Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells

Abstract

Cutaneous squamous cell carcinoma (cSCC) is a common malignant tumor. Mammary serine protease inhibitor (Maspin), a member of serpin family, has been reported as a tumor suppressor in various carcinomas. In this study, we detected the expression level of Maspin in cSCC tissues by real-time PCR and western blotting, and found that Maspin was downregulated in the cSCC tissues compared with the adjacent normal tissues. Moreover, Maspin was stably overexpressed in A431 cells, and CCK-8 assay, colony formation assay, Transwell assay, Hoechst staining and western blotting were carried out to detect the growth, proliferation, invasion, cell cycle and apoptosis of A431 cells. The results revealed that overexpression of Maspin inhibited growth, proliferation, invasion and cell cycle G1/S/G2 transition and enhanced apoptosis of A431 cells. The pro-apoptotic protein cleaved caspase-3, poly(ADP-ribose) polymerase (PARP) and Bax increased, and the anti-apoptotic protein Bcl-2 decreased after Maspin overexpression. Therefore, we demonstrated that Maspin suppressed growth, proliferation and invasion by delaying cell cycle transition and promoting apoptosis in cSCC cells, which may provide new insights for the clinical diagnosis and therapy of cSCC.

Differential regulation of miR‐21‐5p delays wound healing of melanocyte‐deprived vitiligo skin by modulating the expression of tumor‐suppressors PDCD4 and Maspin

“…Although bioinformatics tools predicted multiple miR-21 targets, we focused on PDCD4 and Maspin, both tumor suppressors, as they are known to regulate cell proliferation and/or migration (Wang et al, 2015;H. Zhu et al, 2017).…”

“…Although bioinformatics tools predicted multiple miR‐21 targets, we focused on PDCD4 and Maspin, both tumor suppressors, as they are known to regulate cell proliferation and/or migration (Wang et al, 2015; H. Zhu et al, 2017). Furthermore, the expression of PDCD4 and Maspin was found to be consistent with the regulation pattern of miR‐21, both in the clinical samples as well as in the miR‐21 mimic/antagomiR transfected cells, thus confirming them to be targeted by miR‐21.…”

Squamous Cell Carcinoma: Biomarkers and Potential Therapeutic Targets

“…Studies performed on A431 cell line revealed that overexpression of Maspin inhibits growth, cellular proliferation and enhances A431 cells apoptosis by increasing PARP and Bax expression, while decreasing Bcl-2 expression. Therefore, Maspin analysis may provide new insights in the diagnosis and therapy of cSCC [93].…”

BTG2 and SerpinB5, a novel gene pair to evaluate the prognosis of lung adenocarcinoma

“…SerpinB5 was expressed in normal breast epithelial cells, skin, prostate, testis, lung, tongue, intestine and thymus, but the expression level was abnormally lower in a variety of malignant tumors compared with the expression level in normal tissue. Previous studies have shown that SerpinB5 can inhibit the occurrence and development of tumors, including promoting tumor cell apoptosis, inhibiting tumor angiogenesis, and inhibiting tumor metastasis (18)(19)(20).…”