Marked Inhibition of Cellular Proliferation in the Normal Human Esophageal Epithelial Cells and Human Esophageal Squamous Cancer Cells in Culture by Carotenoids: Role for Prevention and Early Treatment of Esophageal Cancer

Dutta, Sudhir K.; Surapaneni, Balarama K

doi:10.31557/apjcp.2018.19.11.3251

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…Blood carotenoids are a reliable biomarker of usual intake of fruits and vegetables (33,38) . Carotenoids can reduce damage from reactive oxidative species, inhibit lipid peroxidation and are involved in cellular communications that regulate proliferation and apoptosis (39,40) . Inverse associations between dietary or circulating levels of carotenoids and mortality have been previously observed (23,41) , suggesting the potential benefits of carotenoids for optimal health and prevention of disease.…”

Section: Discussionmentioning

confidence: 99%

Association of serum levels of antioxidant micronutrients with mortality in US adults: National Health and Nutrition Examination Survey 1999–2002

Peeri

Chai

Cooney

et al. 2020

Public Health Nutr.

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Objectives: To examine associations between serum antioxidant levels and mortality (all-cause, cancer, and cardiovascular disease (CVD)) among U.S. adults. Design: We examined the risk of death from all-cause and cause-specific mortality associated with serum antioxidant (vitamin E and carotenoids) and vitamin A levels using Cox regression models to estimate hazards ratios (HR) and 95% confidence intervals (CIs). Setting: The National Health and Nutrition Examination Survey (NHANES) 1999-2002 were follow-up through December 31, 2015. Participants: The NHANES 1999-2002 cohort included 8,758 participants aged ≥20 years. Serum carotenoid levels were only assessed for the 1999-2000 cycle. Therefore, sample size for each assessed antioxidant ranged from 4,633 to 8,758. Results: Serum vitamin E level was positively associated with all-cause mortality (HR= 1.22, 95% CI: 1.04, 1.43, highest vs. lowest quartile). No other antioxidants were associated with mortality in overall analysis. In race/ethnicity-specific analyses, high vitamin E and α-tocopherol levels were associated with increased risk of all-cause mortality among non-Hispanic Whites. Among non-Hispanic Blacks, serum α-tocopherol level was associated with decreased risk of cancer mortality (HR= 0.30, 95% CI: 0.12-0.75, third vs. first quartile), and total carotenoids levels with reduced risk of CVD mortality (HR=0.26; 95% CI: 0.07, 0.97, second vs. lowest quartile). Hispanics with high β-carotene levels had reduced risk of CVD mortality. Conclusions: Serum antioxidant levels may be related to mortality; these associations may differ by race/ethnicity and appeared to be non-linear for all-cause and cause-specific mortality. Further studies are needed to confirm our results.

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Section: Discussionmentioning

confidence: 99%

Association of serum levels of antioxidant micronutrients with mortality in US adults: National Health and Nutrition Examination Survey 1999–2002

Peeri

Chai

Cooney

et al. 2020

Public Health Nutr.

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“…Most of the in vitro studies recently focused on anti-carcinogenic mechanism of beta-carotene in lung, liver and blood cells. In some animal studies, alfa-carotene demonstrated a higher suppressor activity on liver, lung, skin and colon carcinogenesis compared to beta-carotene ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that the molecular protective mechanisms of carotenoids in isolated human cell culture include: ( 1 ) stopping the cell cycle in G1/G0 phase by decreasing cycline D1 levels; ( 2 ) apoptosis induction downregulating survivin levels; ( 3 ) increase in cellular gap junction communication and ( 4 ) angiogenic effect through modulation of various cytokines including decreased interleukin-6 (IL-6), IL-1b, tumour necrosis factor alpha and granulocyte-macrophage colony-stimulating factor levels and increased IL-2 and TIMP metallopeptidase inhibitor 1 (TIMP-1) levels ( 30 , 31 ). Chemotherapeutic effects via similar mechanisms have been reported for all-trans retinoic acid (ATRA), another vitamin A analog ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

“…Dutta et al ( 30 ) showed that alfa and beta-carotene, in 5 and 10 µM doses respectively, synergistically decreased cell proliferation and DNA synthesis in oesophagus epithelial cell culture (HEE) and squamous cancer cell culture (HESC) when used in combination and identified that early administration could be of benefit in oesophagus cancer treatment, for example in Barret oesophagus, and a lower dose of beta-carotene could be synergistically used with alfa-carotene for protection against oesophagus malignancy.…”

Section: Discussionmentioning

confidence: 99%

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The comparison of pegylated liposomal doxorubicin and beta-carotene effects on JAR and JEG-3 choriocarcinoma human cell culture models

