Marker-assisted pyramiding of opaque2 and novel opaque16 genes for further enrichment of lysine and tryptophan in sub-tropical maize

Sarika, Konsam; Hossain, Firoz; Muthusamy, Vignesh; Zunjare, Rajkumar U.; Baveja, Aanchal; Goswami, Rajat; Bhat, Jayant S.; Saha, Supradip; Gupta, H. S.

doi:10.1016/j.plantsci.2018.04.014

Cited by 69 publications

(40 citation statements)

References 26 publications

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“…The high RPG also led to the higher level of similarity of introgressed progenies with original version for the leaf, stem, tassel, plant and kernel types. Zunjare, Hossain, et al (), Hossain et al () and Sarika et al () also reported MABB‐derived introgressed progenies resembling their original parents for various morphological traits. Comprehensive DUS characterization also indicated the high recovery of RPG (Hossain et al, ).…”

Section: Discussionmentioning

confidence: 94%

“…Two generations of backcross coupled with SSR‐based background selection were followed for recovering the RPG to the extent of ~93%. Several research groups worldwide have successfully used SSRs for the recovery of >90% of RPG in maize (Gupta et al, ; Hossain et al, ; Liu et al, ; Muthusamy et al, ; Sarika et al, ; Zunjare, Hossain, et al, ). The high RPG also led to the higher level of similarity of introgressed progenies with original version for the leaf, stem, tassel, plant and kernel types.…”

Section: Discussionmentioning

confidence: 99%

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Marker‐assisted introgression of rare allele of β‐carotene hydroxylase (crtRB1) gene into elite quality protein maize inbred for combining high lysine, tryptophan and provitamin A in maize

et al. 2019

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Vitamin A deficiency in humans is a widespread health problem. Quality protein maize (QPM) is a popular food rich in lysine and tryptophan, but poor in provitamin A (proA). Here, we report the improvement of an elite QPM inbred, HKI1128Q for proA using marker-assisted introgression of crtRB1-favourable allele. HKI1128 was one of the parental lines of three popular hybrids in India and was converted to QPM in our earlier programme. Severe segregation distortion for crtRB1 was observed in BC 1 F 1 , BC 2 F 1 and BC 2 F 2 . Background selection by 100 SSRs revealed mean recovery of 91.07% recurrent parent genome varying from 88.78% to 93.88%. Across years, introgressed progenies possessed higher mean β-carotene (BC: 9.22 µg/g), β-cryptoxanthin (BCX: 3.05 µg/g) and provitamin A (proA: 10.75 µg/ g) compared to HKI1128Q (BC: 2.26 µg/g, BCX: 2.26 µg/g and proA: 3.38 µg/g).High concentration of essential amino acids, viz. lysine (mean: 0.303%) and tryptophan (0.080%) in endosperm, was also retained. Multi-year evaluation showed that introgressed progenies possessed similar grain yield (1,759-1,879 kg/ha) with HKI1128Q (1,778 kg/ha). Introgressed progenies with higher lysine, tryptophan and proA hold immense potential as donors and parents in developing biofortified hybrids. K E Y W O R D Sbiofortification, lysine, provitamin A, quality protein maize, tryptophan, β-carotene hydroxylase

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Marker‐assisted introgression of rare allele of β‐carotene hydroxylase (crtRB1) gene into elite quality protein maize inbred for combining high lysine, tryptophan and provitamin A in maize

et al. 2019

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“…Yang et al (2013) reported that average lysine in o16o16based BC 2 F 4 seeds was 0.352%; some of the segregants (o16o16) possessed comparable lysine and tryptophan usually observed in o2o2 genotypes, thereby suggesting that o16 can be used as replacement to o2 in the QPM breeding program. Sarika et al (2018a) reported that the seed endosperm of o16o16 was vitreous and phenotypically similar to wild type (O16O16). The mutant did not influence the degree of kernel opaqueness in o2o2 genetic background as opaqueness in o2o2/O16O16 and o2o2/o16o16 was similar.…”

