Marker Assisted Selection for Blackspot Resistance in Roses

Debener, T.; Malek, B. von; Mattiesch, L.; Kaufmann, H.

doi:10.17660/actahortic.2001.547.41

Cited by 11 publications

(13 citation statements)

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“…MAS enables accurate selection regardless of environmental factors. MAS has been applied in breeding for resistance to black spot 6 , but as far as I know, no development of a commercial rose cultivar through MAS has been reported. Therefore, I think that the production of 'Karen Rouge' by MAS represents an important advance in the breeding of ornamentals.…”

Section: Resultsmentioning

confidence: 99%

Application of DNA Markers for Breeding Carnations Resistant to Bacterial Wilt

Yagi

2013

JARQ

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The carnation (Dianthus caryophyllus L.) is one of the major floricultural crops, not only in Japan but also worldwide. However, carnation production in Japan faces severe problems, such as the surge in carnations imported from overseas, e.g. Colombia and China, the slump in prices, and aging of growers. In 2010, imports accounted for 46.2% of the carnation market in Japan 30 , the highest proportion of any ornamental plant. One of a range of strategies needed to break out of the present situation is "Breeding revolutionary new cultivars." Japanese growers must produce fresher and better quality carnations to compete with imports. Growers cannot compete with imports on quality using the same cultivars used by exporting countries because the climatic conditions, including day length and temperature, are more favorable for carnation production in exporting countries than in Japan. It is desirable to develop an original Japanese cultivar suitable for the Japanese climate and appealing to Japanese consumers. To achieve this, revolutionary techniques to produce new cultivars within a short period are needed.The extensive study of plant genomes has sparked a revolution in molecular technique, while molecular markers have facilitated research into genetic variation at the DNA level. Marker-assisted selection (MAS), a method using DNA markers closely linked to relevant traits, holds great promise as a means of boosting the efficiency of the breeding line selection process. MAS enables accurate selection regardless of environmental factors and is possible even at the seedling stage, provided DNA can be extracted. MAS has been used in many crop breeding programs, and many commercial cultivars have been developed 40 . In ornamentals, expectations for the practical application of molecular markers have increased 4, 9, 32 . In recent years, floricultural research has focused on constructing genetic linkage maps and mapping genes for disease resistance, flower color, and other traits. This report reviews recent progress in the development of markers in carnation breeding for resistance to carnation bacterial wilt (CBW) by the NARO Institute of Floricultural Science (NIFS, Ibaraki, Japan). AbstractCarnation bacterial wilt (CBW), caused by Burkholderia caryophylli, is one of the most damaging diseases affecting carnations in Japan. In this study, carnation breeding was conducted using CBW resistance derived from Dianthus capitatus ssp. andrzejowskianus. To map the genetic loci involved in resistance to CBW and develop the linked markers, the first molecular linkage map for carnation was constructed by using 134 progeny derived from a cross between 'Carnation Nou No. 1' (an interspecific hybrid of carnation and D. capitatus) and 'Pretty Favvare', a susceptible cultivar. The map consisted of 146 DNA markers and covered 16 linkage groups. QTL analysis identified a QTL with a significant effect and two QTLs with small effects. Evaluation of disease incidence in relation to the presence of the STS-WG44 marker, which is linked to a ...

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Section: Resultsmentioning

confidence: 99%

Application of DNA Markers for Breeding Carnations Resistant to Bacterial Wilt

Yagi

2013

JARQ

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“…The roses used in the present study are a part of the genotype collection at the Department of Molecular Plant Breeding, Institute for Plant Genetics, Leibniz Universität Hannover (Germany). The genotypes 88/124‐46, 91/100‐5, 95/3‐23 and 99/20‐35 and the population 07/57 have been described previously (Debener et al ., ; Schulz et al ., ). All rose genotypes were cultivated under semi‐controlled conditions in the glasshouse at Leibniz Universität Hannover.…”

