Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated <scp>MR219</scp> rice variety

Miah, Gous; Rafii, Mohd Y.; Ismail, Mohd Razi; Puteh, Adam; Harun, Abdul Rahim; Latif, Mohammad Abdul

doi:10.1002/jsfa.8109

Cited by 27 publications

(28 citation statements)

References 45 publications

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“…The recurrent parent was Putra-1, a high yielding rice variety that is resistant to blast disease with genetic background of three blast R-genes, namely; Piz, Pi2, Pi9. Putra-1 variety was developed at the Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia, from a cross between MR219 × Pongsu Seribu 1 [13]. Putra-1 was used as the female parent to hybridize the male parent IRBB60 and F 1 plants were developed.…”

Section: Genetic Materials and Methods Of Plant Breedingmentioning

confidence: 99%

Marker-assisted introgression of multiple resistance genes confers broad spectrum resistance against bacterial leaf blight and blast diseases in Putra-1 rice variety

Rafii

Chukwu

Ramlee

et al. 2019

Preprint

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This experiment was conducted with the aim of introgressing multiple resistance genes against bacterial leaf blight (BLB) and blast diseases through marker-assisted backcross breeding. Two dominant (Xa4 and Xa21) and two recessive (xa5 and xa13) BLB resistance genes were introgressed into a Malaysian elite rice variety Putra-1 with genetic background of three blast resistance (Piz, Pi2 and Pi9) genes and high yielding. Eight polymorphic tightly linked functional and SSR markers were used for foreground selection of target genes. 79 polymorphic SSR markers were used in background selection. The plants were challenged at initial stage of breeding and challenged again at BC 2 F 2 with the most virulent Malaysian pathotypes of Xoo (P7.7) and Magnaporthe oryzae (P7.2) to test their resistance. Results obtained from foreground marker analysis showed that the BC 1 F 1 and BC 2 F 1 both fitted into the Mendel's single gene segregation ratio of 1:1 for both Xoo and blast resistance. At BC 2 F 2 , result obtained indicated that foreground marker segregation fitted into the expected Mendelian ratio of 1:2:1 for blast resistance only. Marker-assisted background selection revealed high percentage of recurrent parent genome recovery (95.9%). It was concluded that resistance to Xoo pathotype P7.7 in IRBB60 was neither due to two independent gene action nor epistasis but substantially due to single nuclear gene action. Also, the inheritance of blast resistance in the pyramided lines to pathotype P7.2 was also attributed to single gene action. The incorporation of four bacterial leaf blight and three blast resistance genes (Xa4+xa5+xa13+Xa21+Pi9+Pi2+Piz) in the newly developed lines provides for broad spectrum and durable resistance against the two major diseases studied.

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Section: Genetic Materials and Methods Of Plant Breedingmentioning

confidence: 99%

Marker-assisted introgression of multiple resistance genes confers broad spectrum resistance against bacterial leaf blight and blast diseases in Putra-1 rice variety

Rafii

Chukwu

Ramlee

et al. 2019

Preprint

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“…The recurrent parent was Putra-1, a high yielding rice variety that is resistant to blast disease with genetic background of three blast R-genes, namely; Piz, Pi2, Pi9. Putra-1 variety was developed at the Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia, Serdang, Malaysia, from a cross between MR219 × PongsuSeribu 1 [13]. Putra-1 was used as the female parent to hybridize the male parent IRBB60 and F 1 plants were developed.…”

Section: Genetic Materials and Backcrossesmentioning

confidence: 99%

“…Phenotypic selection was done by physical observation and selection of plants with desired traits such as high yielding, no symptoms of bacterial leaf blight and blast. Management practices such as planting spacing, weed control, fertilizer application, pest and disease control were done according to the Malaysian Agricultural Research and Development Institute (MARDI) recommended manual [13].…”

