Mass fabrication and delivery of 3D multilayer μTags into living cells

Chen, Lisa Y.; Parizi, Kokab B.; Kosuge, Hisanori; Milaninia, Kaveh M.; McConnell, Michael V.; Wong, H.-S. Philip; Poon, Ada S. Y.

doi:10.1038/srep02295

Cited by 14 publications

(20 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, here we have shown that with proper surface functionalization these cells can readily internalize WGM resonators with volumes 300-fold larger than this, thus revealing a surprising ability of cells to internalize large polymer spheres. We also demonstrated an increase in the dimensions of phagocytosed objects for macrophages, with earlier reports showing uptake of spherical objects with diameters of up to 12.5 μm2226 and eliptical disks of up to 18 μm × 7 μm × 4 μm2234. Importantly, our study shows that microresonator internalization is highly feasible for all cell types investigated here, including cells generally understood to be non-phagocytic, like HEK 293, N7, and SH-SY5Y.…”

Section: Discussionsupporting

confidence: 84%

Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators

Schubert

Volckaert

Karl

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Reliable methods to individually track large numbers of cells in real time are urgently needed to advance our understanding of important biological processes like cancer metastasis, neuronal network development and wound healing. It has recently been suggested to introduce microscopic whispering gallery mode lasers into the cytoplasm of cells and to use their characteristic, size-dependent emission spectrum as optical barcode but so far there is no evidence that this approach is generally applicable. Here, we describe a method that drastically improves intracellular delivery of resonators for several cell types, including mitotic and non-phagocytic cells. In addition, we characterize the influence of resonator size on the spectral characteristics of the emitted laser light and identify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneously. Finally, we observe that the microresonators remain internalized by cells during cell division, which enables tagging several generations of cells.

show abstract

Section: Discussionsupporting

confidence: 84%

Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators

Schubert

Volckaert

Karl

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…For our application, the device size and detection range have already been set. We limit the RFID diameter to be less than 25 µm, which is the typical size of mouse macrophages that have previously been demonstrated to internalize similar dummy RFID structures [19]. Since the RFIDs are flown through a thin microfluidic channel above the TRX detector, the detection range is a few micrometers.…”

Section: Theoretical Analysismentioning

confidence: 99%

“…To test the biocompatibility of the RFID, the confluent culture of mouse melanocytic melanoma cells was incubated with RFID tags for 42 hours and imaged at intervals of three minutes to capture cell uptake of the RFID chips in a procedure described in [19]. The cell concentration was about 2 × 10 3 cells/mL, and the RFID-to-cell ratio was about 1 to 1 .…”

Section: Biocompatibility Verificationmentioning

confidence: 99%

“…7] as the RFIDs from the silicon foundry. The house RFID fabrication and release processes are described in [18,19]. In brief, these RFIDs consist of two metal layers sandwiching a thin dielectric layer.…”

Section: Biocompatibility Verificationmentioning

confidence: 99%

“…The RFIDs are small enough to fit inside individual cells. They are fully encapsulated in SiO 2 for bio-compatibility [19].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Micrometer-Scale Magnetic-Resonance-Coupled Radio-Frequency Identification and Transceivers for Wireless Sensors in Cells

Aggarwal

Yang

et al. 2017

Phys. Rev. Applied

Self Cite

View full text Add to dashboard Cite

We report the design, analysis and characterization of a three-inductor radio-frequency identification (RFID) and transceiver (TRX) system for potential applications in individual cell tracking and monitoring. The RFID diameter is 22 µm and can be naturally internalized by living cells. Using magnetic resonant coupling, the system shows resonance shifts when the RFID is present and also when the RFID loading capacitance changes. It operates at 60 GHz with a high signal magnitude up to −50 dB and a sensitivity of 0.25. This miniaturized RFID with a high signal magnitude is a promising step toward continuous, real-time monitoring of activities at cellular levels.

show abstract

Digitalized Human Organoid for Wireless Phenotyping

et al. 2018

View full text Add to dashboard Cite

SummaryRadio frequency identification (RFID) is a cost-effective and durable method to trace and track individual objects in multiple contexts by wirelessly providing digital signals; RFID is thus widely used in many fields. Here, we implement this concept to biological tissues by producing a compact RFID chip-incorporated organoid (RiO). The 0.4 mm RFID chips are reproducibly integrated inside the self-assembling organoids from 10 different induced pluripotent stem cell (iPSC) lines from healthy and diseased donors. We use the digitalized RiO to conduct a phenotypic screen on a pool of RiO, followed by detection of each specific donor in situ. Our proof-of-principle experiments demonstrated that a severely steatotic phenotype could be identified by RFID chip reading and was specific to a genetic disorder of steatohepatitis. Given evolving advancements surrounding RFID technology, the digitalization principle outlined here will expand organoid medicine potential toward drug development, precision medicine, and transplant applications.

show abstract

Mass fabrication and delivery of 3D multilayer μTags into living cells

Cited by 14 publications

References 18 publications

Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators

Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators

Micrometer-Scale Magnetic-Resonance-Coupled Radio-Frequency Identification and Transceivers for Wireless Sensors in Cells

Digitalized Human Organoid for Wireless Phenotyping

Contact Info

Product

Resources

About