Marker-assisted pyramiding of bacterial blight and gall midge resistance genes into RPHR-1005, the restorer line of the popular rice hybrid DRRH-3

Kumar, V. Abhilash; Balachiranjeevi, C. H.; Naik, S. Bhaskar; Rekha, G.; Rambabu, R.; Harika, G.; Pranathi, K.; Hajira, S. K.; Anila, M.; Kousik, M. B. V. N.; Kale, Ravindra Ramrao; Kumar, T. Dilip; Prasad, M. S.; Prasad, A.; Padmakumari, A. P.; Laha, G. S.; Balachandran, S. M.; Madhav, M. S.; Senguttuvel, P.; Kemparajau, K. B.; Fiyaz, Abdul R; Bentur, J. S.; Viraktamath, B. C.; Babu, V. Ravindra; Sundaram, R. M.

doi:10.1007/s11032-017-0687-8

Cited by 28 publications

(9 citation statements)

References 32 publications

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“…Similarly, PRR78, an elite restorer line possessing Basmati type grain quality was improved for resistance against BB and blast by Basavaraj et al ( 2010 ) and Singh et al ( 2013 ), respectively. Recently, Balachiranjeevi et al ( 2015 ) and Abhilash Kumar et al ( 2016 ) improved the maintainer line DRR17B and restorer line RPHR-1005, respectively, against BB and blast ( Xa21 + Pi54 ) by transferring a major resistance gene each for BB and blast resistance, implementing the approach similar to that used in the present study. As done in some of our earlier studies (Hari et al, 2011 , 2013 ; Balachiranjeevi et al, 2015 ; Abhilash Kumar et al, 2016 ), in the present study also, molecular markers specific for two major fertility restorer genes (viz., Rf3 and Rf4 ) were deployed in the initial stages of backcrossing in order to obtain complete fertility restorer lines after backcrossing.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Balachiranjeevi et al ( 2015 ) and Abhilash Kumar et al ( 2016 ) improved the maintainer line DRR17B and restorer line RPHR-1005, respectively, against BB and blast ( Xa21 + Pi54 ) by transferring a major resistance gene each for BB and blast resistance, implementing the approach similar to that used in the present study. As done in some of our earlier studies (Hari et al, 2011 , 2013 ; Balachiranjeevi et al, 2015 ; Abhilash Kumar et al, 2016 ), in the present study also, molecular markers specific for two major fertility restorer genes (viz., Rf3 and Rf4 ) were deployed in the initial stages of backcrossing in order to obtain complete fertility restorer lines after backcrossing. Thus, through the present study, improved breeding lines RPHR-1005 possessing tall plant type, excellent resistance against BB and blast, medium-slender grain type along with stable fertility restoration and suitable for use as a restorer parent in three line breeding system have been developed.…”

Section: Discussionmentioning

confidence: 99%

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RETRACTED: Development of Gene-Pyramid Lines of the Elite Restorer Line, RPHR-1005 Possessing Durable Bacterial Blight and Blast Resistance

et al. 2016

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RPHR-1005, the stable restorer line of the popular medium slender (MS) grain type rice hybrid, DRRH-3 was improved in this study for resistance against bacterial blight (BB) and blast diseases through marker-assisted backcross breeding (MABB). In this study, four major resistance genes (i.e., Xa21 and Xa33 for BB resistance and Pi2 and Pi54 for blast resistance) have been transferred to RPHR-1005 using RPBio Patho-1 (possessing Xa21 + Pi2), RPBio Patho-2 (possessing Xa21 + Pi54) and FBR1-15EM (possessing Xa33) as the donors. Foreground selection was carried out using PCR-based molecular markers specific for the target resistance genes and the major fertility restorer genes, Rf3 and Rf4, while background selection was carried out using a set of parental polymorphic rice SSR markers and backcrossing was continued uptoBC2 generation. At BC2F2, plants possessing the gene combination- Xa21 + Pi2, Xa21 + Pi54 and Xa33 in homozygous condition and with >92% recovery of the recurrent parent genome (RPG) were identified and intercrossed to combine all the four resistance genes. Twenty-two homozygous, pyramid lines of RPHR-1005 comprising of three single-gene containing lines, six 2-gene containing lines, eight 3-gene containing lines, and five 4-gene containing lines were identified among the double intercross lines at F3 generation (DICF3). They were then evaluated for their resistance against BB and blast, fertility restoration ability and for key agro-morphological traits. While single gene containing lines were resistant to either BB or blast, the 2-gene, 3-gene, and 4-gene pyramid lines showed good level of resistance against both and/or either of the two diseases. Most of the 2-gene, 3-gene, and 4-gene containing pyramid lines showed yield levels and other key agro-morphological and grain quality traits comparable to the original recurrent parent and showed complete fertility restoration ability, with a few showing higher yield as compared to RPHR-1005. Further, the experimental hybrids derived by crossing the gene-pyramid lines of RPHR-1005 with APMS6A (the female parent of DRRH-3), showed heterosis levels equivalent to or higher than DRRH-3. The results of present study exemplify the utility of MABB for targeted improvement of multiple traits in hybrid rice.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

RETRACTED: Development of Gene-Pyramid Lines of the Elite Restorer Line, RPHR-1005 Possessing Durable Bacterial Blight and Blast Resistance

et al. 2016

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“…Conventional backcross breeding is the primary method used to develop highly BB-resistant rice cultivars, but it cannot accurately transfer multiple genes into the cultivar by phenotypic screening and the process requires a significant amount of time [21,22]. Modified backcross pyramid breeding, combined with molecular marker-assisted selection, has already been demonstrated to increase the precision and efficiency of breeding [23][24][25]. Due to the relatively large amount of work involved with the MAS process, the conventional backcross breeding approach has been widely adopted in breeding programs designed to breed for BB resistance [10,[26][27][28].…”

