MaSERK1 Gene Expression Associated with Somatic Embryogenic Competence and Disease Resistance Response in Banana (Musa spp.)

Huang, Xia; Lu, Xin-Jiang; Zhao, Jietang; Chen, Jiekai; Dai, Xuemei; Xiao, Wang; Chen, Yaping; Chen, Yun-Feng; Huang, Xue-Lin

doi:10.1007/s11105-009-0150-z

Cited by 49 publications

(24 citation statements)

References 29 publications

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“…The expression analysis of AcSERK1 in the nonembryogenic callus and organs, such as leaf, stem, and calyx, showed that AcSERK1 expression occurred at lower levels than those observed in embryogenic tissues, thus suggesting that AcSERK1 is associated with SE induction in pineapple, but it belongs to non-featured expression in embryogenic callus. This result was different with those obtained from carrot (Schmidt et al 1997), Arabidopsis , and banana (Huang et al 2010), where SERK was only expressed in embryogenic callus. However, the high expression level of AcSERK1 during the acquisition of embryogenic competence and the development of global embryo is similar to the SERKs above.…”

Section: Discussioncontrasting

confidence: 91%

“…These results indicated that SERK plays an important role in SE, and it can be used as a marker of embryogenic competence. Dissimilar expression patterns were found in soybean and banana (Huang et al 2010;Yang et al 2011). It suggested that SERK may be involved in plant development, disease resistance, or other procedures (Alam et al 2010;Huang et al 2010;Yang et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Cloning and Molecular Characterization of a SERK Gene Transcriptionally Induced During Somatic Embryogenesis in Ananas comosus cv. Shenwan

Cheng-hou

et al. 2011

Plant Mol Biol Rep

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A somatic embryogenesis receptor-like kinase (SERK) gene, designated as AcSERK1, was isolated from pineapple (Ananas comosus cv. Shenwan). AcSERK1 shared all the characteristic domains of the SERK family, including five leucine-rich repeats, one proline-rich region motif, transmembrane domain, and kinase domains. Somatic embryogenic cultures of pineapple were established following transfer of callus cultures to Murashige and Skoog (1962) medium containing 2,4-dichlorophenoxyacetic acid. The role of AcSERK1 during establishment of somatic embryogenesis in culture was investigated. The AcSERK1 was highly expressed during embryogenic competence acquisition and global embryo formation in culture. These findings were obtained along with morphological changes in callus cultures exhibiting embryogenic potential. Overall, levels of expression of AcSERK1 were lower in nonembryogenic tissues and organs than in embryogenic callus. In situ hybridization analysis revealed that AcSERK1 expression was detected in embryogenic tissues, including single competent cells, meristematic centers wherein embryogenic structures are formed, and global embryos. These results suggested that AcSERK1 expression was associated with induction of somatic embryogenesis and that it could be used as a potential marker gene to monitor the transition of pineapple callus tissues into competent and embryogenic cells and tissues.

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Section: Discussioncontrasting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Cloning and Molecular Characterization of a SERK Gene Transcriptionally Induced During Somatic Embryogenesis in Ananas comosus cv. Shenwan

Cheng-hou

et al. 2011

Plant Mol Biol Rep

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“…This is consistent with previous research that demonstrated that OsSERK1 showed variable expression levels in different tissues, as well as by the host defense against fungal infection and positively regulated somatic embryogenesis of cultured cells (Hu et al, 2005). Moreover, MaSERK1 was found to be associated with disease resistance response and somatic embryogenic competence (Huang et al, 2010). However, further analysis of the functions of MhSERK1 and MhdSERK1 in different organs and tissues is required.…”

Section: Discussionsupporting

confidence: 91%

Cloning and characterization of the SERK1 gene in triploid Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang] and a tetraploid hybrid strain

