Markers of atopic dermatitis, allergic rhinitis and bronchial asthma in pediatric patients: correlation with filaggrin, eosinophil major basic protein and immunoglobulin E

Rasheed, Zafar; Zedan, Khaled; Saif, Ghada A. Bin; Salama, Ragaa H. M.; Ahmed, Ahmed A.; El-Moniem, Alaa Abd; Elkholy, Maha; Robaee, Ahmad A. Al; Alzolibani, Abdullateef A.

doi:10.1186/s12948-018-0102-y

Cited by 27 publications

(19 citation statements)

References 33 publications

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“…As a whole, the present findings clearly suggest the roles of multiple proteins in the pathogenesis of BA, AR and AD. Our results are fully supported by numerous studies performed in different disorders including allergic disorders (31)(32)(33)(34). In our previous studies we have also reported pathogenic effects of multiple proteins in patients with systemic lupus erythematosus (35)(36)(37).…”

Section: Discussionsupporting

confidence: 88%

Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitis

Rasheed

Salama

et al. 2018

IRDR

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Bronchial asthma (BA), atopic dermatitis (AD) and allergic rhinitis (AR) are common atopic disorders with complicated etiologies. The atopic march from early AD to BA, AR, or both later in life and the extensive comorbidity among them indicates, that these atopic disorders might share a common mechanism (1). Moreover, heritability of these atopic disorders is high, being 35-95% for BA, 71-84% for AD, 33-91% for AR and 34-68% for allergen-specific serum immunoglobulin E (IgE) levels (1,2). Filaggrin is now considered as a major predisposing gene for many atopic disorders, which result in a major paradigm in dermatology and allergy research (3). Many studies pointed out an association of the filaggrin gene with different atopic disorders. More specifically, loss-of-function mutations in the filaggrin gene have been reported to have an association with various atopic/allergic disorders (3). Batchelor et al., reported that there is a strong and consistent association between filaggrin mutations and development of AD (4), but Summary Bronchial asthma (BA), atopic dermatitis (AD), and allergic rhinitis (AR) are well known atopic disorders with complex etiologies. This study was undertaken to investigate the role of filaggrin, eosinophil major basic protein (MBP) and leukotriene B4 (LTB4) in patients with BA, AD, and AR. Sera from 1,246 patients with different atopic disorders and 410 normal healthy controls were collected and were evaluated for filaggrin, MBP and LTB4 by specific sandwich ELISAs, whereas immunoglobulin E (IgE) was used as a positive control for atopic patients. Serum analysis showed that filaggrin levels were remarkably high in patients with AD and in patients with multiple (mixed) atopic disorders (p < 0.001), whereas its levels in BA and AR patients were low but much higher than in normal human sera (p < 0.01). MBP levels were also high in AR, BA and mixed atopic patients, whereas AD patients showed no increase of MBP (p > 0.05). In contrast, LTB4 level was found to be significantly low in all tested atopic patients groups as compared to the levels of LTB4 present in normal human sera (p < 0.001). In conclusion, these findings support an association between filaggrin, MBP or LTB4 and atopic disorders. Our data strongly suggest that filaggrin, MBP or LTB4 might be useful in elucidating the mechanisms involved in the pathogenesis of these atopic disorders.

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Section: Discussionsupporting

confidence: 88%

Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitis

Rasheed

Salama

et al. 2018

IRDR

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“…Activated eosinophils in turn also secrete cytokines and chemokines, such as IL-16, IL-12, TGF-β1, and IL-13 and induce immunological reactions. Eosinophil granular proteins deposition and its degranulation observed in AD patients [166]. Topical calcineurin inhibitors like tacrolimus, pimecrolimus, immunomodulatory agents prednisolone, systemic steroid-sparing agents azathioprine, methotrexate, cyclosporine, mycophenolate are available treatments for AD [167,168].…”

Section: Atopic Dermatitis (Ad) or Eczemamentioning

confidence: 99%

Synergy of Interleukin (IL)-5 and IL-18 in eosinophil mediated pathogenesis of allergic diseases

