Groundwater long-term monitoring (LTM) isrequired to assess human health and environmental risk of residual contaminants after active groundwater remediation activities are completed. However, LTM can be costly because of the large number of sampling locations that exist at a site from previous site characterization and remediation activities. The cost of LTM may be reduced by identifying redundant sampling locations. However, care must be taken so that the elimination of specific individual wells from the monitoring network does not result in unacceptable levels of data loss and errors. An ant colony optimization (ACO) algorithm is proposed to identify optimal sampling networks that minimize the number of monitoring locations while maintaining the overall data loss below a given threshold. ACO is inspired by the ability of an ant colony to identify the shortest route between its nest and a food source through indirect communication and positive feedback. Metrics for quantifying well redundancy and overall data loss after optimization are quantified and used in the ACO heuristics. To demonstrate its effectiveness, the ACO developed for LTM optimization is applied to a case study with 30 existing monitoring wells. The LTM optimization problem was solved using different data loss thresholds to identify solutions with 27 to 21 wells remaining in the LTM network. Contour mapping of the contaminant plume using the remaining wells show that the ACO solutions are effective and practical. These results demonstrated that ACO is a promising method for solving LTM optimization problems.