Marrow Distribution in Rat Femurs Determined by Cell Enumeration and Fe59 Labeling.

LoBue, Joseph; Dornfest, Burton S.; Gordon, Albert S.; Hurst, Josephine M.; Quastler, Henry

doi:10.3181/00379727-112-28250

Cited by 43 publications

(13 citation statements)

References 3 publications

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“…2). It therefore appears that the enzyme is associated with a more immature cell than the erythroblast which is the earliest benzidine-positive cell (10). Other studies (24,27,28) have shown that after the administration of ESF to polycythemic mice proerythroblasts appear in the spleen within 12-24 hr, while erythroblasts are not seen until the 2nd day.…”

Section: Effect Of Friend Virus Infectionmentioning

confidence: 89%

“…Benzidine staining does not permit identification of very early erythroid precursors, i.e. proerythroblasts, which lack sufficient amounts of hemoglobin to be detected by the stain (10).…”

Section: Methodsmentioning

confidence: 99%

“…The pulp was removed from the other portion of the spleen and was uniformly suspended in isologous serum with a rubber-bulbed glass pipette. The hemoglobin-containing nucleated red cells in this suspension were enumerated from smears stained by the benzidine technique (10). The count was expressed as the percentage of total nucleated cells which were benzidine-positive.…”

Section: Methodsmentioning

confidence: 99%

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Nucleoside deaminase: an enzymatic marker for stress erythropoiesis in the mouse

Rothman¹,

Zanjani²,

Gordon³

et al. 1970

J. Clin. Invest.

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A B S T R A C T The level of nucleoside deaminase was determined in extracts of mouse tissues obtained during a period of accelerated erythropoiesis induced by hypoxia, hemorrhage, or the injection of phenylhydrazine. Under these conditions a striking (10-to 100-fold) elevation of the enzyme activity occurred in the spleen. Similar results were obtained with the injection of purified erythropoietin. In control animals, only a trace of nucleoside deaminase activity was detected in the blood. During the reticulocyte response which followed erythropoietic stimulation, there was a sharp increase in the blood level of nucleoside deaminase, which rose up to 120 times that of control animals. By differential centrifugation, the enzyme was localized to the reticulocyte-rich fraction. Erythrocyte nucleoside deaminase remained elevated even after the reticulocyte count had fallen to normal in the phenylhydrazine-treated mice or to zero after the cessation of hypoxia. There was a very gradual decline in the enzyme activity in the blood which fell to the barely detectable control levels about 45 days after the initial reticulocyte response, a time period which corresponds to the survival of the mouse red blood cell. The persistence of high levels of nucleoside deaminase for the full life span of a generation of erythrocytes formed during stress, viewed in contrast to the virtual absence of the enzyme from normal erythrocytes of all ages, represents an enzymatic difference between the normal red blood cell and the cell produced under conditions of accelerated erythropoiesis.

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Section: Effect Of Friend Virus Infectionmentioning

confidence: 89%

“…Benzidine staining does not permit identification of very early erythroid precursors, i.e. proerythroblasts, which lack sufficient amounts of hemoglobin to be detected by the stain (10).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Nucleoside deaminase: an enzymatic marker for stress erythropoiesis in the mouse

Rothman¹,

Zanjani²,

Gordon³

et al. 1970

J. Clin. Invest.

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show abstract

“…Blood and spleen reticulocytes were counted by the direct, smear method, using cresyl blue, up to 1000 red cells. Erythroblasts were counted as a percentage of 500 nucleated cells on smears of the spleen strained with benzidine and May-Griinwald-Giemsa stains [12].…”

Section: Cellsmentioning

confidence: 99%

Isolation and Characterization of Highly Purified Globin Messenger RNA from Anaemic-Rabbit Spleen

Nokin¹,

Burny

Cleuter³

et al. 1975

Eur J Biochem

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Highly purified globin messenger RNA has been isolated from the erythropoietic spleen of anaemic rabbits. This RNA, which sediments at 9‐S, directs the synthesis of α and β rabbit globin chains in a Krebs II ascites cell‐free system. Globin accounts for more than 90% of the cell‐free synthesized proteins. Spleen 9‐S RNA is, however, about 30% less effective on a weight basis than blood reticulocyte 9‐S RNA in directing globin synthesis in the cell‐free system. When analysed on polyacrylamide gels, spleen 9‐S RNA seems to be somewhat more heterogeneous in size than blood reticulocyte 9‐S RNA. Spleen globin mRNA contains a polyadenylic segment which is at least as long as the poly(A) tract of globin mRNA from blood reticulocytes.

