Marker-assisted breeding of a LOX-3-null rice line with improved storability and resistance to preharvest sprouting

Abstract

Breakage of the tight linkage between rice seed lipoxygenase - 3 and easy preharvest sprouting trait led to breeding of lines with few stale flavors after long storage and desirable preharvest sprouting resistance. Lipoxygenase-3 (LOX-3) is involved in the production of volatile constituents in stored rice, and the development of stale flavor is delayed in LOX-3 null rice. In the process of breeding new LOX-3-null lines with long storability, we found a close association between LOX-3 and preharvest sprouting resistance. To determine whether this relationship was due to the tight linkage of two genes or the pleiotropic effect of LOX-3, we performed marker-assisted selection using a BC3F3 population derived from crosses between LOX-3-present/preharvest sprouting-resistant lines and LOX-3-null/preharvest susceptible lines. In one individual, a recombination event occurred 13 kb downstream of LOX-3 (RM15750) and a significant quantitative trait locus, namely qPHS3, for easy preharvest sprouting trait (LOD = 10.4) was detected in an 842-kb region between RM15711 and RM15768. Using BC3F4 and BC3F5 populations, we succeeded in selecting LOX-3-absent and preharvest sprouting-resistant lines with only a 393-kb introgressed chromosome segment from the donor line for LOX-3-null at the LOX-3 locus on chromosome 3. This result indicated that the LOX-3 gene and the locus affecting preharvest sprouting are distinct. The selected line was named 'Hokuriku 244'. Sensory testing of rice grains with and without LOX-3 confirmed that stale flavor production in LOX-3-null rice during storage was lower than in normal LOX-3 rice. These results indicated that rice varieties with little stale flavor after long storage and preharvest sprouting resistance had been selected.

Identification and characterization of the rice pre‐harvest sprouting mutants involved in molybdenum cofactor biosynthesis

“…Pre‐harvest sprouting (PHS) or vivipary is a negative trait for cereal production, because of the resulting poor grain yield and quality (Suzuki et al ., ; Shorinola et al ., ). ABA plays a significant role in plant growth and development, particularly in primary dormancy and seed germination (Koornneef et al ., ; Gubler et al ., ; Estelle, ; Pennisi, ; Ping et al ., ; Verma et al ., ).…”

Characterization key genes of Arabidopsis seedlings in response to β-caryophyllene, eugenol using combined transcriptome and WGCN analysis

“…Lipoxygenase (LOX) is responsible for catalyzing the peroxidation of polyunsaturated fatty acids ( Suzuki et al., 2015 ). In Petunia hybrida, PhCS plays a crucial role in the synthesis of chlorophylls, carotenoids, and anthocyanins ( Masson et al., 2019 ).…”

Genetic Dissection of Seed Dormancy in Rice (Oryza sativa L.) by Using Two Mapping Populations Derived from Common Parents

“…For qSD3.2 , eight out of seventeen putative genes in its peak bin (approximately 268 kb) can be considered putative candidates, as they were specifically and highly expressed in the seeds and significantly induced or repressed by ABA. Among these genes, three genes located in the peak bin of qSD3.2 , including the preharvest sprouting locus qPHS3 reported in a previous study (Suzuki et al 2015 ), a lipoxygenase gene ( OsLOX3 ) associated with seed longevity (Xu et al 2015 ) and an ethylene-related gene (LOC_Os03g49400) are of interest. It has been reported that ethylene insensitive2 , a homologous gene of LOC_Os03g49400, can increase ABA sensitivity during seed germination in Arabidopsis (Wang et al 2007 ).…”