Section: Discussionmentioning

confidence: 99%

Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Hsu

Chiu²,

Yap

et al. 2020

IJMS

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Tainung82 (TNG82) is one of the most popular japonica varieties in Taiwan due to its relatively high yield and grain quality, however, TNG82 is susceptible to bacterial blight (BB) disease. The most economical and eco-friendly way to control BB disease in japonica is through the utilization of varieties that are resistant to the disease. In order to improve TNG82’s resistance to BB disease, five bacterial blight resistance genes (Xa4, xa5, Xa7, xa13 and Xa21) were derived from a donor parent, IRBB66 and transferred into TNG82 via marker-assisted backcrossing breeding. Five BB-resistant gene-linked markers were integrated into the backcross breeding program in order to identify individuals possessing the five identified BB-resistant genes (Xa4, xa5, Xa7, xa13 and Xa21). The polymorphic markers between the donor and recurrent parent were used for background selection. Plants having maximum contribution from the recurrent parent genome were selected in each generation and crossed with the recipient parent. Selected BC3F1 plants were selfed in order to generate homozygous BC3F2 plants. Nine pyramided plants, possessing all five BB-resistant genes, were obtained. These individuals displayed a high level of resistance against the BB strain, XF89-b. Different BB gene pyramiding lines were also inoculated against the BB pathogen, resulting in more than three gene pyramided lines that exhibited high levels of resistance. The five identified BB gene pyramided lines exhibited yield levels and other desirable agronomic traits, including grain quality and palatability, consistent with TNG82. Bacterial blight-resistant lines possessing the five identified BB genes exhibited not only higher levels of resistance to the disease, but also greater yield levels and grain quality. Pyramiding multiple genes with potential characteristics into a single genotype through marker-assisted selection can improve the efficiency of generating new crop varieties exhibiting disease resistance, as well as other desirable traits.

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“…The Gm4 gene encodes leucine rice repeat domain and exhibit hypersensitive type reaction where death of host cell occurs at the site of insect attack (Bentur et al, 2011;Divya et al, 2015). Pyramiding of GM resistance genes (Gm1, Gm4) and (Gm4, Gm8) was attempted through marker-assisted breeding approach into the elite background of Improved Samba Mahsuri and an elite rice hybrid DRRH3, respectively (Divya et al, 2015;Kumar et al, 2017). In recent years, some efforts have been made to improve some elite rice varieties by marker-assisted pyramiding of various genes conferring to GM, blast, and BLB resistance along with Saltol QTL for salinity tolerance (Das & Rao., 2015;Das, Rao, Varier, Prakash, & Prasad, 2018).…”

Section: Core Ideasmentioning

confidence: 99%

Genomics‐assisted breeding for successful development of multiple‐stress‐tolerant, climate‐smart rice for southern and southeastern Asia

et al. 2021

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Rice (Oryza sativa L.) in rainfed marginal environments is prone to multiple abiotic and biotic stresses, which can occur in combination in a single cropping season and adversely affect rice growth and yield. The present study was undertaken to develop high‐yielding, climate‐resilient rice that can provide tolerance to multiple biotic and abiotic stresses. An assembled first‐crossing scheme was employed to transfer 15 quantitative trait loci (QTL) and genes—qDTY1.1, qDTY2.1, qDTY3.1, qDTY12.1 (drought), Sub1 (submergence), Gm4 (gall midge), Pi9, Pita2 (blast), Bph3, Bph17 (brown plant hoppers), Xa4, xa5, xa13, Xa21, and Xa23 (bacterial leaf blight)—from eight different parents using genomics‐assisted breeding. A funnel mating design was employed to assemble all the targeted QTL and genes into a high‐yielding breeding line IR 91648‐B‐1‐B‐3‐1. Gene‐based linked markers were used in each generation from intercrossing to the F6 generation for tracking the presence of desirable alleles of targeted QTL and genes. Single‐plant selections were performed from F2 onwards to select desirable recombinants possessing alleles of interest with suitable phenotypes. Phenotyping of 95 homozygous F6 lines carrying six to 10 QTL and genes was performed for nonstress, reproductive‐stage (RS) drought, blast, bacterial leaf blight (BLB), gall midge (GM), and for grain quality parameters such as chalkiness, amylose content (AC), gelatinization temperature (GT), and head rice recovery (HRR). Finally, 56 F7 homozygous lines were found promising for multiple‐location evaluation for grain yield (GY) and other traits. These multiple‐stress‐tolerant lines with the desired grain quality profiling can be targeted for varietal release in southern and southeastern Asia through national release systems.

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Marker-assisted pyramiding of bacterial blight and gall midge resistance genes into RPHR-1005, the restorer line of the popular rice hybrid DRRH-3

Cited by 28 publications

References 32 publications

RETRACTED: Development of Gene-Pyramid Lines of the Elite Restorer Line, RPHR-1005 Possessing Durable Bacterial Blight and Blast Resistance

RETRACTED: Development of Gene-Pyramid Lines of the Elite Restorer Line, RPHR-1005 Possessing Durable Bacterial Blight and Blast Resistance

Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Genomics‐assisted breeding for successful development of multiple‐stress‐tolerant, climate‐smart rice for southern and southeastern Asia

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