Mass fragmentographic assay for .DELTA.9-tetrahydrocannabinol in plasma

Rosenfeld, J. J.; Bowins, Bob.; Roberts, Jeff.; Perkins, John R.; Macpherson, Alexander S.

doi:10.1021/ac60350a047

Cited by 37 publications

(3 citation statements)

References 19 publications

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“…They are, however, comparatively less stable and dissolve significantly in aqueous solutions. Titanium vanadate (6) which has been synthesized in these laboratories has been found specific for strontium and is stable at high temperatures. As pure vanadates appear to be unstable, it is possible that substances such as vanadophosphates may be more stable.…”

Section: Resultsmentioning

confidence: 99%

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Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

Vinson¹,

Patel²,

Patel³

1977

Anal. Chem.

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A thin-layer chromatographic method Is described for the detection of A9-tetrahydrocannabinol in blood and serum. The procedure utilizes a reagent, 2-p-chlorosulfophenyl-3-phenylindone, which reacts with the phenolic group to form a derivative. Following extraction and cleanup, the derivative Is prepared and separated from reagent and naturally occurring compounds by thin-layer chromatography. The derivative is then detected by spraying the thin-layer plate with an alkoxide spray which produces a fluorescent spot visible under long wave ultraviolet light. The method can detect 0.2 ng/ml of tetrahydrocannabinol in 5 ml of serum and is suitable for routine screening.

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Section: Resultsmentioning

confidence: 99%

“…Bleeding from the column and natural interferences limit the selectivity and sensitivity of the method. Mass fragmentography used in combination with GC offers excellent sensitivity and selectivity (2,6). However, this method is too expensive for routine monitoring and requires considerable expertise.…”

mentioning

confidence: 99%

Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

Vinson¹,

Patel²,

Patel³

1977

Anal. Chem.

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“…Therefore, incorporation of stable isotopes (15N, D) in the molecule of interest has found wide acceptance in mass fragmentography, as the GC-MS combination is able to distinguish between chemically identical compounds on a purely physical basis (Samuelson et al, 1970;Gafney et al, 1971; Bertillson et al, 1972;Sjoquist and Anggard, 1972; Rosenfeld et al, 1974).…”

mentioning

confidence: 99%

Gas chromatographic determination of 2,4-dichlorophenoxyacetic acid (2,4-D) by mass fragmentography with a deuterated internal standard

Peteghem¹,

Am²

1976

J. Agric. Food Chem.

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Quantitative Massenspektrometrie in Biochemie und Medizin

Lehmann

Schulten

1978

Angew. Chem.

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Der vorliegende Aufsatz gibt einen uberblick iiber den Stand der quantitativen Analyse mit dem Massenspektrometer in Biochemie und Medizin. Die Grundlagen dafur, die gekoppelte Anwendung chromatographischer und massenspektrometrischer Methoden, die Entwicklung empfindlicher, genauer und iiberdies spezifischer massenspektrometrischer Nachweismethoden sowie das Prinzip der Verdiinnung mit stabilen Isotopen werden an Beispielen erlautert. Die wichtigsten Anwendungsgebiete sind die Verfolgung der Pharmakokinetik von Arzneimitteln und aktiven Metaboliten, die Erforschung von Stoffwechselwegen, die Unterstiitzung und Spezifizierung medizinischer Diagnosen und schlieI3lich die Qualitatskontrolle einfacherer quantitativer Verfahren der klinischen Chemie.

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Mass fragmentographic assay for .DELTA.9-tetrahydrocannabinol in plasma

Cited by 37 publications

References 19 publications

Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

Gas chromatographic determination of 2,4-dichlorophenoxyacetic acid (2,4-D) by mass fragmentography with a deuterated internal standard

Quantitative Massenspektrometrie in Biochemie und Medizin

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