Masquelet Technique for Treatment of Segmental Bone Loss in the Upper Extremity

Modified Masquelet technique using allogeneic umbilical cord-derived mesenchymal stem cells for infected non-union femoral shaft fracture with a 12 cm bone defect: A case report

“…We consider our procedure to be a modified Masquelet technique due to the fact that we use UC-MSCs implantation as a replacement of autogenous and synthetic bone graft. Current studies suggest that the induced membrane plays a role in osteogenesis and vascularization of bone graft [4], [6], [8], [10], [12], [14], [15], [16]. In our case, clinical union was achieved at 6 months post operatively and patient was able to mobilize with the help of axillary crutches.…”

“…In the first step, a thorough debridement is performed, the segmental bone defect is bridged using a polymethylmethacrylate (PMMA) cement spacer and the bone is stabilized using orthopaedic hardwares. In a grossly contaminated or infected wounds, more than one surgical debridement and temporary stabilization may be required [4], [11], [15], [16].…”

“…Recently, Masquelet proposed a simple method of treating bone defect by combining autologous bone grafting that is placed within induced granulation tissue membranes and is applicable to both aseptic and septic conditions. This technique requires no advanced skills in microvascular surgery [1], [3], [4].…”

Recombinant Human Bone Morphogenetic Protein 6 Delivered Within Autologous Blood Coagulum Restores Critical Size Segmental Defects of Ulna in Rabbits

“…Allogenic demineralized bone matrix (DBM) has been used widely as an option to induce new bone formation because it provides osteoinductive signals and osteoconductive substratum . Advances made in the isolation of bone morphogenetic proteins (BMP) from DBM and subsequently the identification of the corresponding genes allowed the production of recombinant human BMPs in promoting fracture healing at compromised settings . Recombinant BMP2 soaked in absorbable collagen sponge (InFuse) and recombinant BMP7 combined with bovine bone collagen (OP1‐Putty) have been approved as biologic bone graft substitutes to bridge the gap and restore delayed and nonunion fractures …”

“…(13)(14)(15) Advances made in the isolation of bone morphogenetic proteins (BMP) from DBM (16) and subsequently the identification of the corresponding genes (17,18) allowed the production of recombinant human BMPs in promoting fracture healing at compromised settings. (19)(20)(21) Recombinant BMP2 soaked in absorbable collagen sponge (InFuse) and recombinant BMP7 combined with bovine bone collagen (OP1-Putty) have been approved as biologic bone graft substitutes to bridge the gap and restore delayed and nonunion fractures. (22)(23)(24) BMP alone cannot form bone unless it is delivered with an appropriate scaffold and responding cells are available in a permissive environment.…”