Mash1 efficiently reprograms rat astrocytes into neurons

Ding, Daofang; Xu, Leqin; Xu, Hao; Li, Xiaofeng; Liang, Qianqian; Zhao, Yongjian; Wang, Yongjun

doi:10.4103/1673-5374.125326

Cited by 3 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with previous studies, proneuronal TFs, including Ngn1 and NeuroD, were mainly expressed in neuronal precursors and immature neurons to promote neurogenesis, and decreased in the late stage of neuronal differentiation of MSCs [62,63]. Mash1 not only promoted neuronal differentiation, but also maintained the neuronal phenotype, and therefore, it was expressed in immature and mature neurons [64,65]. Similar to these studies, the expression levels of Mash1 were increasing throughout the differentiation process in each group, except in the 60 mg/l TMP group where its expression decreased, which may be associated with the anti-aging effect exhibited by 60 mg/l TMP.…”

Section: Discussionsupporting

confidence: 86%

Anti-aging effects exerted by Tetramethylpyrazine enhances self-renewal and neuronal differentiation of rat bMSCs by suppressing NF-kB signaling

Song

Dai

et al. 2019

Bioscience Reports

View full text Add to dashboard Cite

In order to improve the therapeutic effects of mesenchymal stem cell (MSC)-based therapies for a number of intractable neurological disorders, a more favorable strategy to regulate the outcome of bone marrow MSCs (bMSCs) was examined in the present study. In view of the wide range of neurotrophic and neuroprotective effects, Tetramethylpyrazine (TMP), a biologically active alkaloid isolated from the herbal medicine Ligusticum wallichii, was used. It was revealed that treatment with 30–50 mg/l TMP for 4 days significantly increased cell viability, alleviated senescence by suppressing NF-κB signaling, and promoted bMSC proliferation by regulating the cell cycle. In addition, 40–50 mg/l TMP treatment may facilitate the neuronal differentiation of bMSCs, verified in the present study by presentation of neuronal morphology and expression of neuronal markers: microtubule-associated protein 2 (MAP-2) and neuron-specific enolase (NSE). The quantitative real-time polymerase chain reaction (qRT-PCR) revealed that TMP treatment may promote the expression of neurogenin 1 (Ngn1), neuronal differentiation 1 (NeuroD) and mammalian achaete–scute homolog 1 (Mash1). In conclusion, 4 days of 40–50 mg/l TMP treatment may significantly delay bMSC senescence by suppressing NF-κB signaling, and enhancing the self-renewal ability of bMSCs, and their potential for neuronal differentiation.

show abstract

Section: Discussionsupporting

confidence: 86%

Anti-aging effects exerted by Tetramethylpyrazine enhances self-renewal and neuronal differentiation of rat bMSCs by suppressing NF-kB signaling

Song

Dai

et al. 2019

Bioscience Reports

View full text Add to dashboard Cite

show abstract

“…Direct reprogramming of somatic cells has a promising outlook to be used in cell‐replacement therapy for the treatment of neurodegenerative disorders and neuronal trauma. Though several studies investigated the feasibility of astrocyte conversion into neuronal cells in vitro and in vivo (Berninger et al, ; Corti et al, ; Ding et al, ; Hack et al, ; Heinrich et al, ; Liu et al, ; Torper et al, ), little is known on the conversion of adult astrocytes into NSCs (Aravantinou‐Fatorou et al, ; Corti et al, ; Ghasemi‐Kasman et al, ; Niu et al, ).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…There are many reports on in vitro or in vivo reprogramming of astroglial cells into neuronal cells using different transcription factors such as Ascl1 (Ding et al, 2014;Liu et al, 2015), Brn2, Ascl1, and Myt1l (Torper et al, 2013), Oct4, Ascl1, Sox2, and Nanog (Corti et al, 2012), Neurog2 or Dlx2 (Heinrich et al, 2010), Pax6 (Hack et al, 2005), and Neurog2 and Ascl1 (Berninger et al, 2007). Moreover, it was indicated that induction of Sox2 could reprogram adult mouse brain astrocytes into Ascl-positive intermediate progenitors, which proliferate and generate doublecortin (Dcx)-positive neuroblasts, in vivo (Niu et al, 2015).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Generation of neural stem cells from adult astrocytes by using a single reprogramming factor

Zarei-Kheirabadi

Hesaraki

Shojaei

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

Generating neural stem cells (NSCs) from astroglia as an abundant cell type in the mammalian brain has a promising outlook to be used in cell‐replacement therapy for treatment of neurodegenerative disorders and neuronal trauma. However, little is known about a single reprogramming factor that may lead to the generation of induced NSCs (iNSCs) from adult brain‐derived astrocytes in the absence of extrinsic inductive signals. Here, we show that zinc‐finger nuclear protein Zfp521 alone is sufficient for converting the adult mouse brain‐derived astrocytes into iNSCs. In vitro, Zfp521‐iNSCs demonstrated long‐term self‐renewal and multipotency and expressed related markers. Moreover, single‐seeded iNSCs were able to produce NSC colonies. These results suggest that application of Zfp521 to generate iNSCs could be regarded as a new approach for conversion of resident astrocytes into iNSCs in cell therapy for in vivo treatment of neural injuries.

show abstract

Glia–neuron interactions in the mammalian retina

Vecino

Rodríguez

Ruzafa

et al. 2016

Progress in Retinal and Eye Research

642

533

View full text Add to dashboard Cite

The mammalian retina provides an excellent opportunity to study glia-neuron interactions and the interactions of glia with blood vessels. Three main types of glial cells are found in the mammalian retina that serve to maintain retinal homeostasis: astrocytes, Müller cells and resident microglia. Müller cells, astrocytes and microglia not only provide structural support but they are also involved in metabolism, the phagocytosis of neuronal debris, the release of certain transmitters and trophic factors and K(+) uptake. Astrocytes are mostly located in the nerve fibre layer and they accompany the blood vessels in the inner nuclear layer. Indeed, like Müller cells, astrocytic processes cover the blood vessels forming the retinal blood barrier and they fulfil a significant role in ion homeostasis. Among other activities, microglia can be stimulated to fulfil a macrophage function, as well as to interact with other glial cells and neurons by secreting growth factors. This review summarizes the main functional relationships between retinal glial cells and neurons, presenting a general picture of the retina recently modified based on experimental observations. The preferential involvement of the distinct glia cells in terms of the activity in the retina is discussed, for example, while Müller cells may serve as progenitors of retinal neurons, astrocytes and microglia are responsible for synaptic pruning. Since different types of glia participate together in certain activities in the retina, it is imperative to explore the order of redundancy and to explore the heterogeneity among these cells. Recent studies revealed the association of glia cell heterogeneity with specific functions. Finally, the neuroprotective effects of glia on photoreceptors and ganglion cells under normal and adverse conditions will also be explored.

show abstract

Mash1 efficiently reprograms rat astrocytes into neurons

Cited by 3 publications

References 53 publications

Anti-aging effects exerted by Tetramethylpyrazine enhances self-renewal and neuronal differentiation of rat bMSCs by suppressing NF-kB signaling

Anti-aging effects exerted by Tetramethylpyrazine enhances self-renewal and neuronal differentiation of rat bMSCs by suppressing NF-kB signaling

Generation of neural stem cells from adult astrocytes by using a single reprogramming factor

Glia–neuron interactions in the mammalian retina

Contact Info

Product

Resources

About