Marked seasonality of aerobic anoxygenic phototrophic bacteria in the coastal <scp>NW M</scp>editerranean <scp>S</scp>ea as revealed by cell abundance, pigment concentration and pyrosequencing of <i>puf<scp>M</scp></i> gene

Ferrera, Isabel; Borrego, Carles M.; Salazar, Guillem; Gasol, Josep M.

doi:10.1111/1462-2920.12278

Cited by 61 publications

(98 citation statements)

References 70 publications

(157 reference statements)

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“…6). These findings are in agreement with results reported by Ferrera et al (2014), who found a defined seasonal pattern with summer maxima and winter minima both in AAP abundance and BChl a concentration. However, it must be kept in mind that we have presented BChl a data and not AAP abundances and therefore only tentative extrapolations can be made to describe AAP distribution considering that (1) per cell concentration of BChl a can be very variable and (2) a previous study in the Mediterranean Sea showed a highly significant correlation between pigment concentration and cell numbers (Lamy et al 2011).…”

Section: Dynamics Of Pigments Relevant To Photoheterotrophysupporting

confidence: 94%

“…Prokaryotic photoheterotrophs are divided into 3 main groups: aerobic anoxygenic phototrophic bacteria (AAPs), proteorhodopsin-containing bacteria (PR) and aerobic oxygenic phototrophic cyanobacteria dis playing heterotrophic-like behaviour (Béjà & Suzuki 2008). AAPs are known to generally represent less than 10% (though occasionally up to 25%; Lami et al 2007) of total prokaryotes in the marine photic layer (Lamy et al 2011, Ferrera et al 2014, PR have been estimated to represent up to 50% of the microorganisms living in the N orth Atlantic Ocean (Campbell et al 2008), and the contribution of Prochlorococcus to leucine uptake in the North Atlantic has been found to be up to 25% within the whole prokaryotic community (Mary et al 2008). Given the importance of these metabolic strategies, our view of carbon fluxes has dramatically changed over the past 15 yr (Gasol et al 2008, Ruiz-González et al 2013.…”

Section: Introductionmentioning

confidence: 99%

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Effect of sunlight on prokaryotic organic carbon uptake and dynamics of pigments relevant to photoheterotrophy in the Adriatic Sea

Celussi¹,

Gallina²,

Ras³

et al. 2015

Aquat. Microb. Ecol.

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Marine photoheterotrophic microorganisms are capable of using light to meet their energy requirements and organic compounds as both carbon and energy sources. We still have little knowledge of the extent to which stimulation of these microorganisms by light could affect the estimates of organic carbon uptake. We evaluated the light and dark prokaryotic organic carbon uptake ( 3 H-leucine) rates in a grid of stations covering the whole Adriatic Sea during winter and late summer. Light-exposed (photosynthetically active radiation and ultraviolet radiation [UVR]) surface samples were either unaffected, photostimulated or photoinhibited without any clear geographical or seasonal pattern. Light-enhanced leucine uptake occurred only in 23% of assays, suggesting that photoheterotrophy is not a major metabolic strategy and/or it is often counterbalanced by negative effects caused by UVR. Concentrations of bacteriochlorophyll a, zeaxanthin and divinyl chlorophyll a were measured in order to relate 3 H-leucine uptake to the distribution of aerobic anoxygenic photo trophs (AAPs), total Cyanobacteria and Prochlorococcus populations, respectively, together with direct estimates of total prokaryotes and Synechococcus abundance. No relationship between light-enhanced leucine uptake and presence of picocyanobacteria or AAPs was evident. Divinyl chlorophyll a concentration was below the limit of detection in February, whereas in late summer the highest values were found around 50 m depth in the central and southern basins. In contrast, bacteriochlorophyll a concentration was correlated to total prokaryote abundance and dissolved organic carbon. Since locally leucine uptake in the light was markedly different from dark controls, in situ light incubations should be more appropriate than dark incubations when carbon budget calculations are intended.KEY WORDS: Prokaryotic production · Bacteriochlorophyll a · Divinyl chlorophyll a · Zeaxanthin · Light Resale or republication not permitted without written consent of the publisher

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Section: Dynamics Of Pigments Relevant To Photoheterotrophysupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Effect of sunlight on prokaryotic organic carbon uptake and dynamics of pigments relevant to photoheterotrophy in the Adriatic Sea

Celussi¹,

Gallina²,

Ras³

et al. 2015

Aquat. Microb. Ecol.

