Marker-assisted combination of major genes for pathogen resistance in potato

Gebhardt, Christiane; Bellin, Diana; Henselewski, Heike; Lehmann, Wolf D.; Schwarzfischer, J.; Valkonen, Jari P. T.

doi:10.1007/s00122-006-0248-8

Cited by 159 publications

(103 citation statements)

References 34 publications

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“…A gene (Nx phu ) for resistance to PVX was mapped in the cultivated potato species S. phureja (Tommiska et al 1998). Reliable polymerase chain reaction (PCR) based markers were developed for Ry adg (Sorri et al, 1999, Kasai et al 2000 and are now used by potato breeders (e.g., Gebhardt et al 2006, Rizza et al 2006. Another gene, Ry sto , that also confers extreme resistance to all strains of PVY was studied in potato materials originating in the International Potato Center (CIP), Peru, and PCR-based markers were developed for use in breeding programmes .…”

Section: Potato Biotechnologymentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

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Plants bind solar energy to organic matter via photosynthesis and assimilation of carbon dioxide from the atmosphere and comprise the major source of nutrition and bioenergy. Plant biotechnology contributes to solution of important constraints in food and feed production and creates new technologies and applications for the sustainable use of plant resources. Genome-wide approaches such as massive parallel sequencing and microarrays to study gene expression, molecular markers for selection of important traits in breeding, characterization of genetic diversity with the aforementioned approaches, and somatic hybridization and genetic transformation are important tools in plant biotechnology. In this paper, studies carried out on enhanced resistance to viruses and tolerance of cold stress in potato, genetic modification of flower pigmentation and morphology in gerbera, production of edible vaccines in transgenic barley seeds, and expression of heterologous proteins for pharmaceutical purposes from vector viruses were chosen to exemplify the general utility of biotechnological approaches and also how plant biotechnology research has developed on cultivated plants at University of Helsinki. The studies reveal cellular and genetic mechanisms and provide scientific information that can be used for widening the uses of crop plants. They can also be used to detect any putative risks associated with the use of the biotechnological application in agriculture and horticulture and to develop practises which reduce any inadvertent negative consequences that plant production may have to the environment.

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Section: Potato Biotechnologymentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

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“…За последние два десятилетия для картофеля разработаны многочисленные ДНК-маркеры, позволя-ющие проводить маркер-опосредованный отбор (MAS) перспективных генотипов (см. обзоры (Simko et al, 2007;Tiwari et al, 2012Tiwari et al, , 2013Gebhardt, 2013;Ramakrishnan et al, 2015)) и широко применяющиеся в молекулярном скрининге исследователями разных стран (Flis et al, 2005;Gebhardt et al, 2006;Milczarek et al, 2011;Mori et al, 2011Mori et al, , 2012Asano et al, 2012;Schultz et al, 2012;Бирюкова и др., 2015;Антонова и др., 2016;Asano, Tamiya, 2016;Зотеева и др., 2017;Клименко и др., 2017).…”

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Molecular Screening of Potato Varieties Bred in the Northwestern Zone of the Russian Federаtion

