Marker-assisted pyramiding of Thinopyrum-derived leaf rust resistance genes Lr19 and Lr24 in bread wheat variety HD2733

Singh, Mona; Mallick, Niharika; Chand, Suresh; Kumari, Preetesh; Sharma, Jai Bhagwan; Sivasamy, M.; Jayaprakash, P.; Prabhu, K. V.; Jha, Shailendra K.; Vinod,

doi:10.1007/s12041-017-0859-7

Cited by 24 publications

(12 citation statements)

References 16 publications

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“…However, during the present investigation, selections made in BC 4 F 6 and BC 4 F 7 generations resulted in successful recombination of stem rust and powdery mildew resistance. Marker assisted pyramiding of two or more disease resistance genes were reported forYr70/ Lr76 & Lr37/Yr17/Sr38 (Gautam et al 2020), QYr.nafu-2BL & QYr.nafu-3BS (Hu et al 2020, Sr25, SrWeb & Sr50 (Yadav et al 2015), Lr19, Sr26 & Yr10 (Mallick et al, 2015), Lr19 & Lr24 (Singh et al, 2017), Lr19 & Lr28 (Bhawar et al, 2011) Marker-Assisted Selection (MAS) improves the efficiency of selection because the environment does not affect the expression of molecular markers. These markers can also be detected at all stages of plant growth, whereas phenotypic markers/expression often can only be identified at specific growth stage.…”

Section: Resultsmentioning

confidence: 99%

Marker assisted stacking/pyramiding of stem rust, leaf rust and powdery mildew disease resistance genes (Sr2/Lr27/Yr30, Sr24/Lr24 and Sr36/Pm6) for durable resistance in wheat (Triticum aestivum L.)

Nisha

Vikas²

2020

EJPB

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Wheat being a staple food crop is essential for food security. Rust diseases like stem and leaf rust continues to be a serious threat to wheat production at national and international level. In recent years, powdery mildew disease is emerging as a threat to wheat production due to the climate change and intensive crop cultivation practices. Development of resistant cultivar is the most economical, reliable and sustainable way to control rusts and powdery mildew diseases. Stem rust, leaf rust and powdery mildew resistance genes viz., Sr2/Lr27/Yr30, Sr24/Lr24 and Sr36/ Pm6 were pyramided using marker assisted backcross breeding approach. Based on phenotypic observations, five populations were selected at BC 4 F 6 with respect to yield traits, leaf and stem rust and powdery mildew reactions. These populations were subjected to molecular marker analyses. Out of the five populations, Sr2+ were present in all the populations, while Sr24/Lr24 and Sr36/Pm6 was present in the fourth and fifth population. The identified population carrying Sr24/Lr24, Sr36/Pm6 and Sr2+ genes were stabilized at BC 4 F 7 generation. The use of gene stacks/pyramids of major (Sr24/Lr24 and Sr36/Pm6) and minor gene (Sr2+) that confer resistance to most of the pathotypes of stem rust, leaf rust and powdery mildew could impart durability and sustainability to the cultivars than single gene deployment.

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Section: Resultsmentioning

confidence: 99%

Marker assisted stacking/pyramiding of stem rust, leaf rust and powdery mildew disease resistance genes (Sr2/Lr27/Yr30, Sr24/Lr24 and Sr36/Pm6) for durable resistance in wheat (Triticum aestivum L.)

Nisha

Vikas²

2020

EJPB

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“…Once well-established, IWG weed competitiveness could be very substantial over its lifetime due to permanent soil cover, light interception, and nutrient use (Dick et al, 2018). Also, the Thinopyrum genus has already been shown responsible for better tolerance or resistance to important plant pathogens, such as fusarium head blight (Li et al, 2017;Mo et al, 2017;Singh et al, 2017). Diversifying perennial grain crops with intercropping or the implementation of insectary strips could further limit the development and spread of crop pests, and be part of an efficient strategy of pest control.…”

Section: 3control and Pest Managementmentioning

confidence: 99%

Integrating multipurpose perennial grains crops in Western European farming systems

