Markers for oxidative stress in the synovial fluid of Thoroughbred horses with carpal bone fracture

Abstract

Arthritis is thought to cause oxidative stress in synovial fluid in humans, but there have been few reports in horses. To evaluate oxidative stress in synovial fluid in horses, this study used 19 horses with unilateral fracture of the carpal joint bone. Synovial fluid was collected from the carpal joint on the fracture (arthritis group) and contralateral (control group) sides. Diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) were then measured, and the oxidative stress index (OSI) was calculated. d-ROMs and OSI of the arthritis group were significantly higher than the control group. BAP of the arthritis group was significantly lower than the control group. Thus, this study revealed that oxidative stress develops in the synovial fluid of horses during arthritis.

The Oxidative Stress Markers of Horses—the Comparison with Other Animals and the Influence of Exercise and Disease

“…d-ROM levels for horses having no disease but having experienced castration surgery or delivery were also distributed outside the normal range, indicating that it is not only disease that is reflected in d-ROM and BAP levels in serum. Although the synovial fluid was used in the study, d-ROMs and OSI levels in the synovial fluid of the arthritis group were significantly higher than the control group [12]. Because the study of dairy cattle also found higher concentrations of d-ROMs and lower concentrations of BAP in dairy cattle having left displacement of abomasum, it was suggested, when compared to the control group [25], that several diseases are thought to influence d-ROMs concertation of animals.…”

“…There have also been many studies in various animals using indexes related to oxidative stress and antioxidant activity such as 8-OHdG [7] and lipid peroxidation [8]. The aims of those studies were to cover a lot of ground, for example, animal welfare [9,10], produce of value-added animal product [11], efficient produce of animal product [7], and estimation of disease [12].…”