Markers for Hematopoietic Stem Cells: Histories and Recent Achievements

Yokota, Takafumi; Oritani, Kenji; Butz, Stefan; Ewers, Stephan; Vestweber, Dietmar; Kanakura, Yuzuru

doi:10.5772/32381

Cited by 12 publications

(5 citation statements)

References 46 publications

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“…Studies with such sophisticated strategies have switched or enlarged the notion of homeostatic HSCs from classically defined long-term HSCs to long-lived progenitors [32,33]. That is, while conventional transplantation experiments demonstrated that a very small number of multipotent and self-renewing HSCs, of which phenotypes were determined as lineage makers⁻ c-kit High Sca1⁺ Flt3⁻ CD48⁻ CD150⁺ ESAM⁺ EPCR⁺ in mouse and lineage makers CD34⁺ CD38⁻ /Low ESAM⁺ CD90/Thy1⁺ CD45RA⁻ CD49f⁺ in human [34,35,36,37,38,39,40] which reconstitute the hematopoietic system, the contribution of those long-term HSCs to physiological hematopoiesis is minimal. Instead, short-term HSCs and multipotent progenitors primarily sustain adult hematopoiesis.…”

Section: Discussionmentioning

confidence: 99%

“…Our understanding of hematopoiesis traditionally represents the hierarchical tree diagram, in which HSCs differentiate to lineage-committed precursor cells in a stepwise manner through multipotent, oligopotent and bipotent stages (Figure 3A). Numerous studies have been conducted on this deep-seated notion and have identified the most immature HSCs as well as intermediate progenitors with surface markers [34,35,36,37,38,39,40]. Nevertheless, our hematopoietic system is considered to have evolved during the long process of mammalian evolution to the ideal, which cannot be confined to such a rigid hierarchical organization.…”

Section: Discussionmentioning

confidence: 99%

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“Hierarchy” and “Holacracy”; A Paradigm of the Hematopoietic System

Yokota

2019

Cells

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The mammalian hematopoietic system has long been viewed as a hierarchical paradigm in which a small number of hematopoietic stem cells (HSCs) are located at the apex. HSCs were traditionally thought to be homogeneous and quiescent in a homeostatic state. However, recent observations, through extramedullary hematopoiesis and clonal assays, have cast doubt on the validity of the conventional interpretation. A key issue is understanding the characteristics of HSCs from different viewpoints, including dynamic physics and social network theory. The aim of this literature review is to propose a new paradigm of our hematopoietic system, in which individual HSCs are actively involved.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“Hierarchy” and “Holacracy”; A Paradigm of the Hematopoietic System

Yokota

2019

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“…The status of maintenance of HSC at quiescence not only determines the lifelong self-renewing ability of HSC but also enhances their survivability by optimizing cell division [28] . In addition, up regulated Ang-1/Tie-2 signaling is believed to impart in the long-term repopulating ability of HSCs [55] . Flowcytometric studies showed [ Figure 4A and B] that following glioma induction Ang-1 expression BMHSCs of the ENU group in both the LDC and HDC compartments was remarkably (P < 0.001) elevated compared to low basal expression in normal BMHSCs in both the LDC and HDC.…”

Section: Modulation Of Tie-2/ang-1 Signaling Of Bone Marrow-derived Hmentioning

confidence: 99%

The novel-molecule T11TS facilitated arousal of glioma-mediated dormancy of bone-marrow hematopoietic stem-cells

Mondal¹,

Datta²,

Hazra³

et al. 2018

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Aim: T11TS, a potent anti-gliomagenic glycoprotein, stimulates both peripheral and intracranial immune response. The status of bone marrow hematopoietic stem cells (BMHSCs), the cradle of regeneration of all blood cells, during gliomagenic global immune devastations has not yet been investigated. Therefore, we aimed to delineate the effects of T11TS on immature and mature compartments of hematopoietic machinery. Methods: Flowcytometric analysis of cultured BMHSCs was evaluated for assesing the expression pattern of early hematopoietic stem cells (HSCs) markers such as CD34 + , Sca-1 + , c-kit + and also Angiopoietin-1 and Tie-2 both in normal, glioma, and in T11TS treated glioma-bearing animals. Immunofluresenece imaging and western blot analyses of BMHSCs were also carried out. Results: There was significant downregulation of HSCs-markers CD34 + , Sca-1 + , c-kit + in ethyl nitrosourea-induced gliomabearing animals followed by an increase in the expression level of Ang-1 and Tie-2 that determines the quiescence and self-renewability of stem cells. T11TS administration reversed the gliomagenic transformation of expression of the above mentioned markers. The results flowcytometric-analysis was also well corroborated with immunofluorescence imaging and western blot analysis. Conclusion: Collectively, the above experimental evidence hints towards gliomagenic maneuver of receptor expression of HSCs to derange the systemic immunity and T11TS mediated manipulation towards revival/rejuvenation of the same.

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“…Under physiological conditions, numbers of HSCs in bone marrow and in peripheral blood are very low. However, it is possible to purify reconstituting HSC from mouse bone marrow with very high effi ciency using different panels of specifi c surface markers (Yokota et al 2012 ). Nevertheless, there are signifi cant challenges with these methods for HSC isolation.…”

Section: Hematopoietic Stem Cellsmentioning

confidence: 99%