Marker‐free transgenic (MFT) near‐isogenic introgression lines (NIILs) of ‘golden’ indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue

Abstract

SummaryWe have developed near-isogenic introgression lines (NIILs) of an elite indica rice cultivar

Enhanced salt stress tolerance of rice plants expressing a vacuolar H+‐ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Löisel

“…The products of the primary, secondary and tertiary reactions were analysed on a 1.5% agarose gel. Fragments exhibiting a difference in size consistent with nested gene‐specific primer positions were cloned using pGEMT‐easy vector (Promega, Madison, WI) and sequenced as described earlier (Baisakh et al. , 2006b).…”

Nutritionally Enhanced Staple Food Crops

“…The proof of concept has been demonstrated that genes psy and crtI or psy, crtI and lycopene b-cyclase under endosperm-specific promoters have been effective for elevating b-carotene concentration in transgenic rice, with more b-carotene in polished grains. More importantly, the incorporation of genes for carotenogenesis in seeds by transgenesis or by introgression did not change any significant agronomic characteristics in rice plants (Paine et al 2005;Baisakh et al 2006;Datta et al 2006Datta et al , 2007.…”

Golden rice: introgression, breeding, and field evaluation

“…The differences between the introgression lines in a subcluster were negligible and would be further minimized in the next BC generation. This suggested that the introgression of the two genes (psy and crtI) capable of installing the entire carotenogenic pathway only resulted in minor genome changes in IR64 (Baisakh et al 2006). Introgressed IR64 from original japonica rice (T309) also showed lower carotenoid levels (Baisakh et al 2006; Table 1).…”

“…This suggested that the introgression of the two genes (psy and crtI) capable of installing the entire carotenogenic pathway only resulted in minor genome changes in IR64 (Baisakh et al 2006). Introgressed IR64 from original japonica rice (T309) also showed lower carotenoid levels (Baisakh et al 2006; Table 1). Efforts have been made to select a transgenic event with a simple and low-copy transgene for a Mendelian 3:1 segregation pattern and eventually to develop homozygous lines with acceptable agronomic performance.…”

“…Homozygous lines (DH line, KDHR3-11 from Z4b containing 1.1 lg/g carotenoids) were preferred as the best materials available at that time for cross breeding to transfer the genes/traits into the desirable cultivars. Eventually, introgressed IR64 lines were developed using the selected DH lines described in details elsewhere (Baisakh et al 2006).…”