et al. 2015

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Cloning and characterization of theABSTRACT. This study aims to explore the roles of somatic embryogenesis receptor-like kinase (SERK) in Malus hupehensis (Pingyi Tiancha). The fulllength sequences of SERK1 in triploid Pingyi Tiancha (3n) and a tetraploid hybrid strain 33# (4n) were cloned, sequenced, and designated as MhSERK1 and MhdSERK1, respectively. Multiple alignments of amino acid sequences were conducted to identify similarity between MhSERK1 and MhdSERK1 and SERK sequences in other species, and a neighbor-joining phylogenetic tree was constructed to elucidate their phylogenetic relations. Expression levels of MhSERK1 and MhdSERK1 in different tissues and developmental stages were investigated using quantitative real-time PCR. The coding sequence lengths of MhSERK1 and MhdSERK1 were 1899 bp (encoding 632 amino acids) and 1881 bp (encoding 626 amino acids), respectively. Sequence analysis demonstrated that MhSERK1 and MhdSERK1 display high similarity to SERKs in other species, with a conserved intron/exon structure that is unique to members of the SERK 14577 Roles of SERK1 in Pingyi Tiancha and a hybrid strain ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 14576-14586 (2015) family. Additionally, the phylogenetic tree showed that MhSERK1 and MhdSERK1 clustered with orange CitSERK (93%). Furthermore, MhSERK1 and MhdSERK1 were mainly expressed in the reproductive organs, in particular the ovary. Their expression levels were highest in young flowers and they differed among different tissues and organs. Our results suggest that MhSERK1 and MhdSERK1 are related to plant reproduction, and that MhSERK1 is related to apomixis in triploid Pingyi Tiancha.

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“…OsSERK1 is thought to be partially responsible for mediating defense signal transduction and has a basic role in rice somatic embryogenesis (Hu et al, 2005). More homologs of SERK have been identified in several plant species, including Dactylis glomerata (Somleva et al, 2000), Medicago truncatula (Nolan et al, 2003(Nolan et al, , 2009, Ocotea catharinensis (Santa-Catarina et al, 2004), Helianthus annuus (Thomas et al, 2004), Theobroma cacao (Santos et al, 2005), Citrus unshiu (Shimada et al, 2005), Triticum aestivum (Singla et al, 2008), Vitis vinifera (Schellenbaum et al, 2008), Solanum tuberosum (Sharma et al, 2008), Cocos nucifera (Perez-Núñez et al, 2009), Lactuca sativa (Santos et al, 2009), Rosa hybrida (Zakizadeh et al, 2010), Musa acuminata (Huang et al, 2010), Secale cereale (Gruszczyńska and Rakoczy-Trojanowska, 2011), Glycine max (Yang et al, 2011), Cyclamen persicum (Savona et al, 2012), Ananas comosus (Ma et al, 2012a,b), and Cyrtochilum loxense (Cueva et al, 2012). These reports all point to SERK as a specific marker of embryogenic competence during morphogenesis induction.…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Yw¹,

Yj²,

Fc³

2014

Genet. Mol. Res.

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ABSTRACT. Somatic embryogenesis receptor-like kinase (SERK) genes have been found to be involved in the somatic embryogenesis of several plant species. We identified and characterized 5 PhSERK genes in the Phalaenopsis orchid. The amino acid sequences of PhSERKs and other SERK proteins are highly conserved, with the highest homology observed in the leucine-rich repeat-receptor-like kinase domain. All 5 PhSERKs were expressed in all Phalaenopsis organs examined (root, leaf, shoot apical meristem, and flower), with the strongest expression, particularly for PhSERK1 and 3, in the shoot apical meristem of mature plants. Expression of all PhSERKs was downregulated during early floral bud development and was upregulated gradually until the semiopen flower stage was reached. All 5 PhSERKs were expressed during both seed germination and protocorm-like-body (PLB) development. In germinated seeds, quantitative real-time PCR revealed upregulation of all PhSERKs except PhSERK4 at 1 week and downregulation after 4 weeks. The 5 PhSERKs were differentially expressed in the early stage Characterization of genes from Phalaenopsis of PLB development and maintained substantial levels during PLB formation, with PhSERK1 and 5 upregulated 1 week after culture and PhSERK2, 3, and 4 downregulated over this period. Because physical wounding of PLB stimulates secondary PLB formation, the PhSERK5 expression peak at week 3 coincided with visible and fully developed secondary PLBs. PhSERK5 may be important in PLB induction and subsequent development. Our PhSERK expression analysis revealed that these genes have a broad role during orchid plant development.

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MaSERK1 Gene Expression Associated with Somatic Embryogenic Competence and Disease Resistance Response in Banana (Musa spp.)

Cited by 49 publications

References 29 publications

Cloning and Molecular Characterization of a SERK Gene Transcriptionally Induced During Somatic Embryogenesis in Ananas comosus cv. Shenwan

Cloning and Molecular Characterization of a SERK Gene Transcriptionally Induced During Somatic Embryogenesis in Ananas comosus cv. Shenwan

Cloning and characterization of the SERK1 gene in triploid Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang] and a tetraploid hybrid strain

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

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