Section: Quality Protein Maize Genetics and Breedingmentioning

confidence: 99%

“…Marker-assisted breeding has also been initiated in India to combine both o2 and o16 for developing QPM hybrids with enhanced levels of lysine and tryptophan (Sarika et al, 2018b;Chand et al, 2019). Sarika et al (2018b) introgressed o16 into the parental lines of four commercial QPM hybrids (HQPM-1, HQPM-4, HQPM-5, and HQPM-7) released in India, using MABB. Reconstituted hybrids showed an average enhancement of 49 and 60% in lysine and tryptophan over the original hybrids, with highest enhancement amounting 64 and 86%, respectively.…”

Section: Molecular Breeding For Developing Improved Quality Protein Mmentioning

confidence: 99%

Molecular Breeding for Nutritionally Enriched Maize: Status and Prospects

et al. 2020

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Maize is a major source of food security and economic development in sub-Saharan Africa (SSA), Latin America, and the Caribbean, and is among the top three cereal crops in Asia. Yet, maize is deficient in certain essential amino acids, vitamins, and minerals. Biofortified maize cultivars enriched with essential minerals and vitamins could be particularly impactful in rural areas with limited access to diversified diet, dietary supplements, and fortified foods. Significant progress has been made in developing, testing, and deploying maize cultivars biofortified with quality protein maize (QPM), provitamin A, and kernel zinc. In this review, we outline the status and prospects of developing nutritionally enriched maize by successfully harnessing conventional and molecular marker-assisted breeding, highlighting the need for intensification of efforts to create greater impacts on malnutrition in maize-consuming populations, especially in the low-and middle-income countries. Molecular marker-assisted selection methods are particularly useful for improving nutritional traits since conventional breeding methods are relatively constrained by the cost and throughput of nutritional trait phenotyping.

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“…When a gene shows variable degree of expression among individuals, it is called (Hossain et al 2008a). Regions where light does not pass through appears as black (thus opaque) and indicates soft region, while the region that permits light to pass through appears as orange and represents the hard region (Hossain et al 2007;Sarika et al 2018b). With experience, one can also visualize the varying degree of opaqueness just by looking at grains.…”

Section: Fig 23 Incomplete Penetrance For Embryo Abortion By Matl Amentioning

confidence: 99%

Genetics on a maize cob: A teaching tool for schools

Hossain¹,

Bhat²,

Mohapatra³

et al. 2019

IJGPB

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Genetics occupies central position in biology and directly connects with all its branches. In fact, ‘life’ and ‘non-life’ meet each other in viruses and genes. Technologies emanating from genetics have had immense impact in various areas such as healthcare, agriculture, environment etc. Recent developments in stem cell biology and gene editing technologies not only promise cures for several ailments which were hitherto unthinkable but also raise concerns of irreparable harm that can result from unbridled use of these technologies. Therefore, basic understanding of genetics is necessary for all sections of society. Hence, genetics is now introduced at the school level. However, at least in the Indian context, genetics teaching in classrooms relies largely on blackboard or chart-based explanations. Unlike physics, chemistry and to some extent basic botany and zoology where models or live/preserved specimens are available to demonstrate fundamental concepts under classroom conditions, there is a dearth of material resources for teaching genetics. Maize has a long history of basic and applied genetics where a large number of well-characterized mutant stocks are available. To make learning experience interesting and engaging, we have developed maize genetic resources for use under classroom conditions. In particular, maize cob with seeds segregating for various clearly identifiable phenotypes presents unique opportunity to demonstrate basic genetic principles including statistical and population genetic concepts. In this article we describe these resources and explain how they can be used to demonstrate different concepts.

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Marker-assisted pyramiding of opaque2 and novel opaque16 genes for further enrichment of lysine and tryptophan in sub-tropical maize

Cited by 69 publications

References 26 publications

Marker‐assisted introgression of rare allele of β‐carotene hydroxylase (crtRB1) gene into elite quality protein maize inbred for combining high lysine, tryptophan and provitamin A in maize

Marker‐assisted introgression of rare allele of β‐carotene hydroxylase (crtRB1) gene into elite quality protein maize inbred for combining high lysine, tryptophan and provitamin A in maize

Molecular Breeding for Nutritionally Enriched Maize: Status and Prospects

Genetics on a maize cob: A teaching tool for schools

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