Duchêne

Celette

Ryan

et al. 2019

Agriculture, Ecosystems & Environment

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Western European agriculture is largely defined by the high level of productivity of its cereal grain production. Such productivity is largely a result of farm specialization and intensification. This approach however has led to environmental problems and farm sensitivity to climatic and economic hazards. Recently, perennial grains have been promoted as a potential alternative, particularly with intermediate wheatgrass (Thinopyrum intermedium). Perennial grains bring new perspectives and innovation, and can contribute to both system diversification and environmental performance. Above all, the value of yearround ground cover and root activity, as well as the ability to harvest both grain and forage production, offers a large range of interest and potential application for such a grain crop. Realization of the potential benefits from perennial grains depends on the development of suitable seed material and the identification of tangible economic and environmental benefits coming from the integration of perennial grains into crop rotations. Although more work is needed, perennial grains are compatible with the current European interests and policies for food security, soil health, water quality, and farmer interest in innovative practices and sustainable cropping systems.

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“…Major gene pyramiding can reduce the possibility of major genes’ breakdown by combining multiple major genes for more durable resistance to multiple pathogen races into a single line. Pyramiding is implemented through marker-assisted selection (MAS) and has been an effective method for various crops (Wang et al, 2001; Pietrusińska et al, 2011; Bai et al, 2012; Jiang et al, 2012; Liu et al, 2016b; Singh et al, 2017; Wang et al, 2017). The successful implementation of major genes relies on identifying useful sources of the genes, finding linked markers, confirming the effect in different genetic backgrounds, and finally deploying said major genes (Bernardo, 2008).…”

Section: Introductionmentioning

confidence: 99%

Breeding with Major and Minor Genes: Genomic Selection for Quantitative Disease Resistance

Merrick

Burke

Chen

et al. 2021

Preprint

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Most disease resistance in plants is quantitative, with both major and minor genes controlling resistance. This research aimed to optimize genomic selection (GS) models for use in breeding programs needing to select both major and minor genes for resistance. In this experiment, stripe rust (Puccinia striiformis Westend. f. sp. tritici Erikss.) of wheat (Triticum aestivum L.) was used as a model for quantitative disease resistance. The quantitative nature of stripe rust is usually phenotyped with two disease traits, infection type and disease severity. We compared two types of training populations composed of 2,630 breeding lines phenotyped in single plot trials from four years (2016-2020) and 475 diversity panel lines from four years (2013-2016), both across two locations. We also compared the accuracy of models with four different major gene markers and genome-wide association (GWAS) markers as fixed effects. The prediction models used 31,975 markers replicated 50 times using 5-fold cross-validation. We then compared the GS models with marker-assisted selection to compare the prediction accuracy of the markers alone and in combination. The GS models had higher accuracies than marker-assisted selection and reached an accuracy of 0.72 for disease severity. The major gene and GWAS markers had only a small to zero increase in prediction accuracy over the base GS model, with the highest accuracy increase of 0.03 for major markers and 0.06 for GWAS markers. There was a statistical increase in accuracy by using the disease severity trait, the breeding lines, population type, and by combing years. There was also a statistical increase in accuracy using major markers within the validation sets as the mean accuracy decreased. The inclusion of fixed effects in low prediction scenarios increased accuracy up to 0.06 for GS models using significant GWAS markers. Our results indicate that GS can accurately predict quantitative disease resistance in the presence of major and minor genes.

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Marker-assisted pyramiding of Thinopyrum-derived leaf rust resistance genes Lr19 and Lr24 in bread wheat variety HD2733

Cited by 24 publications

References 16 publications

Marker assisted stacking/pyramiding of stem rust, leaf rust and powdery mildew disease resistance genes (Sr2/Lr27/Yr30, Sr24/Lr24 and Sr36/Pm6) for durable resistance in wheat (Triticum aestivum L.)

Marker assisted stacking/pyramiding of stem rust, leaf rust and powdery mildew disease resistance genes (Sr2/Lr27/Yr30, Sr24/Lr24 and Sr36/Pm6) for durable resistance in wheat (Triticum aestivum L.)

Integrating multipurpose perennial grains crops in Western European farming systems

Breeding with Major and Minor Genes: Genomic Selection for Quantitative Disease Resistance

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