Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma

Dzinic, Sijana H.; Zaid, Masliza; Bernardo, M. Margarida; Vranić, Semir; Beydoun, H.; Nahra, Nadine; Alijagic, Amra; Harajli, Deanna; Pang, Aaron; Saliganan, Dan M.; Rahman, Abid M.; Skenderi, Faruk; Hasanbegović, Berisa; Dyson, Gregory; Beydoun, Rafic; Shen, Sheng

doi:10.1371/journal.pone.0215089

Cited by 6 publications

(5 citation statements)

References 47 publications

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“…( Immunohistochemistry experiments were carried out as previously described. 37 Immunostained positivity was defined and classified according to the respective protein expression in either the nucleus only, cytoplasm only, cell membrane only and/or combined; blindly confirmed by the pathologist. Microscopic images were processed using a Nikon microscope.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Circulating microRNA‐590‐5p functions as a liquid biopsy marker in non‐small cell lung cancer

et al. 2020

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Despite the availability of various diagnostic procedures, a tissue biopsy is still indispensable for the routine diagnosis of lung cancer. However, inaccurate diagnoses can occur, leading to inefficient cancer management. In this context, use of circulating microRNAs (miRNAs) may serve as diagnostic tools as liquid biopsies, and as biomarkers to better understand the molecular mechanisms involved in the progression of cancer. We identified miR‐590‐5p as a potential prognostic marker in the progression of non‐small cell lung cancer (NSCLC). We were able to detect this miRNA in blood plasma samples of NSCLC patients through quantitative real‐time PCR. Our data showed an ~7.5‐fold downregulation of miR‐590‐5p in NSCLC patients compared to healthy controls, which correlated with several clinicopathological features. Further, overexpression of miR‐590‐5p led to decreased cell viability, proliferation, colony formation, migration, and invasion potential of lung cancer cells, whereas its knockdown showed the opposite effect. In addition, the levels of several proteins involved in the epithelial‐to‐mesenchymal transition negatively correlated with miR‐590‐5p levels in lung adenocarcinoma cells and tumors of NSCLC patients. Further, dual‐luciferase reporter assays identified STAT3 as a direct target of miR‐590‐5p, which negatively regulated STAT3 activation and its downstream signaling molecules (eg, Cyclin D1, c‐Myc, Vimentin, and β‐catenin) involved in tumorigenesis. Taken together, our study suggests that miR‐590‐5p functions as a tumor suppressor in NSCLC through regulating the STAT3 pathway, and may serve as a useful biomarker for the diagnosis/prognosis of NSCLC, and as a potential therapeutic target for the treatment of NSCLC.

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Section: Immunohistochemistrymentioning

confidence: 99%

Circulating microRNA‐590‐5p functions as a liquid biopsy marker in non‐small cell lung cancer

et al. 2020

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“…The stomach and esophagus have a dual microenvironment. It can easily lead to local epithelial mucosal repair errors when the in ammatory lesion leads to in ammation and immune cascade reaction, induces relevant immune factors and in ammatory factors to destroy the mucosa, and even develop into cancer [24]. Therefore, when the routine pathology of some patients cannot explain the occurrence and development of the disease, immunohistochemistry is often needed to assist the diagnosis and guide follow-up.…”

Section: Discussionmentioning

confidence: 99%

Necessity of pathological examination of reflux esophagitis

Chen

Linghu

Zhu

et al. 2022

Preprint

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Background: Routine biopsies are not recommended in the guidelines for RE patients, whereas upper gastrointestinal endoscopy combined with histopathological examination is necessary for RE patients with high-risk factors. Methods: The medical records of 613 patients with RE who had undergone upper gastrointestinal endoscopy and biopsy for histopathological examination were collected. Relevant and risk factors for pathological outcomes were analyzed. Result: The proportions of middle-aged and male participants were higher. The pathological outcome was related to age and endoscopic findings, but not sex. Age is a risk factor for intestinal metaplasia and is associated with esophagitis severity. Esophageal mucosa status, white moss, and hemostasis with adrenaline are relevant with LIN,only mucosal status is a risk factor. LA Grade is a significant risk factor for HIN. Mucosa status and white moss are both risk factors for erosive ulcer and pathological follow-up necessity. Conclusion: Biopsy and pathological follow-up of cases with risk factors, especially endoscopic mucosal abnormalities, help screen for precancerous lesions and reduce misdiagnosis.

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“…However, the (n + c)Maspin-expressing cells did not disappear with the basal epithelial layer. Instead, they are detected abundantly at the earliest in situ tumors [ 1 , 15 ] (Fig. 1Ba–c ), exhibiting a gene expression profile similar to that of epithelial precursor cells [ 16 ].…”

Section: The (N + C)maspin-expressing Cellular State In Stem Cells Di...mentioning

confidence: 99%

The vulnerable primed cancer stem cells in disguise: demystifying the role of Maspin

Shen

Bernardo

Dzinic

et al. 2022

Cancer Metastasis Rev

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Epithelial-specific Maspin is widely known as a tumor suppressor. However, while the level of maspin expression is inversely correlated with tumor grade and stage, emerging clinical evidence shows a correlation between seemingly better differentiated tumor cells that express Maspin in both the nucleus and the cytoplasm, (n + c)Maspin, with a poor prognosis of many types of cancer. Biological studies demonstrate that Maspin plays an essential role in stem cell differentiation. In light of the recently established characterization of primed stem cells (P-SCs) in development, we propose, for the first time, that cancer stem cells (CSCs) also need to undergo priming (P-CSCs) before their transition to various progeny phenotypes. We envisage major differences in the steady state kinetics between P-SCs and P-CSCs. We further propose that P-CSCs of carcinoma are both marked and regulated by (n + c)Maspin. The concept of P-CSCs helps explain the apparent dichotomous relationships of (n + c)Maspin expression with cancer diagnosis and prognosis, and is supported by the evidence from mechanistic studies. We believe that the potential utility of (n + c)Maspin as a molecular marker of P-CSCs may significantly accelerate the advancement in our understanding of the genesis of tumor phenotypic plasticity in response to changes of tumor microenvironments (TME) or drug treatments. The vulnerabilities of the cellular state of (n + c)Maspin-expressing P-CSCs are also discussed as the rationale for future development of P-CSC-targeted chemotherapeutic and immunotherapeutic strategies.

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Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma

Cited by 6 publications

References 47 publications

Circulating microRNA‐590‐5p functions as a liquid biopsy marker in non‐small cell lung cancer

Circulating microRNA‐590‐5p functions as a liquid biopsy marker in non‐small cell lung cancer

Necessity of pathological examination of reflux esophagitis

The vulnerable primed cancer stem cells in disguise: demystifying the role of Maspin

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