Гавриленко¹,

Klimenko²,

Antonova³

et al. 2018

Vestn. VOGiS

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Increasing the resistance of varieties to diseases and pests is a priority in potato breeding. Many potato varieties created in the Leningrad Scientific Research Institute of Agriculture "Belogorka" are multi-species hybrids possessing complex resistance to the most spread pathogens in combination with a high plasticity, which determined their zoning in many regions of the Russian Federation. Potato varieties and hybrids created in different periods of time in this institute and in the Ltd Breeding company "LiGa" were included in marker-assisted selection with 22 markers of 14 R genes conferring extreme resistance to viruses PVY and PVX, resistance to cyst nematodes Globodera pallida (patotypes Pa2, Pa3) and G. rostochiensis (pathotype Ro1) and resistance to late blight. The data of molecular screening were compared with the results of the State Test of Varieties for Disease Resistance, as well as with other published sources. Markers of sterile and fertile cytoplasm types were also used in the study. Based on the results of molecular screening, varieties and hybrids with different combinations of markers of R genes conferring resistance to the most harmful pathogens (Ry sto , Ryf sto , Rx1, Rx2, Gro14, H1, Gpa2, R1, R3a, Rpiblb1, Rpisto1) were detected. Among them, genotypes with sterile D-, T-, W/gamma cytoplasmic types were selected. Analysis of their pollen fertility, crossability and pedigree data showed that breeders succeed in obtaining fertile forms with the D-and Задача повышения устойчивости сортов к болезням и вредителям является приоритетной для селекции картофеля. Многие сорта, созданные селекционерами северо-западной зоны Российской Федерации, являются многовидовыми гибридами, обладают ком-плексной устойчивостью к наиболее вредоносным патогенам в сочетании с высокой пластичностью, о чем свидетельствует их районирование в разных регионах нашей страны. В статье пред-ставлены результаты молекулярного скрининга сортов и гибридов, созданных в разные годы в ЛенНИИСХ «Белогорка» и в ООО Селек-ционная фирма «ЛиГа». Молекулярный скрининг проведен с ис-пользованием 22 маркеров 14 R-генов устойчивости к вирусам PVY и PVX, к цистообразующим нематодам -Globodera pallida (патотипы Pa2, Ра3) и G. rostochiensis (патотип Ro1) и к фитофторозу. Данные молекулярно-генетического анализа сопоставлялись с результа-тами Государственного испытания сортов на устойчивость к бо-лезням и с другими опубликованными источниками. В работе ис-пользованы также маркеры стерильных и фертильных типов цито-плазм. По результатам молекулярного скрининга выделены сорта и гибриды c разными сочетаниями маркеров R-генов (Ry sto , Ryf sto , Rx1, Rx2, Gro14, H1, Gpa2, R1, R3a, Rpiblb1, Rpisto1), обладающие D-, T-, W/gamma стерильными типами цитоплазм. Анализ фертиль-но сти пыльцы, результатов скрещиваний и данных родословных показал, что селекционерам удалось получить фертильные формы с D-и Т-типами цитоплазм, несущие генетический материал мекси-канских полиплоидных видов Solanum stoloniferum и S. demissum. Информация о наличии у гибридов и с...

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“…These represent a 95% correlation between molecular and serological results. Both methods are usually applied as a tool for virus resistances evaluations; like Gebhardt et al (2006), Sagredo et al (2009), andOrtega andLopez-Vizcon (2012). The remaining 148 accessions showed no virus infection and no molecular marker amplification, task that might be explained by unknown PVY resistance sources, since that plant has been exposed to natural pest presence on the field.…”

Section: Discussionmentioning

confidence: 99%

“…Bendahmane et al (1997) localized the Rx1 gene on chromosome XII, using the GP34 and CP60 molecular markers. The CP60 marker has been used by other authors such as Gebhardt et al (2006) and Veramendi et al (2011). Comparing the same accessions used in this investigation that showed amplification using the CP60 marker, with previous evaluations of the Potato Genebank, Contreras and Banse (1982) reported that accessions 548-UA-1328, 464-UA-1360, and 186-UA-1045 are not resistant to PVX as determined by serological evaluation.…”

Section: Discussionmentioning

confidence: 99%

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Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile

López

Riegel

Lizana

et al. 2015

Chilean J. Agric. Res.

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Potato Genebank of the Universidad Austral de Chile (UACh) is an important gene bank in Chile. The accessions collected all over the country possess high genetic diversity, present interesting agronomic and cooking traits, and show resistance to biotic and abiotic stress. A particularly interesting subgroup of the gene bank includes the accessions collected in the South of Chile, the Chilota Potato Genebank. The focus of this study is the identification of virus and nematode resistant genes in potatoes (Solanum tuberosum L.), using the RYSC3 and YES3-3B molecular markers. The Potato virus Y (PVY) resistance genes Ry adg and Ry sto were identified. Furthermore, the CP60 marker was used to assess the Rx resistance gene that confers resistance to Potato virus X (PVX). In addition, the HC and GRO1-4 markers were utilized to identify the GpaV vrn _QTL and Gro1-4, resistance genes of Globodera pallida and Globodera rostochiensis, respectively. Both G. pallida and G. rostochiensis are Potato Cyst Nematodes (PCN). The plant material used in this study included leaves from 271 accessions of the gene bank. These samples were collected in the field where natural pathogen pressure of potential viruses and diseases exists. ELISA assays were run for field detection of PVY and PVX. However, there have been no previous reports of nematode presence in the plant material. The results herein presented indicate presence of virus and nematode resistance genes in accessions of the Chilota Potato Genebank. In terms of virus resistance, 99 accessions out of the 271 tested possess the Ry adg resistance gene and 17 accessions of these 271 tested have the Ry sto resistance gene. Also, 10 accessions showed positive amplification of the Rx1 resistant gene marker. As to nematode resistance, 99 accessions have possible resistance to G. pallida and 54 accessions show potential resistance to G. rostochiensis as detected using the available molecular markers.

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Marker-assisted combination of major genes for pathogen resistance in potato

Cited by 159 publications

References 34 publications

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Molecular Screening of Potato Varieties Bred in the Northwestern Zone of the Russian Federаtion

Identification of virus and nematode resistance genes in the Chilota Potato Genebank of the Universidad Austral de Chile

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