Erol¹,

Sel²,

Harma³

et al. 2020

J Turkish German Gynecol Assoc

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Objective: The aim was to investigate the effectiveness of pegylated liposomal doxorubicin (PLD), beta-carotene, and a combination of PLD and beta-carotene on JAR and JEG-3 human choriocarcinoma (CC) cell lines for the treatment of CC. Material and Methods: JAR and JEG-3 cells were cultured. PLD and beta-carotene trial groups were determined with different doses (for single drug trial; PLD 1, 2, 5 μg/mL and beta-carotene 1, 5, 10 μg/mL, and for combined drug trial; all PLD doses combined with beta-carotene 5 μg/mL). Drugs were administered to cultures simultaneously, and 72 hours later the cells were detached using trypsin-ethylenediamine tetraacetic acid solution. The percentage of apoptotic cells was determined by flow cytometry after annexin V staining. One set of the supernatant was collected before trypsin application to investigate beta-human chorionic gonadotropin (β-hCG) and hyperglycosylated hCG (H-hCG) levels. Statistical analyses of the apoptotic ratios were performed using Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney U tests. Results: Apoptosis increased in JAR and JEG-3 cultures after treatment with all doses of PLD (p<0.05). A single application of each beta-carotene dose increased apoptosis in JAR cells (p<0.05) but had no apoptotic effects on JEG-3 cells. In the PLD and beta-carotene combination group, apoptosis increased in both JAR and JEG-3 cells (p<0.05). Conclusion: To our knowledge, this is the first investigation of the effectiveness of PLD, beta-carotene, and PLD + beta-carotene combination therapy in two different CC cell lines. PLD is a promising chemotherapeutic drug, and beta-carotene can be used as a novel non-chemotherapeutic agent for treatment of CC. Based on the results of this study, vitamin A supplementation may have promise as a preventive measure. However, these data need support from animal experiments and clinical trials.

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“…Other mechanisms underlying the anticancer effects of this carotenoid in esophageal squamous carcinoma cells include up-regulation of PPAR-γ and down-regulation of cyclin D1 and COX-2 expression [384]. Likewise, β-carotene, in combination with α-carotene, demonstrated a strong antiproliferative activity as well as a reduction in DNA synthesis in esophageal cancer cells [385]. In relation to hepatic cancer, a mixture of different carotenoids, including αand β-carotene, lycopene, LUT, and BCX, evidenced a higher antimetastatic activity than individual carotenoids in human hepatocarcinoma SK-Hep-1 cells [386].…”

Section: Human Studiesmentioning

confidence: 99%

Anti-Inflammatory and Anticancer Effects of Microalgal Carotenoids

et al. 2021

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Acute inflammation is a key component of the immune system’s response to pathogens, toxic agents, or tissue injury, involving the stimulation of defense mechanisms aimed to removing pathogenic factors and restoring tissue homeostasis. However, uncontrolled acute inflammatory response may lead to chronic inflammation, which is involved in the development of many diseases, including cancer. Nowadays, the need to find new potential therapeutic compounds has raised the worldwide scientific interest to study the marine environment. Specifically, microalgae are considered rich sources of bioactive molecules, such as carotenoids, which are natural isoprenoid pigments with important beneficial effects for health due to their biological activities. Carotenoids are essential nutrients for mammals, but they are unable to synthesize them; instead, a dietary intake of these compounds is required. Carotenoids are classified as carotenes (hydrocarbon carotenoids), such as α- and β-carotene, and xanthophylls (oxygenate derivatives) including zeaxanthin, astaxanthin, fucoxanthin, lutein, α- and β-cryptoxanthin, and canthaxanthin. This review summarizes the present up-to-date knowledge of the anti-inflammatory and anticancer activities of microalgal carotenoids both in vitro and in vivo, as well as the latest status of human studies for their potential use in prevention and treatment of inflammatory diseases and cancer.

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Marked Inhibition of Cellular Proliferation in the Normal Human Esophageal Epithelial Cells and Human Esophageal Squamous Cancer Cells in Culture by Carotenoids: Role for Prevention and Early Treatment of Esophageal Cancer

Cited by 7 publications

References 26 publications

Association of serum levels of antioxidant micronutrients with mortality in US adults: National Health and Nutrition Examination Survey 1999–2002

Association of serum levels of antioxidant micronutrients with mortality in US adults: National Health and Nutrition Examination Survey 1999–2002

The comparison of pegylated liposomal doxorubicin and beta-carotene effects on JAR and JEG-3 choriocarcinoma human cell culture models

Anti-Inflammatory and Anticancer Effects of Microalgal Carotenoids

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