Markers of airway inflammation in pulmonary diseases assessed by induced sputum

Dragonieri, Silvano; Tongoussouva, O.; Zanini, Andrea; Imperatori, Andrea; Spanevello, Antonio

doi:10.4081/monaldi.2009.357

Cited by 15 publications

(15 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fluid phase of sputum has been less well characterized. It includes proteins, many of which have been linked to inflammatory airway diseases [ 10 ]. Several inflammatory markers, including eosinophil cationic protein, interleukins 8 and 13, and cysteinyl leukotrienes (CysLTs), have been detected in the fluid phase of sputum collected from asthmatics.…”

Section: Introductionmentioning

confidence: 99%

Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation

et al. 2015

View full text Add to dashboard Cite

BackgroundThe inflammatory processes in the upper and lower airways in allergic rhinitis and asthma are similar. Induced sputum and nasal lavage fluid provide a non-invasive way to examine proteins involved in airway inflammation in these conditions.ObjectivesWe conducted proteomic analyses of sputum and nasal lavage fluid samples to reveal differences in protein abundances and compositions between the asthma and rhinitis patients and to investigate potential underlying mechanisms.MethodsInduced sputum and nasal lavage fluid samples were collected from 172 subjects with 1) allergic rhinitis, 2) asthma combined with allergic rhinitis, 3) nonallergic rhinitis and 4) healthy controls. Proteome changes in 21 sputum samples were analysed with two-dimensional difference gel electrophoresis (2D-DIGE), and the found differentially regulated proteins identified with mass spectrometry. Immunological validation of identified proteins in the sputum and nasal lavage fluid samples was performed with Western blot and ELISA.ResultsAltogether 31 different proteins were identified in the sputum proteome analysis, most of these were found also in the nasal lavage fluid. Fatty acid binding protein 5 (FABP5) was up-regulated in the sputum of asthmatics. Immunological validation in the whole study population confirmed the higher abundance levels of FABP5 in asthmatic subjects in both the sputum and nasal lavage fluid samples. In addition, the vascular endothelial growth factor (VEGF) level was increased in the nasal lavage fluid of asthmatics and there were positive correlations between FABP5 and VEGF levels (r=0.660, p<0.001) and concentrations of FABP5 and cysteinyl leukotriene (CysLT) (r=0.535, p<0.001) in the nasal lavage fluid.ConclusionsFABP5 may contribute to the airway remodeling and inflammation in asthma by fine-tuning the levels of CysLTs, which induce VEGF production.

show abstract

Section: Introductionmentioning

confidence: 99%

Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Nebulized and inhaled saline increases sputum production in the lungs [ 4 ]. Induction has been reported to provide sputum samples of sufficient quality for analyses in more than 80% of asthma and COPD patients [ 5 – 8 ]. In patients with obstructive lung disease, ISS is usually performed in the steady state as it can induce bronchoconstriction [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of inflammatory markers in induced and spontaneous sputum in a cohort of COPD patients

et al. 2014

View full text Add to dashboard Cite

BackgroundSputum induction is a non-invasive method for obtaining measurements of inflammation in the airways. Whether spontaneously sampled sputum can be a valid surrogate is unknown. The aim of this study was to compare levels of six inflammatory markers in sputum pairs consisting of induced and spontaneous sputum sampled on the same consultation either in a stable state or during exacerbations of chronic obstructive pulmonary disease (COPD).Methods433 COPD patients aged 40–76, Global initiative for chronic Obstructive Lung Disease (GOLD) stage II-IV were enrolled in 2006/07 and followed every six months for three years. 356 patients were followed for potential exacerbations. Interleukin-6, interleukin-8, interleukin-18, interferon gamma-inducible protein-10, monokine induced by gamma interferon and tumor necrosis factor-alpha (IL-6, IL-8, IL-18, IP-10, MIG and TNF-α) were measured by bead based multiplex immunoassay in 60 paired sputum samples from 45 patients. Albumin was measured by enzyme immunoassay, for concentration correction. Culturing for bacterial growth was performed on 24 samples. Bland-Altman plots were used to assess agreement. The paired non-parametric Wilcoxon signed-rank test, the non-parametric Spearman’s rank correlation test and Kruskal-Wallis test were used for statistical analyses. For all analyses, a p-value < 0.05 was considered significant.ResultsAgreement between the two measurements was generally low for all six markers. TNF-α was significantly higher in spontaneous sputum at exacerbations (p = 0.002) and trending higher at the steady state (p = 0.06). Correlation coefficients between the levels of markers in induced and spontaneous sputum varied between 0.58 (IL-18) to 0.83 (IP-10). In spontaneous sputum IL-18 and MIG were higher in ex-smokers (p < 0.05). The levels of all markers were higher in GOLD stage III & IV except for IL-6 in spontaneous sputum and IL-18 in induced sputum, compared with GOLD stage II, although not statistically significant. In spontaneous sputum the levels of IL-6 were significantly higher if Haemophilus influenzae (HI) was not cultured.ConclusionWe observed a low agreement and significant differences in inflammatory markers between induced and spontaneous sputum, both at steady state and exacerbations. We recommend considering sampling method when reporting on inflammatory markers in sputum.

show abstract

“…The benefits of a simple, non-invasive sampling technique for asthma diagnosis are obvious and a great deal of work has focussed on the development of breath sampling, including exhaled nitric oxide 35 , measurement of volatile organic compounds 13,36 and the e-nose 37 .…”

Section: Discussionmentioning

confidence: 99%

Untargeted metabolic profiling of saliva by liquid chromatography-mass spectrometry for the identification of potential diagnostic biomarkers of asthma

et al. 2016

View full text Add to dashboard Cite

Colin (2016) Untargeted metabolic profiling of saliva by liquid chromatography-mass spectrometry for the identification of potential diagnostic biomarkers of asthma. Analytical Methods, 8 (27 A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. AbstractCurrent clinical tests employed to diagnose asthma are inaccurate and limited by their invasive nature. New metabolite profiling technologies offer an opportunity to improve asthma diagnosis using non-invasive sampling. A rapid analytical method for metabolite profiling of saliva is reported using ultra-high performance liquid chromatography combined with high resolution time-of-flight mass spectrometry (UHPLC-MS). The only sample pretreatment required was protein precipitation with acetonitrile. The method has been applied to a pilot study of saliva samples obtained by passive drool from well phenotyped patients with asthma and healthy controls. Stepwise data reduction and multivariate statistical analysis was performed on the complex dataset obtained from the UHPLC-MS analysis to identify potential metabolomic biomarkers of asthma in saliva. Ten discriminant features were identified that distinguished between moderate asthma and healthy control samples with an overall recognition ability of 80% during training of the model and 97% for model crossvalidation. The reported method demonstrates the potential for a non-invasive approach to the clinical diagnosis of asthma using mass spectrometry-based metabolic profiling of saliva.2

show abstract

Markers of airway inflammation in pulmonary diseases assessed by induced sputum

Cited by 15 publications

References 79 publications

Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation

Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation

Comparison of inflammatory markers in induced and spontaneous sputum in a cohort of COPD patients

Untargeted metabolic profiling of saliva by liquid chromatography-mass spectrometry for the identification of potential diagnostic biomarkers of asthma

Contact Info

Product

Resources

About