Marker Validation in F2 Population of Rice (Oryza sativa L.) for  Water and Nitrogen Use Efficiency

Boranayaka, M. B.; Lokesha, R.; Diwan, J. R.; Patil, Rajendragouda

doi:10.20546/ijcmas.2018.701.155

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…However, only nine SSR markers involved in Saltol QTL are polymorphic, including seven SSR markers locate on chromosome 1 (RM493, RM562, RM10694, RM10720, RM10793, RM10852, and RM13197), and two markers RM201 and RM149 located on chromosome 8 and 9, respectively [8][9][10] (Figure 2; Supplementary Table S2). Other polymorphic markers were identified relating to plant height (RM202, RM206, RM219, RM229) [12], leaf diameter (RM508) [43], spikelet fertility (RM202, RM206) [12] spikelet number (RM229) [12], grain yield (RM219, RM432) [12], amylose content (RM219, RM432, RM508, RM587, RM589) [12], gel consistency (RM589) [12], blast resistance (RM1233, RM206, RM207, RM213) [12,43], brown planthopper resistance (RM206) [12], and water and nitrogen use efficiency (RM518) [45] (Supplementary Table S2). Other SSRs relevant to the Saltol QTL are not polymorphic and they could not be used to identify uniparentally controlled by MNU treatment (Supplementary Figure S3).…”

Section: Discussionmentioning

confidence: 99%

“…A total of fifty SSR markers which were reported previously to be involved in growth parameters, yield, pest resistance, and the Saltol QTL (salinity tolerant) [7][8][9][10]12,[31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] were selected to evaluate the differences among genotypes of the recurrent TBR1 and male KD18 cultivars (Supplementary Figure S3). These markers are distributed throughout 12 chromosomes of the rice genome (Supplementary Table S2).…”

Section: Genetic Analysismentioning

confidence: 99%

“…Besides these, markers relevant to other Saltol QTLs, such as plumule (shoot) fresh weight and root Na-K ratio, are RM 149 (chromosome 8) and RM 201 (chromosome 9), respectively [9,10]. Other polymorphic markers were identified relating to growth parameter, grain yield and quality, and pest resistance, as well as water and nitrogen use efficiency [12,41,43,45]. These polymorphic markers therefore were used to identify F 2 , M 2 and M 3 genotypes.…”

Section: Genetic Analysismentioning

confidence: 99%

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Uniparental Inheritance of Salinity Tolerance and Beneficial Phytochemicals in Rice

Huong

Anh

Dat³

et al. 2020

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Salinity stress is one of the most problematic constraints to significantly reduce rice productivity. The Saltol QTL (quantitative trait locus) has been known as one among many principal genes/QTLs responsible for salinity tolerance in rice. However, the introgression of the Saltol QTL from the donor (male) into the recipient (female) cultivars induces great recessions from the progeny generation, which results in heavy fieldwork and greater cost and time required for breeding. In this study, the F1 generation of the cross TBR1 (female cultivar, salinity tolerant) × KD18 (male cultivar, salinity susceptible) was preliminarily treated with N-methyl-N-nitrosourea (MNU) to induce the mutants M1. Results on physiological traits show that all the M2 (self-pollinated from M1) and M3 (self-pollinated from M2) individuals obtain salinity tolerant levels as the recurrent TBR1. Twelve SSR (simple sequence repeat) markers involved in the Saltol QTL (RM493, RM562, RM10694, RM10720, RM10793, RM10852, RM13197, RM201, RM149, RM508, RM587, and RM589) and other markers related to yield-contributing traits and disease resistance, as well as water and nitrogen use, have efficacy that is polymorphic. The phenotype and genotype analyses indicate that the salinity tolerant Saltol QTL, growth parameter, grain yield and quality, pest resistance, water and nitrogen use efficacy, and beneficial phytochemicals including antioxidants, momilactone A (MA) and momilactone B (MB) are uniparentally inherited from the recurrent (female) TBR1 cultivar and stabilized in the M2 and M3 generations. Further MNU applications should be examined to induce the uniparental inheritance of other salinity tolerant genes such as OsCPK17, OsRMC, OsNHX1, OsHKT1;5 to target rice cultivars. However, the mechanism of inducing this novel uniparental inheritance for salinity tolerance by MNU application needs elaboration.

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