Marker-assisted introgression of the major bacterial blight resistance genes, Xa21 and xa13, and blast resistance gene, Pi54, into the popular rice variety, JGL1798

Swathi, G.; Rani, Ch. V. Durga; Jamaloddin, Mohammed; Madhav, M. S.; Vanisree, S.; Anuradha, Ch.; Kumar, Naveen; Kumari, Kamlesh; Bhogadhi, Sri Chandana; Ramprasad, E.; Sravanthi, P.; Raju, S. Krishnam; Bhuvaneswari, V.; Rajan, C. P. D.; Jagadeeswar, R.

doi:10.1007/s11032-019-0950-2

Cited by 27 publications

(22 citation statements)

References 25 publications

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“…The present study demonstrates successful incorporation of genetic resistance to these diseases using MABC in a popular cold tolerant, long slender grain rice variety Tellahamsa. Marker assisted selection is a widely adapted technique which is being used by several researchers to introgress the genes and it becomes more successful when the markers perfectly linked to the trait of interest are available (JGL1798, Jin 23B hybrids and Swarna Sub1) [35][36][37]. Although there are different resistance sources available for BB, we used ISM (Improved Samba Mahsuri which has Xa21 xa13 and xa5) as donor, since it has good cooking quality and…”

Section: Discussionmentioning

confidence: 99%

“…Genomic DNA was isolated from the leaf samples by following a standard protocol used for plants [27]. The isolated DNA samples were checked for their quality and quantity by using agarose gel electrophoresis (0.8% agarose gel) and spectrophotometer (Thermo electronic corporation UV1) respectively and then were used to set the PCR reactions [35]. The PCR reaction mixture contained 50ng template DNA, 5 picoM each of forward and reverse primers,…”

Section: Dna Isolation and Pcr Amplificationmentioning

confidence: 99%

“…morphological characters and offering a good level of resistance to BB under field conditions in most of the rice growing regions in India. As xa5 is known to exhibit partial dominance and was reported to display negative effects in gene pyramided lines of some Indian rice varieties [35], in the current study we targeted only two genes (xa13 and Xa21) out of three genes (Xa21 xa13 and xa5). ISM has also been used in the earlier molecular breeding studies towards improvement of existing rice varieties [35,38].…”

Section: Plos Onementioning

confidence: 99%

“…Simultaneous step wise back cross transfer approach was used to introgress all the four genes into one genetic background [35]. Two independent back-crossing programs, Tellahamsa × ISM (Cross-I) and Tellahamsa × NLR145 (Cross-II) were started for the development of resistant lines using Tellahamsa as the female and two donor parents (ISM and NLR145) as males.…”

Section: Crossing Scheme and Mabb For Introgression Of Bb And Blast Gmentioning

confidence: 99%

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Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

et al. 2020

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Bacterial blight (BB) and fungal blast diseases are the major biotic constraints that limit rice productivity. To sustain yield improvement in rice, it is necessary to developed yield potential of the rice varieties by incorporation of biotic stress resistance genes. Tellahamsa is a welladapted popular high yielding rice variety in Telangana state, India. However, the variety is highly susceptible to BB and blast. In this study, simultaneous stepwise transfer of genes through marker-assisted backcross breeding (MABB) strategy was used to introgress two major BB (Xa21 and xa13) and two major blast resistance genes (Pi54 and Pi1) into Tellahamsa. In each generation (from F 1 to ICF 3) foreground selection was done using gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13), Pi54MAS (Pi54) and RM224 (Pi1). Two independent BC 2 F 1 lines of Tellahamsa/ISM (Cross-I) and Tellahamsa/NLR145 (Cross-II) possessing 92% and 94% recurrent parent genome (RPG) respectively were intercrossed to develop ICF1-ICF 3 generations. These gene pyramided lines were evaluated for key agro-morphological traits, quality, and resistance against blast at three different hotspot locations as well as BB at two locations. Two ICF 3 gene pyramided lines viz., TH-625-159 and TH-625-491 possessing four genes exhibited a high level of resistance to BB and blast. In the future, these improved Tellahamsa lines could be developed as mega varieties for different agro-climatic zones and also as potential donors for different pre-breeding rice research.

