Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential

Abstract

We report here the isolation of a population of nontransformed pluripotent human cells from bone marrow after a unique expansion/selection procedure. This procedure was designed to provide conditions resembling the in vivo microenvironment that is home for the mostprimitive stem cells. Marrow-adherent and -nonadherent cells were co-cultured on fibronectin, at low oxygen tension, for 14 days. Colonies of small adherent cells were isolated and further expanded on fibronectin at low density, low oxygen tension with 2% fetal bovine serum. They expressed high levels of CD29, CD63, CD81, CD122, CD164, hepatocyte growth factor receptor (cMet), bone morphogenetic protein receptor 1B (BMPR1B), and neurotrophic tyrosine kinase receptor 3 (NTRK3) and were negative for CD34, CD36, CD45, CD117 (cKit) and HLA-DR. The embryonic stem cell markers Oct-4 and Rex-1, and telomerase were expressed in all cultures examined. Cell-doubling time was 36 to 72 hours, and cells have been expanded in culture for more than 50 population doublings. This population of cells was consistently isolated from men

Very small embryonic-like (VSEL) stem cells in adult organs and their potential role in rejuvenation of tissues and longevity

“…First, expressions of typical PSC markers such as SSEA-1, Oct-4, and Nanog were reported in BM-derived SCs isolated using various strategies. These early embryonic transcription factors that are characteristic for ES cells and epiblast-derived cells were demonstrated at the protein and/or mRNA levels in VSELs, MAPCs, MSCs (in particular the SD fraction), and MIAMI cells (D'Ippolito et al, 2004;Jiang et al, 2002;Kucia et al, 2006a;Lamoury et al, 2006;Pochampally et al, 2004). Second, the contribution of BM-derived cells for regenerating multiple non-hematopoietic organs and tissues indirectly suggests the existence of pluripotent or multipotent SCs in BM.…”

“…Evidence accumulated that BM contains heterogeneous populations of hematopoietic (H)SCs and non-HSCs. These non-HSCs are described in the literature as: endothelial progenitor cells (EPCs) (Asahara et al, 1997;Shi et al, 1998); mesenchymal (M)SCs (Dominici et al, 2006, Peister et al, 2004Prockop, 1997); multipotent adult progenitor cells (MAPCs) (Jiang et al, 2002); marrowisolated adult multilineage inducible (MIAMI) cells (D'Ippolito et al, 2004); and multipotent adult (MA)SCs (Beltrami et al, 2007). Moreover, BM could also be postulated as a potential source of precursors of germ cells (GCs) (oocytes and spermatogonial cells) (Johnson et al, 2005;Nayernia et al, 2006).…”

Concise Review: Culture Mediated Changes in Fate and/or Potency of Stem Cells

“…Since the original description of rodent MAPC, other groups and we have published that cells with broader differentiation potential can also be culture-isolated from human tissues, including BM (MAPC, marrow isolated adult multili- [48][49][50][51][52]. The cell surface phenotype, TF expression profile and differentiation potential of these cells is summarized in Table 1.…”

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

“…They could also be derived as immortalized cell lines As mentioned above, recent research indicates that BM in addition to HSC also contains a heterogeneous population of nonhematopoietic stem cells and some of these cells were proposed to be PSC. These cells have been variously described in the literature as (1) MSC (Prockop, 1997;Peister et al, 2004), (2) MAPC (Jiang et al, 2002), (3) MIAMI cells (DЈIppolito et al, 2004), and (4) USSC (Kogler et al, 2004). Recently, we isolated from BM a population of VSEL stem cells (Kucia et al, 2006b).…”

“…Because VSEL show tropism to stromal cells and undergo emperipolesis in cocultures with bone marrowderived fibroblasts (Kucia et al, 2005), we postulate that they could be coisolated with the adherent fractions of BM cells that were described as mesenchymal stem cells (MSC; Prockop, 1997), multipotent adult progenitor cells (MAPC; Jiang et al, 2002), or marrow-isolated adult multilineage inducible (MIAMI) cells (DЈIppolito et al, 2004).…”