Markers of cartilage and synovial metabolism in joint fluid and serum of patients with chondromalacia of the patella

Väätäinen, Urho; Lohmander, L. Stefan; Thonar, Eugene J-M.A.; Hongisto, Tero; Ågren, Ulla M.; Rönkkö, Seppo; Jaroma, H; Kosma, Veli‐Matti; Tammi, Markku; Kirviranta, Ilkka

doi:10.1053/joca.1997.0101

Cited by 38 publications

(5 citation statements)

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“…In interpreting these results, it should be noted that the levels of cytokine used in our short‐term studies were probably much higher than the levels seen in synovial fluid in vivo. Reported concentrations of IL‐1β range from 1 pg/ml in patients with chondromalacia of the patella (27) to 20 pg/ml in patients with rheumatoid arthritis (28); TNFα concentrations can range from 40 pg/ml to 250 pg/ml in rheumatoid arthritis (28). Therefore, the absolute amounts of matrix loss are not applicable to in vivo joint injuries.…”

Section: Discussionmentioning

confidence: 99%

Proteoglycan degradation after injurious compression of bovine and human articular cartilage in vitro: Interaction with exogenous cytokines

Patwari

Cook

DiMicco

et al. 2003

Arthritis & Rheumatism

152

163

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Objective. Traumatic joint injury leads to an increased risk of osteoarthritis (OA), but the progression to OA is not well understood. We undertook this study to measure aspects of proteoglycan (PG) degradation after in vitro injurious mechanical compression, including up-regulation of enzymatic degradative expression and cytokine-stimulated degradation.Methods. Articular cartilage tissue explants were obtained from newborn bovine femoropatellar groove and from adult normal human donor knee and ankle tissue. Following injurious compression of the cartilage, matrix metalloproteinase 3 (MMP-3) and MMP-13 messenger RNA (mRNA) expression levels were measured by Northern analysis, and PG loss to the medium after cartilage injury was measured in the presence and absence of added exogenous cytokine (interleukin-1␣ [IL-1␣] or tumor necrosis factor ␣ [TNF␣]).Results. During the first 24 hours after injury in bovine cartilage, MMP-3 mRNA levels increased 10-fold over the levels in control cartilage (n ‫؍‬ 3 experiments), whereas MMP-13 mRNA levels were unchanged. PG loss was significantly increased after injury, but only by 2% of the total PG content and only for the first 3 days following injury. However, compared with injury alone or cytokine treatment alone, treatment of injured tissue with either 1 ng/ml IL-1␣ or 100 ng/ml TNF␣ caused marked increases in PG loss (35% and 54%, respectively, of the total cartilage PG content). These interactions between cytokine treatment and injury were statistically significant. In human knee cartilage, the interaction was also significant for both IL-1␣ and TNF␣, although the magnitude of increase in PG loss was lower than that in bovine cartilage. In contrast, in human ankle cartilage, there was no significant interaction between injury and IL-1␣.Conclusion. The cytokines IL-1␣ and TNF␣ can cause a synergistic loss of PG from mechanically injured bovine and human cartilage. By attempting to incorporate interactions with other joint tissues that may be sources of cytokines, in vitro models of mechanical cartilage injury may explain aspects of the interactions between mechanical forces and degradative pathways which lead to OA progression.

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Section: Discussionmentioning

confidence: 99%

Proteoglycan degradation after injurious compression of bovine and human articular cartilage in vitro: Interaction with exogenous cytokines

Patwari

Cook

DiMicco

et al. 2003

Arthritis & Rheumatism

152

163

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“…Human MMP-3, -9, and -13 were chosen based on their established importance in OA[11,19,20]. To achieve comparable results between the different enzymes, the amount of active enzyme in each assay was normalized using the specific activity (Units of enzyme activity per weight) provided by the manufacturer (Supplementary information 1).…”

Section: Methodsmentioning

confidence: 99%

In-vitro and in-vivo imaging of MMP activity in cartilage and joint injury

Fukui

Tenborg

Yik

et al. 2015

Biochemical and Biophysical Research Communications

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Non-destructive detection of cartilage-degrading activities represents an advance in osteoarthritis (OA) research, with implications in studies of OA pathogenesis, progression, and intervention strategies. Matrix metalloproteinases (MMPs) are principal cartilage degrading enzymes that contribute to OA pathogenesis. MMPSense750 is an in-vivo fluorimetric imaging probe with the potential to continuously and non-invasively trace real-time MMP activities, but its use in OA-related research has not been reported. Our objective is to detect and characterize the early degradation activities shortly after cartilage or joint injury with MMPSense750. We determined the appropriate concentration, assay time, and linear range using various concentrations of recombinant MMPs as standards. We then quantified MMP activity from cartilage explants subjected to either mechanical injury or inflammatory cytokine treatment in-vitro. Finally, we performed invivo MMP imaging of a mouse model of post-traumatic OA. Our in-vitro results showed that the optimal assay time was highly dependent on the MMP enzyme. In cartilage explant culture media, mechanical impact or cytokine treatment increased MMP activity. Injured knees of mice showed significantly higher fluorescent signal than uninjured knees. We conclude that MMPSense750 detects human MMP activities and can be used for in-vitro study with cartilage, as well as in-vivo studies of knee injury, and can offering real-time insight into the degradative processes that occurring within the joint before structural changes become evident radiographically.

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“…Ein standardisiertes Protokoll zur Gewinnung und Messung von Markermolekülen existiert bislang nicht. Vielfach werden Serum-, andererseits auch unverdünnte oder verdünnte Proben zur Messung herangezogen [28,42]. In unserer Studie wurden ausschließlich standardisierte Verdünnungen gewählt, um Einflüsse durch begleitende Synovitis oder Erguss auszuschließen.…”

Section: Magnetresonanztomografieunclassified

Osteochondrale Molekülmarker als quantitative Verlaufsparameter nach matrixgekoppelter autologer Chondrozytentransplantation CaRes®

Gravius

Schneider²,

Mumme³

et al. 2007

Z Orthop Unfall

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Under consideration of missing correlations to clinical parameters (IKDC/MRI) non-specific osteochondral marker proteins of the synovial fluid cannot be used without further scrutiny to document changes in cartilage and osseous metabolism following matrix-supported ACI over the time of 52 weeks objectively. The drop of the concentrations below preoperative levels at 6 weeks can possibly be explained by the reduced traumatization of the joint with the CaRes procedure compared to the classic ACI. Specific markers for cartilage metabolism should be defined to permit a direct and objective comparison of the various conservative and operative methods presently available for the treatment of chondral lesions of the knee joint.

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Markers of cartilage and synovial metabolism in joint fluid and serum of patients with chondromalacia of the patella

Abstract: The changes in the release and activity of these marker molecules from serum and synovial fluid may reflect changes in the metabolism of articular cartilage and synovium in CM, that are consistent with those observed in early-stage tibiofemoral cartilage changes in OA.

Cited by 38 publications

References 0 publications

Proteoglycan degradation after injurious compression of bovine and human articular cartilage in vitro: Interaction with exogenous cytokines

Proteoglycan degradation after injurious compression of bovine and human articular cartilage in vitro: Interaction with exogenous cytokines

In-vitro and in-vivo imaging of MMP activity in cartilage and joint injury

Osteochondrale Molekülmarker als quantitative Verlaufsparameter nach matrixgekoppelter autologer Chondrozytentransplantation CaRes®

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