Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

Abstract

Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F 3 progenies derived from the cross of Rathu Heenati and MR276 rice cultivars to investigate the association with BPH resistance against biotypes 2 and 3. For statistical analysis, parents and their offspring were grouped into 2 phenotypic classes based on their levels of BPH resistance. Chi-square analysis demonstrated a good fit to a ratio of 3:1 for the segregation of resistance and susceptibility. Association of SSR markers with phenotypic traits in F 3 progenies were identified. Six SSR markers (RM401, RM5953, RM217, RM210, RM242, and RM1103) were significantly associated with BPH resistance to biotypes 2 and 3 of BPH. Out of these 6 markers, RM401, RM5953, and RM217 accounted for about 17% of total phenotypic variation and RM210, RM242, and RM1103 accounted for 20% of total phenotypic variation against biotypes 2 and 3, respectively. Therefore, the above 6 markers were confirmed for MAS in rice breeding programs to develop BPH-resistant rice cultivars.

The effect of gamma ray re-irradiation on genetic variations in black rice based on RAPD and Bph gene resistance location based on SSR markers

“…According to Chaerani, et al (2014) [16], the size of the DNA bands formed from primers RM 124 and RM 17 are 225-328 bp and 178-208 bp respectively. Meanwhile, the size of the DNA band formed from primer RM 5953 and according to Shabanimofrad, et al (2015) [37] is 129 bp. The three samples that were successfully sequenced were samples from primer RM5953 amplicon.…”

Biotechnological Approaches for Host Plant Resistance to Insect Pests

“… Liu et al (2016) investigated that the pyramiding of two brown plant hopper resistance genes Bph3 and Bph27 (t), into elite rice cultivars through marker-assisted pyramiding showed significantly enhanced resistance against BPH and reduction in the yield loss caused by BPH. Shabanimofred et al (2015) developed rice cultivars through marker-assisted selection (MAS) that provided resistance in rice against biotypes 2 and 3 of brown planthopper (BPH). Sharma et al (2004) used marker-assisted pyramiding to successfully construct the Bph1 and Bph2 resistance genes on rice chromosome 12 to provide resistance against rice BPH.…”

Recent Strategies for Detection and Improvement of Brown Planthopper Resistance Genes in Rice: A Review

“…Application of molecular markers facilitates and hastens the desired gene introgression processes due to the fact that it saves time on mass or phenotypic selection, reduces the costs involved and accords more reliability to the selection result, which is free from the effect of environmental actors [ 66 , 67 ]. Several molecular markers have been adopted for the detection and introgression of genes that accord BPH resistance in rice, and they are sequence-tagged sites (STSs) [ 38 , 62 ], simple sequence repeats (SSRs) [ 32 , 64 , 68 , 69 , 70 , 71 , 72 ], amplified fragment length polymorphisms (AFLPs) [ 63 , 73 ], single nucleotide polymorphisms (SNPs) [ 13 , 31 , 74 ], rapid amplified polymorphisms DNAs (RAPDs) [ 62 , 75 ], rapid fragment length polymorphisms (RFLPs) [ 63 , 73 , 76 , 77 ], and insertion deletions (InDels) [ 28 , 33 , 34 , 78 , 79 ].…”