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“…The ecological and biogeochemical implications of photoheterotrophic pathways are receiving increasing attention, and during the past decade there have been remarkable advances in our understanding of abundance and distribution patterns of AAP bacteria in lakes and oceans Ferrera et al, 2013;Fauteux et al, 2015). One of the main challenges in furthering our understanding of the ecological role of this group, and in particular in determining the scenarios where they may have an ecological advantage over their heterotrophic bacterial counterparts, has been determining the actual activity of these bacteria in situ.…”

Section: Discussionmentioning

confidence: 99%

Single-cell activity of freshwater aerobic anoxygenic phototrophic bacteria and their contribution to biomass production

et al. 2016

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Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophs that despite their low abundances have been hypothesized to play an ecologically and biogeochemically important role in aquatic systems. Characterizing this role requires a better understanding of the in situ dynamics and activity of AAP bacteria. Here we provide the first assessment of the single-cell activity of freshwater AAP bacteria and their contribution to total bacterial production across lakes spanning a wide trophic gradient, and explore the role of light in regulating AAP activity. The proportion of cells that were active in leucine incorporation and the level of activity per cell were consistently higher for AAP than for bulk bacteria across lakes. As a result, AAP bacteria contributed disproportionately more to total bacterial production than to total bacterial abundance. Interestingly, although environmentally driven patterns in activity did not seem to differ largely between AAP and bulk bacteria, their response to light did, and exposure to light resulted in increases in the proportion of active AAP bacteria with no clear effect on their cell-specific activity. This suggests that light may play a role in the activation of AAP bacteria, enabling these photoheterotrophs to contribute more to the carbon cycle than suggested by their abundance.

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“…Light availability would explain why AAP bacterial abundance is highest in the euphotic zone of the oceans and decreases with depth (Cottrell et al, 2006;Sieracki et al, 2006;Salka et al, 2008). Abundance of these bacteria also correlated with light intensity and day length over a year in Mediterranean coastal waters (Ferrera et al, 2014). We hypothesized that AAP bacteria would be more active than the rest of the bacterial community and that their activity would be enhanced by light.…”

Section: Introductionmentioning

confidence: 98%

Leucine incorporation by aerobic anoxygenic phototrophic bacteria in the Delaware estuary

2014

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Aerobic anoxygenic phototrophic (AAP) bacteria are well known to be abundant in estuaries, coastal regions and in the open ocean, but little is known about their activity in any aquatic ecosystem. To explore the activity of AAP bacteria in the Delaware estuary and coastal waters, single-cell 3 H-leucine incorporation by these bacteria was examined with a new approach that combines infrared epifluorescence microscopy and microautoradiography. The approach was used on samples from the Delaware coast from August through December and on transects through the Delaware estuary in August and November 2011. The percent of active AAP bacteria was up to twofold higher than the percentage of active cells in the rest of the bacterial community in the estuary. Likewise, the silver grain area around active AAP bacteria in microautoradiography preparations was larger than the area around cells in the rest of the bacterial community, indicating higher rates of leucine consumption by AAP bacteria. The cell size of AAP bacteria was 50% bigger than the size of other bacteria, about the same difference on average as measured for activity. The abundance of AAP bacteria was negatively correlated and their activity positively correlated with light availability in the water column, although light did not affect 3 H-leucine incorporation in light-dark experiments. Our results suggest that AAP bacteria are bigger and more active than other bacteria, and likely contribute more to organic carbon fluxes than indicated by their abundance.

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Marked seasonality of aerobic anoxygenic phototrophic bacteria in the coastal NW Mediterranean Sea as revealed by cell abundance, pigment concentration and pyrosequencing of pufM gene

Cited by 61 publications

References 70 publications

Effect of sunlight on prokaryotic organic carbon uptake and dynamics of pigments relevant to photoheterotrophy in the Adriatic Sea

Effect of sunlight on prokaryotic organic carbon uptake and dynamics of pigments relevant to photoheterotrophy in the Adriatic Sea

Single-cell activity of freshwater aerobic anoxygenic phototrophic bacteria and their contribution to biomass production

Leucine incorporation by aerobic anoxygenic phototrophic bacteria in the Delaware estuary

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