 μg/g) and 2.77-fold higher than shoots of the mother plant (3380.17 μg/g). The elicitor concentrations were optimized as 200 mg/L YE, 100 mg/L PEC, and 200 mg/L ALG. Overall, this study showed that the application of different biotic elicitors resulted in better growth, antioxidant activity, and accumulation of metabolites in P. tuberosa, which could lead to obtaining phytopharmaceutical advantages in the future.

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Marked effect of Cuscuta on puerarin accumulation in cell cultures of Pueraria tuberosa grown in shake flasks and a bioreactor

Cited by 12 publications

References 24 publications

Evaluation of Total Isoflavones in Chickpea (Cicer arietinum L.) Sprouts Germinated under Precursors (p-Coumaric Acid and L-Phenylalanine) Supplementation

Evaluation of Total Isoflavones in Chickpea (Cicer arietinum L.) Sprouts Germinated under Precursors (p-Coumaric Acid and L-Phenylalanine) Supplementation

Biodiversity of Lianas

Effect of Biotic Elicitors on the Growth, Antioxidant Activity and Metabolites Accumulation in In Vitro Propagated Shoots of Pueraria tuberosa

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