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“…The present experiments were done on a clone designated (12), then counterstained with Leischman, and the percent of benzidine-positive (B+) cells was determined.…”

Section: Methodsmentioning

confidence: 99%

Hemoglobin Synthesis in Murine Virus-Induced Leukemic Cells In Vitro: Stimulation of Erythroid Differentiation by Dimethyl Sulfoxide

Friend

Scher

Holland

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

1,222

490

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Cells of a cloned line of murine virusinduced erythroleukemia were stimulated to differentiate along the erythroid pathway by dimethyl sulfoxide at concentrations that did not inhibit growth. A rise in the number of benzidine-positive normoblasts was accompanied by increased synthesis of heme and hemoglobin and a decrease in the malignancy of the cells. This action of dimethyl sulfoxide, which was reversible, may represent the derepression of leukemic cells to permit their maturation.The opportunity to explore the possibility that leukemia is a disease resulting from a block in the process of maturation of hematopoietic cells has been provided by established tissue culture lines of murine virus-induced erythroleukemic cells (1-3). These cells, which grow in suspension, have continued to exhibit a limited degree of differentiation along the erythroid line throughout their 4-year serial passage history. Although they synthesize hemoglobin (4, 5), they are malignant as tested by bioassay in syngeneic hosts. They produce virus which, although low in leukemogenic activity, is a highly effective immunizing agent (6).During the course of studies to determine the effect of superinfecting these cells with Friend leukemia virus, dimethyl sulfoxide (DMS0) was added to the medium. DMS0 had been demonstrated to enhance infectivity of both poliovirus RNA (7) and mengovirus RNA (8), as well as transformation by polyoma virus (9). It is also known to stabilize enveloped viruses (10). The wide range of biological activities of DMSO has been described (11). The present report de-scribes an effect of DMSO on the differentiation of established lines of murine virus-induced leukemic cells and illustrates still another property of this compound.In the dose-response experiments initially set up to determine the toxicity of DMSO on the leukemic cell lines, a striking effect on the differentiation of these cells was noted. Of the cells allowed to grow in medium containing 2% DMS0 for 4 days, a majority of the erythroblasts had matured to normoblasts which stained benzidine-positive (B+). The increase in the number of cells maturing along the erythroid series in DMS0-containing medium was accompanied by an increase in the amount of hemoglobin synthesized. MATERIALS AND METHODSThe origin of the cell lines of murine Friend leukemia virusinduced leukemic cells and their clonal derivatives has been described previously (1,2). Methods for maintaining the cell culture, cloning on semisoft agar, and the medium were also detailed in these earlier reports.The present experiments were done on a clone designated 707, in which 1-2% of the cells are B+ (5). Cells were generally seeded at a concentration of 105 per ml (except where otherwise indicated) either in 32-oz. (900 ml) prescription bottles containing 60 ml of medium or in Falcon plastic Petri dishes (60 X 15 mm) containing 10 ml of medium. Dehydrated Eagle's basal medium diluted with Earle's balanced salt solution and supplemented with 15% fetal calf serum was used. The cultures were ...

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Marrow Distribution in Rat Femurs Determined by Cell Enumeration and Fe59 Labeling.

Cited by 43 publications

References 3 publications

Nucleoside deaminase: an enzymatic marker for stress erythropoiesis in the mouse

Nucleoside deaminase: an enzymatic marker for stress erythropoiesis in the mouse

Isolation and Characterization of Highly Purified Globin Messenger RNA from Anaemic-Rabbit Spleen

Hemoglobin Synthesis in Murine Virus-Induced Leukemic Cells In Vitro: Stimulation of Erythroid Differentiation by Dimethyl Sulfoxide

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