Kandikattu

Venkateshaiah

Mishra

2019

Cytokine & Growth Factor Reviews

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Eosinophils are circulating granulocytes that have pleiotropic effects in response to inflammatory signals in the body. In response to allergens or pathogens, exposure eosinophils are recruited in various organs that execute pathological immune responses. IL-5 plays a key role in the differentiation, development, and survival of eosinophils. Eosinophils are involved in a variety of allergic diseases including asthma, dermatitis and various gastrointestinal disorders (EGID). IL-5 signal transduction involves JAK-STAT-p38MAPK-NFkB activation and executes extracellular matrix remodeling, EMT transition and immune responses in allergic diseases. IL-18 is a classical cytokine also involved in immune responses and has a critical role in inflammasome pathway. We recently identified the IL-18 role in the generation, transformation, and maturation of (CD101 + CD274 +) pathogenic eosinophils. In, addition, several other cytokines like IL-2, IL-4, IL-13, IL-21, and IL-33 also contribute in advancing eosinophils associated immune responses in innate and adaptive immunity. This review discusses with a major focus (1) Eosinophils and its constituents, (2) Role of IL5 and IL18 in eosinophils development, transformation, maturation, signal transduction of IL5 and IL18, (3) the role of eosinophils in allergic disorders and (4) the role of several other associated cytokines in promoting eosinophils mediated allergic diseases.

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“…It has been reported that granuloma eosinophils are highly activated and produce the majority of Th2 cytokines in granulomatous inflammation, which may be important determiners of immunopathology in murine schistosomiasis caused by S. mansoni (6). The eosinophil granulocytes contain four major proteins, i.e., eosinophil cationnic protein [ECP; (7)], eosinophil peroxidase [EPO; (8)], eosinophil protein X (EPX)/eosinophil-derived neurotoxin (9), and major basic protein [MBP; (10)]. Eotaxin is an essential regulator of eosinophil trafficking during healthy conditions (11) and inflammation (12).…”

Section: Introductionmentioning

confidence: 99%

Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model

Huang

Zhang

et al. 2020

Front. Immunol.

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Schistosomiasis is a severe public health problem, which can cause tissue fibrosis and can even be fatal. Previous studies have proven that galectins and different kinds of cells involve in the regulation of tissue fibrosis process. In this study, outbred Kunming mice were infected with Schistosoma japonicum (S. japonicum). Our results showed that compared with uninfected mice, there were severe egg granulomatous inflammation and tissue fibrosis in the livers, spleens, and large intestines of S. japonicum-infected mice at 8 weeks post-infection (p.i.), and the number of eosinophils by hematoxylin and eosin staining and CD68 macrophage-positive area by immunohistochemical staining were significantly increased. Detected by using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR), at 8 weeks after S. japonicum infection, the mRNA expression levels of galectin (Gal)-1, Gal-3, CD69, eosinophil protein X (EPX), and chitinase 3-like protein 3 (Ym1) were significantly increased in liver, spleen, and large intestine; eotaxin-1 (CCL11) and eosinophil cationic protein were significantly increased in both liver and spleen; eotaxin-2 (CCL24) and Arginase1 (Arg1) were significantly increased in both spleen and large intestine; and CD200R was significantly increased in both liver and large intestine. However, interleukin (IL)-1ß and inducible nitric oxide synthase (iNOS) were only significantly increased in liver. The M2/M1 ratio of CD200R/CD86 genes was significantly increased in liver, and ratios of Ym1/IL-1β and Ym1/iNOS were significantly increased in liver, spleen, and large intestine of S. japonicum-infected mice. Ex vivo study further confirmed that the levels of Gal-1, Gal-3, CD200R, Arg1, and Ym1 were significantly increased, and the ratios of CD200R/CD86 and Ym1/IL-1β were significantly increased in peritoneal macrophages isolated from S. japonicum-infected mice at 8 weeks p.i. In addition, correlation analysis showed that significant positive correlations existed between mRNA levels of Gal-1/Gal-3 and EPX in liver, between Gal-3 and Ym1 in both liver and large intestine, and between Gal-3 and CD200R in peritoneal macrophages of S. japonicum-infected mice. Our data suggested that Gal-1, Gal-3, eosinophils, and macrophages are likely involved in the development of egg granulomatous response and fibrosis induced by S. japonicum infection.

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Markers of atopic dermatitis, allergic rhinitis and bronchial asthma in pediatric patients: correlation with filaggrin, eosinophil major basic protein and immunoglobulin E

Cited by 27 publications

References 33 publications

Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitis

Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitis

Synergy of Interleukin (IL)-5 and IL-18 in eosinophil mediated pathogenesis of allergic diseases

Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model

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