Marker succession during the development of keratinocytes from cultured human embryonic stem cells

Abstract

Human embryonic stem cells injected into scid mice produce nodules containing differentiated somatic tissues. From the trypsinized cells of such a nodule, we have recovered keratinocytes that can be grown in cell culture. The method of recovery is sensitive enough to detect small numbers of keratinocytes formed in the nodule, but for purposes of analysis, it is preferable to study the development of the entire keratinocyte lineage in culture. The principle of our analysis is the successive appearance of markers, including transcription factors with considerable specificity for the keratinocyte (p63 and basonuclin) and differentiation markers characteristic of its final state (keratin 14 and involucrin). We have determined the order of marker succession during the time-and migration-dependent development of keratinocytes from single embryoid bodies in cell culture. Of the markers we have examined, p63 was the earliest to appear in the keratinocyte lineage. The successive accumulation of later markers provides increasing certainty of emergence of the definitive keratinocyte.

TAp63α indirectly regulates a cutaneous HPV promoter through complex formation with Jun family members

“…The continued expression of TAp63 is, however, required for the maintenance of the proliferative potential of the basal cells . The appearance of p63 as a marker of the keratinocyte lineage in human cells appears to be in line with the situation in mice (Green et al, 2003). The expression pattern of the Jun proteins in skin, as well as reports on in vitro studies, point to a delicate interplay between the subunits of the AP-1 complex, influenced by their individual binding and tissue-specificity (Angel and Szabowski, 2002;Mehic et al, 2005).…”

Niche regulation of corneal epithelial stem cells at the limbus

“…The earliest transcription factor expressed when embryonic SCs are destined to the keratinocyte lineage is p63 [111]. However, the first recognized ectodermal gene expression associated with eye development is PAX 6 [112], which is consistently turned on by the ocular surface epithelium postnatally [113].…”

Association of differential β‐catenin expression with Oct‐4 and Nanog in oral squamous cell carcinoma and their correlation with clinicopathological factors and prognosis

“…Involucrin expression is also dependent on the level of hypoxia in the tumor tissues [26]. During the in vitro differentiation of human embryonic stem cells to keratinocytes, the expression of Involucrin was found only during the initiation of keratinocyte differentiation [27].…”