Section: Genetic Materials and Backcrossesmentioning

confidence: 99%

Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety

et al. 2019

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Bacterial leaf blight caused by Xanthomonas oryzae pv oryzae (Xoo) and blast caused by Magnaporthe oryzae are major diseases responsible for significant yield loss in rice production across all rice growing regions. Host plant resistance has been advocated as a sustainable means of guarding against the diseases. This experiment was conducted with the aim to introgress multiple resistance genes against bacterial leaf blight and blast diseases through marker-assisted backcross breeding. Two dominant (Xa4 and Xa21) and two recessive (xa5 and xa13) Xoo resistance genes were introgressed into a high yielding Malaysian rice variety Putra-1 with genetic background of three blast resistance (Piz, Pi2 and Pi9) genes. Eight polymorphic tightly linked functional and SSR markers were used for foreground selection of target genes. Seventy nine polymorphic SSR markers were used in background selection. The plants were challenged at initial stage of breeding and challenged again at BC2F2 with the most virulent Malaysian pathotypes of Xoo (P7.7) and Magnaporthe oryzae (P7.2) to test their resistance. Results obtained from foreground marker segregation analysis at BC1F1 and BC2F1 showed that the marker polymorphism both fitted into the Mendel’s single gene segregation ratio of 1:1 for both Xoo and blast resistance. At BC2F2, results indicated that foreground marker polymorphism fitted into the expected Mendelian ratio of 1:2:1 for blast resistance only. Marker-assisted background selection revealed high percentage of recurrent parent genome recovery (95.9%). It was concluded that the inheritance of blast resistance in the introgressed lines was mainly due to single gene action while the inheritance of Xoo resistance was substantially due to single nuclear gene action. The incorporation of four bacterial leaf blight and three blast resistance genes (Xa4 + xa5 + xa13 + Xa21; Pi9 + Pi2 + Piz) in the newly developed lines would provide for broad spectrum and durable resistance against the two major diseases studied.

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“…Accurate repetitive phenotyping in multi-locations and multi-environments under variable growing conditions is required to evaluate the performance and adaptability of the developed MAB products. There have been several examples of introgression of single genes for both biotic and abiotic stresses (gall midge – Das and Rao 2015 ; blast – Miah et al 2016 ; brown plant hopper – Jairin et al 2009 ; submergence – Septiningsih et al 2009 ) in the background of popular high-yielding varieties as well as introgression of more than one gene for biotic stresses ( xa5 + xa13 + Xa21 - Singh et al 2001 , Kottapalli et al 2010 ; Xa21 + xa13 - Singh et al 2011 ) for oligogenic traits controlled by major genes.…”

Section: Introductionmentioning

confidence: 99%

Marker-assisted selection strategy to pyramid two or more QTLs for quantitative trait-grain yield under drought

Kumar

Sandhu

Dixit

et al. 2018

Rice

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BackgroundMarker-assisted breeding will move forward from introgressing single/multiple genes governing a single trait to multiple genes governing multiple traits to combat emerging biotic and abiotic stresses related to climate change and to enhance rice productivity. MAS will need to address concerns about the population size needed to introgress together more than two genes/QTLs. In the present study, grain yield and genotypic data from different generations (F3 to F8) for five marker-assisted breeding programs were analyzed to understand the effectiveness of synergistic effect of phenotyping and genotyping in early generations on selection of better progenies.ResultsBased on class analysis of the QTL combinations, the identified superior QTL classes in F3/BC1F3/BC2F3 generations with positive QTL x QTL and QTL x background interactions that were captured through phenotyping maintained its superiority in yield under non-stress (NS) and reproductive-stage drought stress (RS) across advanced generations in all five studies. The marker-assisted selection breeding strategy combining both genotyping and phenotyping in early generation significantly reduced the number of genotypes to be carried forward. The strategy presented in this study providing genotyping and phenotyping cost savings of 25–68% compared with the traditional marker-assisted selection approach. The QTL classes, Sub1 + qDTY1.1 + qDTY2.1 + qDTY3.1 and Sub1 + qDTY2.1 + qDTY3.1 in Swarna-Sub1, Sub1 + qDTY1.1 + qDTY1.2, Sub1 + qDTY1.1 + qDTY2.2 and Sub1 + qDTY2.2 + qDTY12.1 in IR64-Sub1, qDTY2.2 + qDTY4.1 in Samba Mahsuri, Sub1 + qDTY3.1 + qDTY6.1 + qDTY6.2 and Sub1 + qDTY6.1 + qDTY6.2 in TDK1-Sub1 and qDTY12.1 + qDTY3.1 and qDTY2.2 + qDTY3.1 in MR219 had shown better and consistent performance under NS and RS across generations over other QTL classes.Conclusion“Deployment of this procedure will save time and resources and will allow breeders to focus and advance only germplasm with high probability of improved performance. The identification of superior QTL classes and capture of positive QTL x QTL and QTL x background interactions in early generation and their consistent performance in subsequent generations across five backgrounds supports the efficacy of a combined MAS breeding strategy”.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0227-0) contains supplementary material, which is available to authorized users.

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Marker‐assisted introgression of broad‐spectrum blast resistance genes into the cultivated MR219 rice variety

Cited by 27 publications

References 45 publications

Marker-assisted introgression of multiple resistance genes confers broad spectrum resistance against bacterial leaf blight and blast diseases in Putra-1 rice variety

Marker-assisted introgression of multiple resistance genes confers broad spectrum resistance against bacterial leaf blight and blast diseases in Putra-1 rice variety

Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety

Marker-assisted selection strategy to pyramid two or more QTLs for quantitative trait-grain yield under drought

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