Section: Methodsmentioning

confidence: 97%

“…Using amplified fragment length polymorphism (AFLP) markers, the resistant genotype 95/3-23 with a small donor fraction was selected and backcrossed as a male parent to 'Heckenzauber' leading to the population 99/20 (Debener et al, 2003). Inoculation experiments provided evidence of the presence of a single dominant resistance locus in the duplex configuration in the hybrid 91/100-5.…”

Section: Introductionmentioning

confidence: 99%

The TNL gene Rdr1 confers broad‐spectrum resistance to Diplocarpon rosae

Menz

Straube

Linde

et al. 2017

Molecular Plant Pathology

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Black spot disease, which is caused by the ascomycete Diplocarpon rosae, is the most severe disease in field-grown roses in temperate regions and has been distributed worldwide, probably together with commercial cultivars. Here, we present data indicating that muRdr1A is the active Rdr1 gene, a single-dominant TIR-NBS-LRR (Toll/interleukin-1 receptor-nucleotide binding site-leucine rich repeat) (TNL)-type resistance gene against black spot disease, which acts against a broad range of pathogenic isolates independent of the genetic background of the host genotype. Molecular analyses revealed that, compared with the original donor genotype, the multiple integrations that are found in the primary transgenic clone segregate into different integration patterns in its sexual progeny and do not show any sign of overexpression. Rdr1 provides resistance to 13 different single-spore isolates belonging to six different races and broad field mixtures of conidia; thus far, Rdr1 is only overcome by two races. The expression of muRdr1A, the active Rdr1 gene, leads to interaction patterns that are identical in the transgenic clones and the non-transgenic original donor genotype. This finding indicates that the interacting avirulence (Avr) factor on the pathogen side must be widespread among the pathogen populations and may have a central function in the rose-black spot interaction. Therefore, the Rdr1 gene, pyramided with only a few other R genes by sexual crosses, might be useful for breeding roses that are resistant to black spot because the spread of new pathogenic races of the fungus appears to be slow.

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“…Early steps in achieving this goal would be to identify roses with strong resistance to D. rosae and to identify those roses in which resistance is determined by dominant major genes. Marker‐assisted selection for Rdr1 in segregating populations is now possible (Debener et al ., 2003; Kaufmann et al ., 2003) and markers of other resistance genes will be needed if their coinheritance is to be tracked through the generations in breeding programmes.…”

Section: Introductionmentioning

confidence: 99%

Interactions of four pathotypes of Diplocarpon rosae with species and hybrids of Rosa

Allum¹,

Bringloe²,

Roberts³

2010

Plant Pathology

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The interactions of four pathotypes of Diplocarpon rosae with 34 species and hybrids of Rosa were compared in an ongoing search for criteria of potential relevance to rose breeding. There were greater similarities in the resistance-susceptibility interactions of these roses to the pathotypes DA1, CW1 and ZM1, than to a fourth pathotype, DA2. Among the species roses, only those of the section Cinnamomeae were susceptible to DA2 and this susceptibility was transmitted in several cases to hybrid progeny. CW1 had the greatest pathogenicity of the four pathotypes. In a cross between R. rugosa cv. Scabrosa, which was resistant and R. rugosa f. alba, which was susceptible to all pathotypes, the progeny were tested for resistance to pathotypes DA1, CW1 and ZM1. Each of the 20 progeny was susceptible to all three pathotypes. This shows that, unlike the well characterized Rdr1 gene for resistance to D. rosae, the resistance of R. rugosa cv. Scabrosa is not determined by a dominant major gene. The diploid hybrid, R. rugosa cv. Martin Frobisher · cv. Mistress Quickly, was resistant to all four pathotypes, but an induced tetraploid of this hybrid was susceptible to all pathotypes. The relevance of these findings to the breeding of roses for resistance to black spot disease is discussed.

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Marker Assisted Selection for Blackspot Resistance in Roses

Cited by 11 publications

References 0 publications

Application of DNA Markers for Breeding Carnations Resistant to Bacterial Wilt

Application of DNA Markers for Breeding Carnations Resistant to Bacterial Wilt

The TNL gene Rdr1 confers broad‐spectrum resistance to Diplocarpon rosae

Interactions of four pathotypes of Diplocarpon rosae with species and hybrids of Rosa

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