Marker-assisted pyramiding of two cereal cyst nematode resistance genes from Aegilops variabilis in wheat

Barloy, Dominique; Lemoine, Jocelyne; Abélard, Paulette; Tanguy, Anne-Marie; Rivoal, Roger; Jahier, Joseph

doi:10.1007/s11032-006-9070-x

Cited by 99 publications

(61 citation statements)

References 35 publications

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“…So far, 9 resistance genes have been identified as controlling CCNs in bread wheat: Cre1 and Cre8 from Triticum aestivum; Cre2, Cre5, and Cre6 from Aegilops ventricosa; Cre3 and Cre4 from Triticum tauschii; Cre7 from Aegilops triuncialis; and CreR from Secale cereale (Barloy et al, 2007). In a recent study conducted by İmren et al (2012a) to identify genetic resistance to the cereal cyst nematodes H. avenae, H. filipjevi, and H. latipons, 6 Cre genes were used in some international bread wheat germplasms.…”

Section: Discussionmentioning

confidence: 99%

Resistance of winter wheat to Heterodera filipjevi in Turkey

Dababat¹,

Erginbaş-Orakci²,

Toktay³

et al. 2014

Turk J Agric For

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Section: Discussionmentioning

confidence: 99%

Resistance of winter wheat to Heterodera filipjevi in Turkey

Dababat¹,

Erginbaş-Orakci²,

Toktay³

et al. 2014

Turk J Agric For

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“…Markerassisted selection (MAS) has a great potential for increasing the efficiency of the breeding process by increasing the number of traits that can be selected in one population and by the precision with which genotypes can be selected. This method has been applied in the improvement of some crops and has been shown to be useful (Benchimol et al, 2005;Zhang et al, 2006;Barloy et al, 2007;Park et al, 2007;Nocente et al, 2007). Extensive research has been performed to identify markers associated with the desirable traits in crops.…”

Section: Introductionmentioning

confidence: 99%

Association of AFLP and SCAR markers with common leafspot resistance in autotetraploid alfalfa (Medicago sativa)

Wang¹,

Bi²,

Yuan³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. To identify amplified fragment length polymorphism (AFLP) markers associated with resistance or susceptibility of alfalfa to common leafspot (CLS) caused by the fungus Pseudopeziza medicaginis (Dermateaceae), bulked segregant analysis was conducted based on an F 1(M × M) population of 93 plants and a BC 1 S population of 91 plants. Three AFLP markers, ACTCAA R206 , TAGCAC R185 , and GGACTA S264 , were found to be associated with CLS resistance or susceptibility. All three markers were found at significantly different frequencies (71.9, 80.3 and 91.8%) compared to resistant or susceptible plants in the original population. Subsequently, these three AFLP markers were converted into three SCAR markers, ACTCAA R136 , TAGCAC R128 and GGACTA S254 , which are easier to employ in breeding programs. The three SCAR markers were used in a randomly selected population with 50% resistance; the probability of finding one resistant plant was increased to 67.3, 66.7 and 90.0% with markers ACTCAA R136 , TAGCAC R128 and GGACTA S254 , independently. If two of the SCAR markers were used simultaneously, the probability would be higher than 89%. The three SCAR markers identified in this study would be applicable for selection for CLS resistance in alfalfa breeding programs. Moreover, the genetic analysis indicated that CLS resistance in alfalfa is conferred by a single dominant gene.

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“…Some of these genes have been introgressed into wheat backgrounds (Barloy et al, 2007). Among the known Cre genes, only Cre3 was isolated through map-based cloning (Lagudah et al, 1997), and only Cre3 and CreZ have had their complete open reading frame (ORF) sequences registered in GenBank.…”

Section: Introductionmentioning

confidence: 99%

“…variabilis No. 1 (2n = 28,UUS v S v ) is resistant to root-knot nematode, Meloidogyne naasi and French pathotypes of CCN (Barloy et al, 2007). Ae.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a candidate gene for resistance to cereal cyst nematode from Aegilops variabilis in China

Xu¹,

Li²,

Long³

et al. 2012

Afr. J. Biotechnol.

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Cereal cyst nematode (CCN) (Heterodera avenae Woll.) is one of the most economically damaging endoparasite pests of wheat worldwide. We isolated and characterized a novel cereal CCN resistance candidate gene, CreV8, from Aegilops variabilis (2n = 28, UUS v S v ). The gene was 3,568 bp long and showed high nucleotide similarity with the known nematode resistance genes Cre3, CreZ, and go35. CreV8 consisted of two exons, one intron and a complete ORF of 2,763 bp. The deduced peptide sequence consisted of 920 amino acid residues and shared 97.83% amino acid identity with Cre3. The sequence harbored a signal peptide domain, a nucleotide-binding ARC (NB-ARC) domain, and a leucine-rich repeat (LRR) domain, all of which are typical characteristics of resistance genes. We proposed the resistance mechanism of CreV8 based on functional analysis and predictions from its conserved domains and tertiary structure. Real-time polymerase chain reaction (PCR) showed that CreV8 was expressed in CCN-inoculated roots of resistant plants, and its expression levels were 2.80 and 27.45 times higher than that in the untreated control at 3 and 11 days post-inoculation, respectively. These results indicate that CreV8 belongs to the nucleotide binding site (NBS)-LRR resistance gene family and that it is a candidate CCN-resistance gene.

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Marker-assisted pyramiding of two cereal cyst nematode resistance genes from Aegilops variabilis in wheat

Cited by 99 publications

References 35 publications

Resistance of winter wheat to Heterodera filipjevi in Turkey

Resistance of winter wheat to Heterodera filipjevi in Turkey

Association of AFLP and SCAR markers with common leafspot resistance in autotetraploid alfalfa (Medicago sativa)

Isolation and characterization of a candidate gene for resistance to cereal cyst nematode from Aegilops variabilis in China

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