Marker-Assisted Development of a Blue-Grained Substitution Line Carrying the Thinopyrum ponticum Chromosome 4Th(4D) in the Spring Bread Wheat Saratovskaya 29 Background

Abstract

There is growing interest in cereals with anthocyanins in grain as a source of natural biologically active compounds beneficial for human health. In bread wheat, anthocyanins accumulate in the pericarp, under control of Pp genes, and in the aleurone layer, under control of Ba. Breeding anthocyanin-rich wheat cultivars is possible through the transfer of genes from genetic stocks to the desired cultivars. A blue-grained substitution line, s:S294Th(4D) (BC7 progeny), of the bread wheat cultivar Saratovskaya 29 (S29) carrying the Thinopyrum ponticum (Podp.) chromosome 4Th was developed. The 4Th/4D substitution was confirmed with chromosome C-banding and multicolor FISH, as well as by microsatellite analysis. Total anthocyanin content in the bran fraction of the new blue-grained line was 475.7 μg/g compared to 355.6 μg/g of the control purple-grained near-isogenic line, i:S29Pp-A1Pp-D1Pp3P, and a total absence in S29. Although the developed line carries entire chromosome substitution, its 1000 grains weight, milling parameters, and dough physical properties did not differ or decreased slightly comparison to S29. These results support that the developed substitution line can be of interest in breeding programs to increase the anthocyanin production in commercial varieties.

Antioxidant Capacity and Profiles of Phenolic Acids in Various Genotypes of Purple Wheat

“…The anthocyanin pigmentation in the aleurone layer of the grains was a substituted blue-grain wheat line based on the Saratovskaya 29 variety, carrying the Thinopyrum ponticum (Podp., syn. Agropyron elongatum Host., Elytrigia pontica Podp., Holub) chromosome 4Th instead of chromosome 4D (s:S29_4Th/4D), containing the dominant allele of the Ba gene (Blue aleuron) [ 23 ]. A two-stage crossing was carried out with recurrent promising varieties of Siberian breeding and breeding line BW 49880.…”

Phytochemical Analysis of Phenolics, Sterols, and Terpenes in Colored Wheat Grains by Liquid Chromatography with Tandem Mass Spectrometry

“…Saratovskaya 29 (S29). Blue grains were represented by two wheat-wheatgrass substitution lines S29 BLUE(4Th-4B) and S29 BLUE (4Th-4D) developed in the S29 background but carrying Ba gene-containing wheatgrass chromosome 4Th, which replaced wheat chromosomes 4B and 4D, respectively [25,26]. Black grains were represented by four lines, two of them-S29 BLACK (4Th-4B) and S29 BLACK (4Th-4D)-have been developed previously in the S29 background by crossing the above-mentioned lines with purple-grained near-isogenic wheat line S29 PURPLE Pp-D1Pp3 carrying introgressions in chromosomes 7D and 2A, onto which the dominant alleles of genes Pp-D1 and Pp3, respectively, have been mapped [47,48].…”

“…Two blue-grained lines-S29 BLUE (4Th-4B) and S29 BLUE (4Th-4D)are substitution lines where chromosomes 4B and 4D are substituted with Th. ponticum chromosome 4Th carrying the Ba gene determining the blue pigmentation of grains [25,26]. Two deep-purple-grained, i.e., almost black-grained lines-S29 BLACK (4Th-4B) and S29 BLACK (4Th-4D)-in addition to chromosomes 4B and 4D substituted with 4Th, feature introgressions in chromosomes 7D and 2A, where the dominant alleles of genes Pp-D1 and Pp3, respectively, are located [25].…”

“…ponticum chromosome 4Th carrying the Ba gene determining the blue pigmentation of grains [25,26]. Two deep-purple-grained, i.e., almost black-grained lines-S29 BLACK (4Th-4B) and S29 BLACK (4Th-4D)-in addition to chromosomes 4B and 4D substituted with 4Th, feature introgressions in chromosomes 7D and 2A, where the dominant alleles of genes Pp-D1 and Pp3, respectively, are located [25]. Two other black-grained lines-BW BLACK (4Th-4D) and E22 BLACK (4Th-4D)-were developed in the genetic background of breeding line BW49880 (BW) and cv.…”

Wheat, Barley, and Oat Breeding for Health Benefit Components in Grain

“…MAS also demonstrated its efficacy in creating barley with certain alleles of anthocyanin regulatory genes [18]. For breeding blue-grained wheat, besides molecular markers, FISH or C-banding are needed since the Ba gene is alien for wheat and can be inherited from wheat lines with either 4B or 4D chromosome substituted by the Thinopyrum ponticum chromosome 4 [96,97]. Unlike bread wheat, barley has its own Ba gene.…”