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Section: Discussionmentioning

confidence: 99%

Section: Dna Isolation and Pcr Amplificationmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Crossing Scheme and Mabb For Introgression Of Bb And Blast Gmentioning

confidence: 99%

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Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

et al. 2020

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“…Thus, we considered these five QTLs as stable QTLs. [27] pyramided two bacterial blight resistance genes (xa13 and Xa21) and one bacterial blast resistance gene (Pi54) to improve the resistance to bacterial blight and blast in rice via MAS. The pyramided lines with three genes exhibited higher level of resistance to bacterial blight and blast with better yield than the recurrent parent.…”

Section: Discussionmentioning

confidence: 99%

Identification of a major quantitative trait locus, qGER4.2, conferring resistance to Gibberella ear rot in maize using a Chinese recombination inbred line population

Zhou

et al. 2020

Preprint

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Background: Gibberella ear rot (GER), a prevalent disease caused by Fusarium graminearum, can result in yield loss and mycotoxin contamination in maize. Despite that several QTLs related to GER resistance have been reported previously, few of them was identified in Chinese maize inbred lines. In this study, we employed a Chinese recombination inbred line (RIL) population comprising of 204 lines, developed from a cross involving a resistant parent DH4866 and a susceptible line T877. The population was phenotypically evaluated at three field trial locations under artificial inoculation with F. graminearum and genotyped with an Affymetrix microarray CGMB56K SNP Array to detect the quantitative trait loci (QTLs) for resistance to GER. Results: Based on the genetic linkage map constructed using 1,868 bins as markers, a total of 15 QTLs were identified, and both DH4866 and T877 alleles at these QTLs contributed toward resistance. Of these QTLs, five were stably expressed across multiple locations, including three co-localized with previously reported genomic regions. The largest-effect QTL located on chromosome 4, qGER4.2, which accounted for 5.66~17.10% of the phenotypic variation, was detected at all locations. Based on the phenotypic values of the alleles corresponding to the five stable QTLs, a significant correlation (r2 = 0.45) between resistance to GER and the number of resistant alleles was observed. Conclusions: QTL mapping was successfully employed to identify genetic loci conferring resistance to GER by a high-density genetic map constructed from two Chinese maize germplasms. The five stable QTLs identified, especially the large-effect QTL, qGER4.2, will be useful in maize breeding programs aimed at improving GER resistance.

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Molecular Approaches for Disease Resistance in Rice

et al. 2021

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Rice production needs to be sustained in the coming decades, with changing climatic conditions becoming more conducive to the prevalence of disease outbreaks. Major rice diseases collectively cause enormous economic damage and yield instability. Breeding for disease-resistant rice varieties could be one of the best options to counter these disease outbreaks. Disease-screening protocols and newer technologies are essential for effective phenotyping and would aid in gene discovery and function. Understanding the genetics of disease mechanisms and stacking of broad-spectrum disease-resistance genes could lead to faster development of rice varieties with multiple disease resistance. New molecular breeding approaches are discussed for the development of these varieties. The molecular biology of disease resistance is now better understood and could be well manipulated for improved resilience. Transgenic approaches for disease resistance are discussed. Genome-editing tools for the development of disease-resistant rice varieties are thoroughly discussed. The use of bioinformatics tools to speed up the process and to obtain a better understanding of molecular genetics mechanisms of disease resistance is explained.

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Marker-assisted introgression of the major bacterial blight resistance genes, Xa21 and xa13, and blast resistance gene, Pi54, into the popular rice variety, JGL1798

Cited by 27 publications

References 25 publications

Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

Marker Assisted Gene Pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”

Identification of a major quantitative trait locus, qGER4.2, conferring resistance to Gibberella ear rot in maize using a Chinese recombination inbred line population

Molecular Approaches for Disease Resistance in Rice

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