Marker Profiling of Botanicals Used for Hepatoprotection in Indian System of Medicine

Abstract

JQuality control of botanicals, including phytomedicines and dietary supplements, is a basic requirement to ensure their safety and effectiveness. Qualitative and quantitative analyses of marker components is a potentially cost-efficient aspect of quality control as any change in quality of the marker compound may also represent the quality of overall product, which would also directly aflect the other phytoconstituents of the product.Our studies attempted to develop the marker profile of Andrographis paniculata, Berberis aristata, and Phyllanthus amarus, which are used as hepatoprotectives in the Indian system of medicine. The plant extracts were subjected for high-peformance thin-layer chromatographic analysis along with the respective standard markers. Andrographolide appeared at retardation factor & 0.7 in a methanolic extract of A. paniculata with a chlorofonn:methanol (70: 10) solvent system. Berberine appeared at R,3.6 in apetroleum ether extract ofB. aristata with an n-propanol:fonnic acid:water (90: 1 :9) solvent system. Phyllanthin and hypophyllanthin appeared at Rf 0.3 and 0.4, respectively, in a methanolic extract of I? amarus with a hexane:ethyl acetate (2~1) solvent system. Densitometric scanning produced characteristic chromatographs. Such characteristic chromatographs can be used as standardfingerprint to develop a quality control protocol for plant materials.

Phyllanthus amarus: Ethnomedicinal uses, phytochemistry and pharmacology: A review

“…Methanolic extract of P. amarus and standard phyllanthin and hypophyllanthin were developed in the chromatographic conditions. They showed the presence of standard phyllanthin and hypophyllanthin at R f 0.3 and 0.4 respectively, by densitometric scanning at 254 nm (Mukherjee et al, 2006). Separation of phyllanthin and hypophyllanthin was carried out on silica gel 60F 254 layers eluted with hexane:acetone:ethyl acetate (74:12:8), and the analytes were visualised through colour development with vanillin in concentrated sulfuric acid and ethanol.…”

Immunomodulatory potential of rhizome and seed extracts of Nelumbo nucifera Gaertn.

“…Many plants have been extensively used as 'Rasayana' drugs in 'Ayurveda' for the management of neurodegenerative diseases, as well as rejuvenators, immunomodulators, aphrodisiac and nutritional supplements (Govindarajan et al, 2005;Mukherjee et al, 2006).…”

Estimation of biomarkers berberine and gallic acid in polyherbal formulation punarnavashtak kwath and its clinical study for hepatoprotective potential

“…The repeatability of the method was checked by repeated scanning of the same spot of berberine (32 ng) and gallic acid (400 ng) seven times and was expressed as coefficient of variance (% CV). Variability of the method was studied by analysing aliquots of standard solution of berberine (16,40, 64 ng/ spot) and gallic acid (400, 800, 1200 ng/spot) on the same day (intraday precision) and on different days (interday precision) and the results were expressed as % CV.…”

“…dioica was reported as a hepatoprotective agent in ferrous sulphate (FeSO 4 ) intoxicated rats. [16] Polyherbal formulations have synergistic potentiative agonistic/antagonistic pharmacological agents within themselves, which work together in a dynamic way to produce therapeutic efficacy with minimum side effects.…”